SASDAL7

Metal-free hETHE1, 3 mg/ml

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2023-05-25T11:21:52.654103+02:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Metal-free hETHE1, 3 mg/ml

浓度— – 3.0 mg/ml缓冲液 / pH50 mM Tris 150 mM NaCl 2 mM TCEP / 8.0
Experimental temperature5.0 设备 / 束线DORIS III, DESY / EMBL X33
波长0.15 nm曝光30.0 s × 4

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
Persulfide dioxygenase ETHE1, mitochondrialproteinHomo sapiensO95571—–—dimer分子数 227.873 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
12310[3]0.0774543–6.23701 1/nm含误差列缺失 0212sasbdb/entries/l7/sasdal7/source/SASDAL7.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)56.0nm
estimated_volumeDAMMIN1656.0nm³
i0Guinier318.87
i0P(r)363.6
mwExperimental828.0kDa
mwPorod903.0kDa
porod_volumePorod1445.0nm³
rgGuinier11.21nm
rgP(r)14.12nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50 mM Tris 150 mM NaCl 2 mM TCEP缓冲液浓度50.0 mM
pH8.0添加剂150 mM NaCl 2 mM TCEP
缓冲液说明TCEP does not reduce metals in solution, but prevents aggregation and precipitation of the protein
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2009-11-23储存 / 测量温度— / 5.0
曝光时间30.0帧数4
波长0.15样品-探测器距离2.7
光源X-ray synchrotron探测器Pilatus 1M-W
机构 / 束线DORIS III, DESY / EMBL X33 · Hamburg, Germany
q range0.077 – 6.237样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:158 个字段值
字段路径原始值
codeSASDAL7
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleDistinctive features and structural significance of the Homo sapiens ethylmalonic encephalopathy protein iron binding site
project.publicationnull
project.statusreleased
project.submitted_date2015-02-17
project.released_date2017-02-08
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experiment.instrument.nameDORIS III, DESY
experiment.instrument.cityHamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL X33
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experiment.instrument.xray_energynull
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experiment.instrument.beam_profile_alnull
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experiment.sample.molecule[0].short_namehETHE1
experiment.sample.molecule[0].sequencenull
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeO95571
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.molecule[0].mw27.873
experiment.sample.molecule[0].total_mw55.746
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
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experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name50 mM Tris 150 mM NaCl 2 mM TCEP
experiment.sample.buffer.concentration_unitmM
experiment.sample.buffer.commentTCEP does not reduce metals in solution, but prevents aggregation and precipitation of the protein
experiment.sample.buffer.additive150 mM NaCl 2 mM TCEP
experiment.sample.buffer.concentration50.0
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameMetal-free hETHE1, 3 mg/ml
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameAl
experiment.contributor[0].contributor_surnameKikhney
experiment.contributor[0].orcidhttps://orcid.org/0000-0003-1321-3956
experiment.contributor[1].affiliation[0].short_namenull
experiment.contributor[1].affiliation[0].addressBolzano, Italy
experiment.contributor[1].affiliation[0].full_nameLibera Università di Bozen-Bolzano
experiment.contributor[1].affiliation[0].webpagehttps://www.unibz.it/
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experiment.contributor[1].contributor_surnameSalomone-Stagni
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experiment.concentration_methodnull
experiment.concentration_unitmg/ml
experiment.date2009-11-23
experiment.storage_temperaturenull
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experiment.exposure_time30.0
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experiment.flow_ratenull
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experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].fit_residual_plotSASDAL7_fit1_fitresiduals_img.png
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i0_calibration_standardnull
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guinier_rg_errornull
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porod_volume_errornull
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estimated_volume_errornull
guinier_point_first1
guinier_point_last13
pddf_point_firstnull
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i0_calibration_standard_datanull
intensities_log_log_plotSASDAL7_datloglog_img.png
symmetrynull
last_modified2023-05-25T11:21:52.654103+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDAL7
statussuccess
started_at2026-08-11T15:32:34.845426+00:00
finished_at2026-08-11T15:32:42.820361+00:00
source_last_modified2023-05-25T11:21:52.654103+02:00
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查看完整 summary.json 原文
{
  "code": "SASDAL7",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Distinctive features and structural significance of the Homo sapiens ethylmalonic encephalopathy protein iron binding site",
    "publication": null,
    "status": "released",
    "submitted_date": "2015-02-17",
    "released_date": "2017-02-08"
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}
查看完整 manifest.json 原文
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