SASDAP4

Chitinase 60 from Moritella marina

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2023-06-21T07:56:39.492710+02:00

The fit is obtained by the average of three EOM models

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Chitinase 60 from Moritella marina

浓度0.6 – 8.8 缓冲液 / pH20 mM Tris 200 mM NaCl / 8.0
Experimental temperature10.0 设备 / 束线PETRA III / EMBL P12
波长0.12 nm曝光1.0 s × —

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
Chitinase 60
查看序列
>TR|B1VBB0|B1VBB0_VIBMACHITINASE60OS=VIBRIOMARINUSGN=CHI60PE=1SV=1MKLKSILSAAIFTGLFSTAGIAGTITSQDDNVVVGYWHNWCDGRGYQGGNAPCVELKTVNPQYNVVNISFMKVYDIAEGRIPTFKLDPTIALSEAEFIAQIDTLNSQGRSVLIALGGADAHIELTRGDEDALAAEIIRLTDLYGFDGLDIDLEQAAITAKDNQFVIPAALKMVKEHYRKTGDNFMITMAPEFPYLTANGAYTPYLTELDGYYDFINPQFYNQGGDGLWIEGVGWIAQNNDALKEEFIYYIADSLINGTRNYHKIPHDKLVFGLPSNIDAAATGYIQDPQDLYKAFDRLKAQGQPLRGVMTWSVNWDMGTDAANNSYNQQFIKDYGNFIHNQLPPVTDMTPTLSGIVDTRVELDSHFDPLIGITAKDYQGNDITADVTVSGSVNTNQVGDYLLTYSVSSDDETTNQPRKITVYEILPAFTGITDTTVVIDSEFDPMQGVSASRPTQGDLTANITVTGEVDANVVGVYELTYQLFYGQDNQQNMTDKRIVTVVTDAVSDDDWQVGSTYVKDDKVTHNGATWTAQWWTKGEEPGTTGEWGVWR
proteinMoritella marinaB1VBB0—–—monomer分子数 160.79 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
11727[3]0.0702666–4.65245 1/nm含误差列缺失 0128sasbdb/entries/p4/sasdap4/source/SASDAP4.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)11.25nm
i0Guinier2850.0
mwExperimental50.0kDa
porod_volumePorod75.0nm³
rgGuinier3.2nm
rgP(r)3.37nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM Tris 200 mM NaCl缓冲液浓度
pH8.0添加剂NaCl 200 mM
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2013-03-18储存 / 测量温度10.0 / 10.0
曝光时间1.0帧数
波长0.12样品-探测器距离3.1
光源X-ray synchrotron探测器Pilatus 2M
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.07 – 4.652样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
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curve:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:182 个字段值
字段路径原始值
codeSASDAP4
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleCrystal structures of substrate-bound chitinase from the psychrophilic bacterium Moritella marina and its structure in solution.
project.publication.titleCrystal structures of substrate-bound chitinase from the psychrophilic bacterium Moritella marina and its structure in solution.
project.publication.author_listMalecki PH, Vorgias CE, Petoukhov MV, Svergun DI, Rypniewski W
project.publication.journalActa Crystallogr D Biol Crystallogr
project.publication.doi10.1107/S1399004713032264
project.publication.pmid24598737
project.publication.published_date2014 Mar
project.statusreleased
project.submitted_datenull
project.released_date2015-07-07
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameChitinase 60
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequence>tr|B1VBB0|B1VBB0_VIBMA Chitinase 60 OS=Vibrio marinus GN=chi60 PE=1 SV=1 MKLKSILSAAIFTGLFSTAGIAGTITSQDDNVVVGYWHNWCDGRGYQGGNAPCVELKTVN PQYNVVNISFMKVYDIAEGRIPTFKLDPTIALSEAEFIAQIDTLNSQGRSVLIALGGADA HIELTRGDEDALAAEIIRLTDLYGFDGLDIDLEQAAITAKDNQFVIPAALKMVKEHYRKT GDNFMITMAPEFPYLTANGAYTPYLTELDGYYDFINPQFYNQGGDGLWIEGVGWIAQNND ALKEEFIYYIADSLINGTRNYHKIPHDKLVFGLPSNIDAAATGYIQDPQDLYKAFDRLKA QGQPLRGVMTWSVNWDMGTDAANNSYNQQFIKDYGNFIHNQLPPVTDMTPTLSGIVDTRV ELDSHFDPLIGITAKDYQGNDITADVTVSGSVNTNQVGDYLLTYSVSSDDETTNQPRKIT VYEILPAFTGITDTTVVIDSEFDPMQGVSASRPTQGDLTANITVTGEVDANVVGVYELTY QLFYGQDNQQNMTDKRIVTVVTDAVSDDDWQVGSTYVKDDKVTHNGATWTAQWWTKGEEP GTTGEWGVWR
experiment.sample.molecule[0].organismMoritella marina
experiment.sample.molecule[0].uniprot_codeB1VBB0
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.molecule[0].mw60.79
experiment.sample.molecule[0].total_mw60.79
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_statenull
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM Tris 200 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additiveNaCl 200 mM
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameChitinase 60 from Moritella marina
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameMaxim
experiment.contributor[0].contributor_surnamePetoukhov
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2013-03-18
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time1.0
experiment.number_of_framesnull
experiment.wavelength0.12
experiment.sample_detector_distance3.1
experiment.concentration_min0.6
experiment.concentration_max8.8
experiment.sample_volumenull
experiment.flow_ratenull
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i0_calibration_standardnull
descriptionThe fit is obtained by the average of three EOM models
experiment_descriptionnull
tags[]
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last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDAP4
statussuccess
started_at2026-08-11T14:27:55.247135+00:00
finished_at2026-08-11T14:28:05.879439+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
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查看完整 summary.json 原文
{
  "code": "SASDAP4",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Crystal structures of substrate-bound chitinase from the psychrophilic bacterium Moritella marina and its structure in solution.",
    "publication": {
      "title": "Crystal structures of substrate-bound chitinase from the psychrophilic bacterium Moritella marina and its structure in solution.",
      "author_list": "Malecki PH, Vorgias CE, Petoukhov MV, Svergun DI, Rypniewski W",
      "journal": "Acta Crystallogr D Biol Crystallogr",
      "doi": "10.1107/S1399004713032264",
      "pmid": "24598737",
      "published_date": "2014 Mar"
    },
    "status": "released",
    "submitted_date": null,
    "released_date": "2015-07-07"
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查看完整 manifest.json 原文
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