SASDAP4

Chitinase 60 from Moritella marina

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2023-06-21T07:56:39.492710+02:00

The fit is obtained by the average of three EOM models

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Chitinase 60 from Moritella marina

Concentration0.6 – 8.8 Buffer / pH20 mM Tris 200 mM NaCl / 8.0
Experimental temperature10.0 Facility / beamlinePETRA III / EMBL P12
Wavelength0.12 nmExposure1.0 s × —

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Chitinase 60
View sequence
>TR|B1VBB0|B1VBB0_VIBMACHITINASE60OS=VIBRIOMARINUSGN=CHI60PE=1SV=1MKLKSILSAAIFTGLFSTAGIAGTITSQDDNVVVGYWHNWCDGRGYQGGNAPCVELKTVNPQYNVVNISFMKVYDIAEGRIPTFKLDPTIALSEAEFIAQIDTLNSQGRSVLIALGGADAHIELTRGDEDALAAEIIRLTDLYGFDGLDIDLEQAAITAKDNQFVIPAALKMVKEHYRKTGDNFMITMAPEFPYLTANGAYTPYLTELDGYYDFINPQFYNQGGDGLWIEGVGWIAQNNDALKEEFIYYIADSLINGTRNYHKIPHDKLVFGLPSNIDAAATGYIQDPQDLYKAFDRLKAQGQPLRGVMTWSVNWDMGTDAANNSYNQQFIKDYGNFIHNQLPPVTDMTPTLSGIVDTRVELDSHFDPLIGITAKDYQGNDITADVTVSGSVNTNQVGDYLLTYSVSSDDETTNQPRKITVYEILPAFTGITDTTVVIDSEFDPMQGVSASRPTQGDLTANITVTGEVDANVVGVYELTYQLFYGQDNQQNMTDKRIVTVVTDAVSDDDWQVGSTYVKDDKVTHNGATWTAQWWTKGEEPGTTGEWGVWR
proteinMoritella marinaB1VBB0—–—monomerMolecule count 160.79 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11727[3]0.0702666–4.65245 1/nmHas uncertainty columnMissing 0128sasbdb/entries/p4/sasdap4/source/SASDAP4.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)11.25—nm
i0Guinier2850.0——
mwExperimental50.0—kDa
porod_volumePorod75.0—nm³
rgGuinier3.2—nm
rgP(r)3.37—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM Tris 200 mM NaClBuffer concentration—
pH8.0AdditivesNaCl 200 mM
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2013-03-18Storage / measurement temperature10.0 / 10.0
Exposure time1.0Frames—
Wavelength0.12Sample-to-detector distance3.1
SourceX-ray synchrotronDetectorPilatus 2M
Facility / beamlinePETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.07 – 4.652Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:182 field values
Field pathRaw value
codeSASDAP4
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleCrystal structures of substrate-bound chitinase from the psychrophilic bacterium Moritella marina and its structure in solution.
project.publication.titleCrystal structures of substrate-bound chitinase from the psychrophilic bacterium Moritella marina and its structure in solution.
project.publication.author_listMalecki PH, Vorgias CE, Petoukhov MV, Svergun DI, Rypniewski W
project.publication.journalActa Crystallogr D Biol Crystallogr
project.publication.doi10.1107/S1399004713032264
project.publication.pmid24598737
project.publication.published_date2014 Mar
project.statusreleased
project.submitted_datenull
project.released_date2015-07-07
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experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameChitinase 60
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequence>tr|B1VBB0|B1VBB0_VIBMA Chitinase 60 OS=Vibrio marinus GN=chi60 PE=1 SV=1 MKLKSILSAAIFTGLFSTAGIAGTITSQDDNVVVGYWHNWCDGRGYQGGNAPCVELKTVN PQYNVVNISFMKVYDIAEGRIPTFKLDPTIALSEAEFIAQIDTLNSQGRSVLIALGGADA HIELTRGDEDALAAEIIRLTDLYGFDGLDIDLEQAAITAKDNQFVIPAALKMVKEHYRKT GDNFMITMAPEFPYLTANGAYTPYLTELDGYYDFINPQFYNQGGDGLWIEGVGWIAQNND ALKEEFIYYIADSLINGTRNYHKIPHDKLVFGLPSNIDAAATGYIQDPQDLYKAFDRLKA QGQPLRGVMTWSVNWDMGTDAANNSYNQQFIKDYGNFIHNQLPPVTDMTPTLSGIVDTRV ELDSHFDPLIGITAKDYQGNDITADVTVSGSVNTNQVGDYLLTYSVSSDDETTNQPRKIT VYEILPAFTGITDTTVVIDSEFDPMQGVSASRPTQGDLTANITVTGEVDANVVGVYELTY QLFYGQDNQQNMTDKRIVTVVTDAVSDDDWQVGSTYVKDDKVTHNGATWTAQWWTKGEEP GTTGEWGVWR
experiment.sample.molecule[0].organismMoritella marina
experiment.sample.molecule[0].uniprot_codeB1VBB0
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMKLKSILSAAIFTGLFSTAGIAGTITSQDDNVVVGYWHNWCDGRGYQGGNAPCVELKTVN PQYNVVNISFMKVYDIAEGRIPTFKLDPTIALSEAEFIAQIDTLNSQGRSVLIALGGADA HIELTRGDEDALAAEIIRLTDLYGFDGLDIDLEQAAITAKDNQFVIPAALKMVKEHYRKT GDNFMITMAPEFPYLTANGAYTPYLTELDGYYDFINPQFYNQGGDGLWIEGVGWIAQNND ALKEEFIYYIADSLINGTRNYHKIPHDKLVFGLPSNIDAAATGYIQDPQDLYKAFDRLKA QGQPLRGVMTWSVNWDMGTDAANNSYNQQFIKDYGNFIHNQLPPVTDMTPTLSGIVDTRV ELDSHFDPLIGITAKDYQGNDITADVTVSGSVNTNQVGDYLLTYSVSSDDETTNQPRKIT VYEILPAFTGITDTTVVIDSEFDPMQGVSASRPTQGDLTANITVTGEVDANVVGVYELTY QLFYGQDNQQNMTDKRIVTVVTDAVSDDDWQVGSTYVKDDKVTHNGATWTAQWWTKGEEP GTTGEWGVWR
experiment.sample.molecule[0].mw60.79
experiment.sample.molecule[0].total_mw60.79
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_statenull
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM Tris 200 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additiveNaCl 200 mM
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
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experiment.sample.purity_methodnull
experiment.sample.nameChitinase 60 from Moritella marina
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
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experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameMaxim
experiment.contributor[0].contributor_surnamePetoukhov
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2013-03-18
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time1.0
experiment.number_of_framesnull
experiment.wavelength0.12
experiment.sample_detector_distance3.1
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experiment_descriptionnull
tags[]
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last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
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statussuccess
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View the complete summary.json source
{
  "code": "SASDAP4",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Crystal structures of substrate-bound chitinase from the psychrophilic bacterium Moritella marina and its structure in solution.",
    "publication": {
      "title": "Crystal structures of substrate-bound chitinase from the psychrophilic bacterium Moritella marina and its structure in solution.",
      "author_list": "Malecki PH, Vorgias CE, Petoukhov MV, Svergun DI, Rypniewski W",
      "journal": "Acta Crystallogr D Biol Crystallogr",
      "doi": "10.1107/S1399004713032264",
      "pmid": "24598737",
      "published_date": "2014 Mar"
    },
    "status": "released",
    "submitted_date": null,
    "released_date": "2015-07-07"
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