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SASDAR7

DNA 22mer

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2023-06-21T07:56:39.492710+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · DNA 22mer

Concentration— – 1.5 mg/mlBuffer / pH20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT / 7.4
Experimental temperature— Facility / beamlinePETRA III / EMBL P12
Wavelength0.12 nmExposure0.05 s × 20

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
DNA 22mer
View sequence
5'-GCCTAGTGATTGACAGATTCCT-3'
DNA———–—monomerMolecule count 111.9 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11608[3]0.222245–4.45043 1/nmHas uncertainty columnMissing 042sasbdb/entries/r7/sasdar7/source/SASDAR7.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)7.5—nm
i0Guinier1260.0——
i0P(r)1277.0——
mwExperimental13.0—kDa
mwPorod19.0—kDa
porod_volumePorod19.0—nm³
rgGuinier2.2—nm
rgP(r)2.3—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTTBuffer concentration20.0 mM
pH7.4Additives150 mM NaCl, 5% glycerol, 1 mM DTT
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2013-12-06Storage / measurement temperature— / —
Exposure time0.05Frames20
Wavelength0.12Sample-to-detector distance3.1
SourceX-ray synchrotronDetectorPilatus 2M
Facility / beamlinePETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.222 – 4.45Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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full_entry_zip：source record and ZIP contents (4 items)
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pddf：source record
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sascif：source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json：156 field values
Field pathRaw value
codeSASDAR7
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleThe flexibility of a homeodomain transcription factor heterodimer and its allosteric regulation by DNA binding.
project.publication.titleThe flexibility of a homeodomain transcription factor heterodimer and its allosteric regulation by DNA binding.
project.publication.author_listMathiasen L, Valentini E, Boivin S, Cattaneo A, Blasi F, Svergun DI, Bruckmann C
project.publication.journalFEBS J
project.publication.doi10.1111/febs.13801
project.publication.pmid27390177
project.publication.published_date2016 Aug
project.statusreleased
project.submitted_datenull
project.released_date2016-07-25
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameDNA 22mer
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequence5'-GCCTAGTGATTGACAGATTCCT-3'
experiment.sample.molecule[0].organismnull
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeDNA
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw11.9
experiment.sample.molecule[0].total_mw11.9
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT
experiment.sample.buffer.concentration_unitmM
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive150 mM NaCl, 5% glycerol, 1 mM DTT
experiment.sample.buffer.concentration20.0
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameDNA 22mer
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameErica
experiment.contributor[0].contributor_surnameValentini
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitmg/ml
experiment.date2013-12-06
experiment.storage_temperaturenull
experiment.cell_temperaturenull
experiment.exposure_time0.05
experiment.number_of_frames20
experiment.wavelength0.12
experiment.sample_detector_distance3.1
experiment.concentration_minnull
experiment.concentration_max1.5
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.222
experiment.s_max4.45
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].softwarenull
fits[0].chi_square_value1.056784
fits[0].p_value0.529841
fits[0].fit_residual_plotSASDAR7_fit1_fitresiduals_img.png
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pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitnull
experimental_mw13.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw19.0
porod_mw_errornull
pddf_i01277.0
pddf_i0_errornull
guinier_i01260.0
guinier_i0_errornull
pddf_rg2.3
pddf_rg_errornull
guinier_rg2.2
guinier_rg_errornull
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pddf_dmax_errornull
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porod_volume_errornull
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estimated_volume_errornull
guinier_point_firstnull
guinier_point_lastnull
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDAR7_datloglog_img.png
symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json：36 field values
Field pathRaw value
codeSASDAR7
statussuccess
started_at2026-08-11T15:36:57.208180+00:00
finished_at2026-08-11T15:37:09.141095+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
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View the complete summary.json source
{
  "code": "SASDAR7",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "The flexibility of a homeodomain transcription factor heterodimer and its allosteric regulation by DNA binding.",
    "publication": {
      "title": "The flexibility of a homeodomain transcription factor heterodimer and its allosteric regulation by DNA binding.",
      "author_list": "Mathiasen L, Valentini E, Boivin S, Cattaneo A, Blasi F, Svergun DI, Bruckmann C",
      "journal": "FEBS J",
      "doi": "10.1111/febs.13801",
      "pmid": "27390177",
      "published_date": "2016 Aug"
    },
    "status": "released",
    "submitted_date": null,
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  },
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View the complete manifest.json source
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