SASDAR8

mLANA 124-316 mLBS1-2 8:1 complex

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2023-06-21T07:56:39.492710+02:00

Study of oligomeric states of mLANA and kLANA and their complexes with DNA

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · mLANA 124-316 mLBS1-2 8:1 complex

浓度— – 0.6 mg/ml缓冲液 / pH25 mM Na/K Phosphate / 7.5
Experimental temperature10.0 设备 / 束线PETRA III / EMBL P12
波长0.12 nm曝光0.05 s × 20

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
MHV-68 TR DNADNAunidentified herpesvirus—–—monomer分子数 130.0 kDa
Latency-associated nuclear antigen
查看序列
MPTSPPTTRNTTSGKTRSGCKRRCFNKPAAMPPKRRRAPKRPAPPPPPGCQGDEESSQGTQTPNPPSPPVPPSSPTLPSSPVPPSSPVHEPPSPSPPPAPPSPDVDVEGLDVGETDDPGPPPPKRYSRYQKPHNPSDPLPKKYQGMRRHLQVTAPRLFDPEGHPPTHFKSAVMFSSTHPYTLNKLHKCIQSKHVLSTPVSCLPLVPGTTQQCVTYYLLSFVEDKKQAKKLKRVVLAYCEKYHSSVEGTIVKAKPYFPLPEPPTEPPTDPEQPSTSTQASGTQHGPTASLDAGAEQGATGSPGSSPGQQGQGSQT
proteinMurid herpesvirus 4O41974124–316octamer分子数 833.579 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
11670[3]0.0591163–4.45043 1/nm含误差列缺失 0525sasbdb/entries/r8/sasdar8/source/SASDAR8.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)20.0nm
i0Guinier11755.0
mwExperimental290.0kDa
mwGuinier I(0)290.0kDa
mwPorod290.0kDa
porod_volumePorod475.0nm³
rgGuinier5.8nm
rgP(r)6.0nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称25 mM Na/K Phosphate缓冲液浓度
pH7.5添加剂
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2013-04-27储存 / 测量温度— / 10.0
曝光时间0.05帧数20
波长0.12样品-探测器距离3.1
光源X-ray synchrotron探测器Pilatus 2M
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.059 – 4.45样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
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pddfsasbdb/entries/r8/sasdar8/source/SASDAR8.outdownloaded41745330df52f9fbc9dd80d64e2f371c58222917da568e1b7334bf980948553146eb8Download查看原文件源站
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:173 个字段值
字段路径原始值
codeSASDAR8
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleKSHV but not MHV-68 LANA induces a strong bend upon binding to terminal repeat viral DNA.
project.publication.titleKSHV but not MHV-68 LANA induces a strong bend upon binding to terminal repeat viral DNA.
project.publication.author_listPonnusamy R, Petoukhov MV, Correia B, Custodio TF, Juillard F, Tan M, Pires de Miranda M, Carrondo MA, Simas JP, Kaye KM, Svergun DI, McVey CE
project.publication.journalNucleic Acids Res
project.publication.doi10.1093/nar/gkv987
project.publication.pmid26424851
project.publication.published_date2015 Nov 16
project.statusreleased
project.submitted_date2015-07-06
project.released_date2016-03-17
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experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameMHV-68 TR DNA
experiment.sample.molecule[0].short_namemLBS1-2
experiment.sample.molecule[0].sequencenull
experiment.sample.molecule[0].organismunidentified herpesvirus
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeDNA
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw30.0
experiment.sample.molecule[0].total_mw30.0
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_nameLatency-associated nuclear antigen
experiment.sample.molecule[1].short_namemLANA
experiment.sample.molecule[1].sequenceMPTSPPTTRNTTSGKTRSGCKRRCFNKPAAMPPKRRRAPKRPAPPPPPGCQGDEESSQGTQTPNPPSPPVPPSSPTLPSSPVPPSSPVHEPPSPSPPPAPPSPDVDVEGLDVGETDDPGPPPPKRYSRYQKPHNPSDPLPKKYQGMRRHLQVTAPRLFDPEGHPPTHFKSAVMFSSTHPYTLNKLHKCIQSKHVLSTPVSCLPLVPGTTQQCVTYYLLSFVEDKKQAKKLKRVVLAYCEKYHSSVEGTIVKAKPYFPLPEPPTEPPTDPEQPSTSTQASGTQHGPTASLDAGAEQGATGSPGSSPGQQGQGSQT
experiment.sample.molecule[1].organismMurid herpesvirus 4
experiment.sample.molecule[1].uniprot_codeO41974
experiment.sample.molecule[1].uniprot_range_first124
experiment.sample.molecule[1].uniprot_range_last316
experiment.sample.molecule[1].oligomerizationoctamer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequenceMPTSPPTTRNTTSGKTRSGCKRRCFNKPAAMPPKRRRAPKRPAPPPPPGCQGDEESSQGT QTPNPPSPPVPPSSPTLPSSPVPPSSPVHEPPSPSPPPAPPSPDVDVEGLDVGETDDPGP PPPKRYSRYQKPHNPSDPLPKKYQGMRRHLQVTAPRLFDPEGHPPTHFKSAVMFSSTHPY TLNKLHKCIQSKHVLSTPVSCLPLVPGTTQQCVTYYLLSFVEDKKQAKKLKRVVLAYCEK YHSSVEGTIVKAKPYFPLPEPPTEPPTDPEQPSTSTQASGTQHGPTASLDAGAEQGATGS PGSSPGQQGQGSQT
experiment.sample.molecule[1].mw33.579
experiment.sample.molecule[1].total_mw268.635
experiment.sample.molecule[1].number_molecules8
experiment.sample.molecule[1].complex_statenull
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
experiment.sample.buffer.name25 mM Na/K Phosphate
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.namemLANA 124-316 mLBS1-2 8:1 complex
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameMaxim
experiment.contributor[0].contributor_surnamePetoukhov
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitmg/ml
experiment.date2013-04-27
experiment.storage_temperaturenull
experiment.cell_temperature10.0
experiment.exposure_time0.05
experiment.number_of_frames20
experiment.wavelength0.12
experiment.sample_detector_distance3.1
experiment.concentration_minnull
experiment.concentration_max0.6
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.059
experiment.s_max4.45
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].chi_square_value1.012
fits[0].p_value0.238333
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fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionStudy of oligomeric states of mLANA and kLANA and their complexes with DNA
experiment_descriptionnull
tags[]
intensity_unitnull
experimental_mw290.0
experimental_mw_errornull
guinier_i0_mw290.0
guinier_i0_mw_errornull
porod_mw290.0
porod_mw_errornull
pddf_i0null
pddf_i0_errornull
guinier_i011755.0
guinier_i0_errornull
pddf_rg6.0
pddf_rg_errornull
guinier_rg5.8
guinier_rg_errornull
pddf_dmax20.0
pddf_dmax_errornull
porod_volume475.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_firstnull
guinier_point_lastnull
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDAR8_datloglog_img.png
symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDAR8
statussuccess
started_at2026-08-11T15:58:54.278916+00:00
finished_at2026-08-11T15:59:03.798602+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
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查看完整 summary.json 原文
{
  "code": "SASDAR8",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "KSHV but not MHV-68 LANA induces a strong bend upon binding to terminal repeat viral DNA.",
    "publication": {
      "title": "KSHV but not MHV-68 LANA induces a strong bend upon binding to terminal repeat viral DNA.",
      "author_list": "Ponnusamy R, Petoukhov MV, Correia B, Custodio TF, Juillard F, Tan M, Pires de Miranda M, Carrondo MA, Simas JP, Kaye KM, Svergun DI, McVey CE",
      "journal": "Nucleic Acids Res",
      "doi": "10.1093/nar/gkv987",
      "pmid": "26424851",
      "published_date": "2015 Nov 16"
    },
    "status": "released",
    "submitted_date": "2015-07-06",
    "released_date": "2016-03-17"
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    "sample": {
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          "short_name": "mLBS1-2 ",
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          "organism": "unidentified herpesvirus",
          "uniprot_code": null,
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          "oligomerization": "monomer",
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            "short_name": "EMBL-Hamburg",
            "address": "Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany",
            "full_name": "European Molecular Biology Laboratory (EMBL) - Hamburg outstation",
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  "last_modified": "2023-06-21T07:56:39.492710+02:00",
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}
查看完整 manifest.json 原文
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