SASDAU5

ad11 Fab + NGF

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2023-05-25T11:22:16.556436+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · ad11 Fab + NGF

Concentration— – — Buffer / pH30 mM Na-phosphate 75 mM NaCl / 7.0
Experimental temperature15.0 Facility / beamlineDORIS III, DESY / EMBL X33
Wavelength— nmExposure120.0 s × 2

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
aD11 Fab
View sequence
DIQMTQSPASLSASLGETVTIECRASEDIYNALAWYQQKPGKSPQLLIYNTDTLHTGVPSRFSGSGSGTQYSLKINSLQSEDVASYFCQHYFGYPRTFGGGTKLELKRADAAPTVSIFPPSSEQLASGGASVVCLLNNFYPKDISVKWKIDGSERQNGVLDSVTDQDSKDSTYSMSSTLTLTKAEYESHNSYTCEVTHKTSTSPVVKSFNRGEQVQLKESGPGLVQPSQTLSLTCTVSGFSLTNNNVNWVRQATGRGLEWMGGVWAGGATDYNSALKSRLTITRDTSKSQVFLKMHSLQSSDTATYYCARDGGYSSSTLYAMDAWGQGTTVTVSSASTTAPSVYPLAPGSMVTLGCLVKGYFPEPVTVTWNSGSLASGVHTFPAVLQSGLYTLSSSVTVPASPWASEAVTCNVAHPASSTKVDKKIVPR
proteinMus musculus——–—dimerMolecule count 225.0 kDa
NGF
View sequence
GEFSVCDSVSVWVGDKTTATDIKGKEVTVLAEVNINNSVFRQYFFETKCRASNPVESGCRGIDSKHWNSYCTTTHTFVKALTTDEKQAAWRFIRIDTACVCVLSRKAGEFSVCDSVSVWVGDKTTATDIKGKEVTVLAEVNINNSVFRQYFFETKCRASNPVESGCRGIDSKHWNSYCTTTHTFVKALTTDEKQAAWRFIRIDTACVCVLSRKA
proteinMus musculus——–—dimerMolecule count 225.0 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
12092[3]0.151706–4.85583 1/nmHas uncertainty columnMissing 0483sasbdb/entries/u5/sasdau5/source/SASDAU5.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)16.0—nm
i0Guinier2365600.0——
i0P(r)2365400.0——
mwExperimental130.0—kDa
porod_volumePorod255.0—nm³
rgGuinier4.3—nm
rgP(r)4.3—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name30 mM Na-phosphate 75 mM NaClBuffer concentration—
pH7.0Additives75 mM NaCl 75.000 mM
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2005-12-20Storage / measurement temperature15.0 / 15.0
Exposure time120.0Frames2
Wavelength—Sample-to-detector distance—
SourceX-ray synchrotronDetectorMAR 345 Image Plate
Facility / beamlineDORIS III, DESY / EMBL X33 · Hamburg, Germany
q range0.152 – 4.856Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:173 field values
Field pathRaw value
codeSASDAU5
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleDissecting NGF interactions with TrkA and p75 receptors by structural and functional studies of an anti-NGF neutralizing antibody.
project.publication.titleDissecting NGF interactions with TrkA and p75 receptors by structural and functional studies of an anti-NGF neutralizing antibody.
project.publication.author_listCovaceuszach S, Cassetta A, Konarev PV, Gonfloni S, Rudolph R, Svergun DI, Lamba D, Cattaneo A
project.publication.journalJ Mol Biol
project.publication.doi10.1016/j.jmb.2008.06.008
project.publication.pmid18635195
project.publication.published_date2008 Sep 12
project.statusreleased
project.submitted_datenull
project.released_date2015-07-07
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDAU5.out
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experiment.instrument.detector.typenull
experiment.instrument.detector.nameMAR 345 Image Plate
experiment.instrument.detector.resolutionnull
experiment.instrument.nameDORIS III, DESY
experiment.instrument.cityHamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL X33
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameaD11 Fab
experiment.sample.molecule[0].short_nameFab
experiment.sample.molecule[0].sequenceDIQMTQSPASLSASLGETVTIECRASEDIYNALAWYQQKPGKSPQLLIYNTDTLHTGVPSRFSGSGSGTQYSLKINSLQS EDVASYFCQHYFGYPRTFGGGTKLELKRADAAPTVSIFPPSSEQLASGGASVVCLLNNFYPKDISVKWKIDGSERQNGVL DSVTDQDSKDSTYSMSSTLTLTKAEYESHNSYTCEVTHKTSTSPVVKSFNRGEQVQLKESGPGLVQPSQTLSLTCTVSGF SLTNNNVNWVRQATGRGLEWMGGVWAGGATDYNSALKSRLTITRDTSKSQVFLKMHSLQSSDTATYYCARDGGYSSSTLY AMDAWGQGTTVTVSSASTTAPSVYPLAPGSMVTLGCLVKGYFPEPVTVTWNSGSLASGVHTFPAVLQSGLYTLSSSVTVP ASPWASEAVTCNVAHPASSTKVDKKIVPR
experiment.sample.molecule[0].organismMus musculus
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw25.0
experiment.sample.molecule[0].total_mw50.0
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_statenull
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_nameNGF
experiment.sample.molecule[1].short_nameNGF
experiment.sample.molecule[1].sequenceGEFSVCDSVSVWVGDKTTATDIKGKEVTVLAEVNINNSVFRQYFFETKCRASNPVESGCRGIDSKHWNSYCTTTHTFVKA LTTDEKQAAWRFIRIDTACVCVLSRKAGEFSVCDSVSVWVGDKTTATDIKGKEVTVLAEVNINNSVFRQYFFETKCRASN PVESGCRGIDSKHWNSYCTTTHTFVKALTTDEKQAAWRFIRIDTACVCVLSRKA
experiment.sample.molecule[1].organismMus musculus
experiment.sample.molecule[1].uniprot_codenull
experiment.sample.molecule[1].uniprot_range_firstnull
experiment.sample.molecule[1].uniprot_range_lastnull
experiment.sample.molecule[1].oligomerizationdimer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequencenull
experiment.sample.molecule[1].mw25.0
experiment.sample.molecule[1].total_mw50.0
experiment.sample.molecule[1].number_molecules2
experiment.sample.molecule[1].complex_statenull
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
experiment.sample.buffer.name30 mM Na-phosphate 75 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive75 mM NaCl 75.000 mM
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.namead11 Fab + NGF
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_namePetr
experiment.contributor[0].contributor_surnameKonarev
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2005-12-20
experiment.storage_temperature15.0
experiment.cell_temperature15.0
experiment.exposure_time120.0
experiment.number_of_frames2
experiment.wavelengthnull
experiment.sample_detector_distancenull
experiment.concentration_minnull
experiment.concentration_maxnull
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.152
experiment.s_max4.856
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].softwarenull
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fits[0].p_value0.000117
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fits[0].descriptionnull
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pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[0]X33
intensity_unitnull
experimental_mw130.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i02365400.0
pddf_i0_errornull
guinier_i02365600.0
guinier_i0_errornull
pddf_rg4.3
pddf_rg_errornull
guinier_rg4.3
guinier_rg_errornull
pddf_dmax16.0
pddf_dmax_errornull
porod_volume255.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_firstnull
guinier_point_lastnull
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDAU5_datloglog_img.png
symmetrynull
last_modified2023-05-25T11:22:16.556436+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDAU5
statussuccess
started_at2026-08-11T14:56:16.202041+00:00
finished_at2026-08-11T14:56:25.389082+00:00
source_last_modified2023-05-25T11:22:16.556436+02:00
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View the complete summary.json source
{
  "code": "SASDAU5",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Dissecting NGF interactions with TrkA and p75 receptors by structural and functional studies of an anti-NGF neutralizing antibody.",
    "publication": {
      "title": "Dissecting NGF interactions with TrkA and p75 receptors by structural and functional studies of an anti-NGF neutralizing antibody.",
      "author_list": "Covaceuszach S, Cassetta A, Konarev PV, Gonfloni S, Rudolph R, Svergun DI, Lamba D, Cattaneo A",
      "journal": "J Mol Biol",
      "doi": "10.1016/j.jmb.2008.06.008",
      "pmid": "18635195",
      "published_date": "2008 Sep 12"
    },
    "status": "released",
    "submitted_date": null,
    "released_date": "2015-07-07"
  },
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      "country": "Germany",
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      "type_of_source": "X-ray synchrotron",
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    },
    "sample": {
      "molecule": [
        {
          "long_name": "aD11 Fab",
          "short_name": "Fab",
          "sequence": "DIQMTQSPASLSASLGETVTIECRASEDIYNALAWYQQKPGKSPQLLIYNTDTLHTGVPSRFSGSGSGTQYSLKINSLQS\r\nEDVASYFCQHYFGYPRTFGGGTKLELKRADAAPTVSIFPPSSEQLASGGASVVCLLNNFYPKDISVKWKIDGSERQNGVL\r\nDSVTDQDSKDSTYSMSSTLTLTKAEYESHNSYTCEVTHKTSTSPVVKSFNRGEQVQLKESGPGLVQPSQTLSLTCTVSGF\r\nSLTNNNVNWVRQATGRGLEWMGGVWAGGATDYNSALKSRLTITRDTSKSQVFLKMHSLQSSDTATYYCARDGGYSSSTLY\r\nAMDAWGQGTTVTVSSASTTAPSVYPLAPGSMVTLGCLVKGYFPEPVTVTWNSGSLASGVHTFPAVLQSGLYTLSSSVTVP\r\nASPWASEAVTCNVAHPASSTKVDKKIVPR\r\n",
          "organism": "Mus musculus",
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": null,
          "mw": 25.0,
          "total_mw": 50.0,
          "number_molecules": 2,
          "complex_state": null,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        },
        {
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