SASDAV4

uPAR wild-type AE105 complex

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Merged 最后更新:2023-05-25T11:21:52.654103+02:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · uPAR wild-type AE105 complex

浓度1.5 – 4.4 mg/ml缓冲液 / pH25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4 / 7.2
Experimental temperature10.0 设备 / 束线DORIS III, DESY / EMBL X33
波长0.15 nm曝光30.0 s × 4

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
Urokinase plasminogen activator surface receptor
查看序列
>SP|Q03405|UPAR_HUMANUROKINASEPLASMINOGENACTIVATORSURFACERECEPTOROS=HOMOSAPIENSGN=PLAURPE=1SV=1MGHPPLLPLLLLLHTCVPASWGLRCMQCKTNGDCRVEECALGQDLCRTTIVRLWEEGEELELVEKSCTHSEKTNRTLSYRTGLKITSLTEVVCGLDLCNQGNSGRAVTYSRSRYLECISCGSSDMSCERGRHQSLQCRSPEEQCLDVVTHWIQEGEEGRPKDDRHLRGCGYLPGCPGSNGFHNNDTFHFLKCCNTTKCNEGPILELENLPQNGRQCYSCKGNSTHGCSSEETFLIDCRGPMNQCLVATGTHEPKNQSYMVRGCATASMCQHAHLGDAFSMNHIDVSCCTKSGCNHPDLDVQYRSGAAPQPGPAHLSLTITLLMTARLWGGTLLWT
proteinHomo sapiensQ03405—–—monomer分子数 136.98 kDa
synthetic peptide AE105
查看序列
DXFSRYLWS
protein—–—monomer分子数 11.18 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
12339[3]0.06746–6.345 1/nm含误差列缺失 0853sasbdb/entries/v4/sasdav4/source/SASDAV4.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)8.5nm
i0Guinier39.2
mwExperimental37.0kDa
mwGuinier I(0)37.0kDa
porod_volumePorod61.0nm³
rgGuinier2.5nm
rgP(r)2.5nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4缓冲液浓度25.0 mM
pH7.2添加剂Glycerol 5.000 %, NaSO4 50.000 mM
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2010-06-10储存 / 测量温度10.0 / 10.0
曝光时间30.0帧数4
波长0.15样品-探测器距离2.7
光源X-ray synchrotron探测器Pilatus 1M-W
机构 / 束线DORIS III, DESY / EMBL X33 · Hamburg, Germany
q range0.067 – 6.345样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
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full_entry_zipsasbdb/entries/v4/sasdav4/source/SASDAV4.zipdownloaded33366fb5db2a06e97a325e0577049010c1a7838eb758753621ad5029b08b15b3271c0Download查看原文件源站
pddfsasbdb/entries/v4/sasdav4/source/SASDAV4.outdownloaded330416466ca04283ebc4d639b5daa512a43e09d90cfd152371be0132affcefdef86e5Download查看原文件源站
sascifsasbdb/entries/v4/sasdav4/source/SASDAV4.sascifdownloaded150665e0478ad3cd94eebadaf9bdb01e1efd18758832b777f68d05247a9be0b76b46d0Download查看原文件源站
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:151 个字段值
字段路径原始值
codeSASDAV4
statusPublished
type_of_curveMerged
angular_unit1/nm
project.titleA flexible multidomain structure drives the function of the urokinase-type plasminogen activator receptor (uPAR).
project.publication.titleA flexible multidomain structure drives the function of the urokinase-type plasminogen activator receptor (uPAR).
project.publication.author_listMertens HD, Kjaergaard M, Mysling S, Gårdsvoll H, Jørgensen TJ, Svergun DI, Ploug M
project.publication.journalJ Biol Chem
project.publication.doi10.1074/jbc.M112.398404
project.publication.pmid22896701
project.publication.published_date2012 Oct 5
project.statusreleased
project.submitted_datenull
project.released_date2015-07-07
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDAV4.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDAV4.dat
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experiment.instrument.detector.typeCustom-built
experiment.instrument.detector.namePilatus 1M-W
experiment.instrument.detector.resolution0.172
experiment.instrument.nameDORIS III, DESY
experiment.instrument.cityHamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL X33
experiment.instrument.beam_geometry0.60
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameUrokinase plasminogen activator surface receptor
experiment.sample.molecule[0].short_nameuPAR
experiment.sample.molecule[0].sequence>sp|Q03405|UPAR_HUMAN Urokinase plasminogen activator surface receptor OS=Homo sapiens GN=PLAUR PE=1 SV=1 MGHPPLLPLLLLLHTCVPASWGLRCMQCKTNGDCRVEECALGQDLCRTTIVRLWEEGEEL ELVEKSCTHSEKTNRTLSYRTGLKITSLTEVVCGLDLCNQGNSGRAVTYSRSRYLECISC GSSDMSCERGRHQSLQCRSPEEQCLDVVTHWIQEGEEGRPKDDRHLRGCGYLPGCPGSNG FHNNDTFHFLKCCNTTKCNEGPILELENLPQNGRQCYSCKGNSTHGCSSEETFLIDCRGP MNQCLVATGTHEPKNQSYMVRGCATASMCQHAHLGDAFSMNHIDVSCCTKSGCNHPDLDV QYRSGAAPQPGPAHLSLTITLLMTARLWGGTLLWT
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeQ03405
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMGHPPLLPLLLLLHTCVPASWGLRCMQCKTNGDCRVEECALGQDLCRTTIVRLWEEGEEL ELVEKSCTHSEKTNRTLSYRTGLKITSLTEVVCGLDLCNQGNSGRAVTYSRSRYLECISC GSSDMSCERGRHQSLQCRSPEEQCLDVVTHWIQEGEEGRPKDDRHLRGCGYLPGCPGSNG FHNNDTFHFLKCCNTTKCNEGPILELENLPQNGRQCYSCKGNSTHGCSSEETFLIDCRGP MNQCLVATGTHEPKNQSYMVRGCATASMCQHAHLGDAFSMNHIDVSCCTKSGCNHPDLDV QYRSGAAPQPGPAHLSLTITLLMTARLWGGTLLWT
experiment.sample.molecule[0].mw36.98
experiment.sample.molecule[0].total_mw36.98
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_statenull
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_namesynthetic peptide AE105
experiment.sample.molecule[1].short_nameAE105
experiment.sample.molecule[1].sequenceDXFsrYLWS
experiment.sample.molecule[1].organismnull
experiment.sample.molecule[1].uniprot_codenull
experiment.sample.molecule[1].uniprot_range_firstnull
experiment.sample.molecule[1].uniprot_range_lastnull
experiment.sample.molecule[1].oligomerizationmonomer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequencenull
experiment.sample.molecule[1].mw1.18
experiment.sample.molecule[1].total_mw1.18
experiment.sample.molecule[1].number_molecules1
experiment.sample.molecule[1].complex_statenull
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
experiment.sample.buffer.name25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4
experiment.sample.buffer.concentration_unitmM
experiment.sample.buffer.commentnull
experiment.sample.buffer.additiveGlycerol 5.000 %, NaSO4 50.000 mM
experiment.sample.buffer.concentration25.0
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.2
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameuPAR wild-type AE105 complex
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameHaydyn
experiment.contributor[0].contributor_surnameMertens
experiment.contributor[0].orcidnull
experiment.concentration_methodA280
experiment.concentration_unitmg/ml
experiment.date2010-06-10
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time30.0
experiment.number_of_frames4
experiment.wavelength0.15
experiment.sample_detector_distance2.7
experiment.concentration_min1.5
experiment.concentration_max4.4
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.067
experiment.s_max6.345
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardBSA
descriptionnull
experiment_descriptionnull
tags[0]X33
intensity_unitnull
experimental_mw37.0
experimental_mw_errornull
guinier_i0_mw37.0
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i0null
pddf_i0_errornull
guinier_i039.2
guinier_i0_errornull
pddf_rg2.5
pddf_rg_errornull
guinier_rg2.5
guinier_rg_errornull
pddf_dmax8.5
pddf_dmax_errornull
porod_volume61.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first70
guinier_point_last167
pddf_point_first70
pddf_point_last600
i0_calibration_standard_datanull
intensities_log_log_plotSASDAV4_datloglog_img.png
symmetrynull
last_modified2023-05-25T11:21:52.654103+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDAV4
statussuccess
started_at2026-08-11T14:32:02.724843+00:00
finished_at2026-08-11T14:32:11.254921+00:00
source_last_modified2023-05-25T11:21:52.654103+02:00
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查看完整 summary.json 原文
{
  "code": "SASDAV4",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/nm",
  "project": {
    "title": "A flexible multidomain structure drives the function of the urokinase-type plasminogen activator receptor (uPAR).",
    "publication": {
      "title": "A flexible multidomain structure drives the function of the urokinase-type plasminogen activator receptor (uPAR).",
      "author_list": "Mertens HD, Kjaergaard M, Mysling S, Gårdsvoll H, Jørgensen TJ, Svergun DI, Ploug M",
      "journal": "J Biol Chem",
      "doi": "10.1074/jbc.M112.398404",
      "pmid": "22896701",
      "published_date": "2012 Oct 5"
    },
    "status": "released",
    "submitted_date": null,
    "released_date": "2015-07-07"
  },
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  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDAV4.dat",
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        "name": "Pilatus 1M-W",
        "resolution": 0.172
      },
      "name": "DORIS III, DESY",
      "city": "Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL X33",
      "beam_geometry": "0.60",
      "type_of_source": "X-ray synchrotron",
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      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
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    "sample": {
      "molecule": [
        {
          "long_name": "Urokinase plasminogen activator surface receptor",
          "short_name": "uPAR",
          "sequence": ">sp|Q03405|UPAR_HUMAN Urokinase plasminogen activator surface receptor OS=Homo sapiens GN=PLAUR PE=1 SV=1\nMGHPPLLPLLLLLHTCVPASWGLRCMQCKTNGDCRVEECALGQDLCRTTIVRLWEEGEEL\nELVEKSCTHSEKTNRTLSYRTGLKITSLTEVVCGLDLCNQGNSGRAVTYSRSRYLECISC\nGSSDMSCERGRHQSLQCRSPEEQCLDVVTHWIQEGEEGRPKDDRHLRGCGYLPGCPGSNG\nFHNNDTFHFLKCCNTTKCNEGPILELENLPQNGRQCYSCKGNSTHGCSSEETFLIDCRGP\nMNQCLVATGTHEPKNQSYMVRGCATASMCQHAHLGDAFSMNHIDVSCCTKSGCNHPDLDV\nQYRSGAAPQPGPAHLSLTITLLMTARLWGGTLLWT\n\n",
          "organism": "Homo sapiens",
          "uniprot_code": "Q03405",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MGHPPLLPLLLLLHTCVPASWGLRCMQCKTNGDCRVEECALGQDLCRTTIVRLWEEGEEL\nELVEKSCTHSEKTNRTLSYRTGLKITSLTEVVCGLDLCNQGNSGRAVTYSRSRYLECISC\nGSSDMSCERGRHQSLQCRSPEEQCLDVVTHWIQEGEEGRPKDDRHLRGCGYLPGCPGSNG\nFHNNDTFHFLKCCNTTKCNEGPILELENLPQNGRQCYSCKGNSTHGCSSEETFLIDCRGP\nMNQCLVATGTHEPKNQSYMVRGCATASMCQHAHLGDAFSMNHIDVSCCTKSGCNHPDLDV\nQYRSGAAPQPGPAHLSLTITLLMTARLWGGTLLWT",
          "mw": 36.98,
          "total_mw": 36.98,
          "number_molecules": 1,
          "complex_state": null,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        },
        {
          "long_name": "synthetic peptide AE105",
          "short_name": "AE105",
          "sequence": "DXFsrYLWS",
          "organism": null,
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
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查看完整 manifest.json 原文
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