SASDAW8

2:1 YopM:DDX3 complex

Data type: SASBDB experimental data Status: Published Curve type: Extrapolated to infinite dilution Last updated: 2023-06-21T07:56:39.492710+02:00

A complex composed of two YopM_34-481 molecules and one DDX3_51-418

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · 2:1 YopM:DDX3 complex

Concentration0.66 – 10.6 mg/mlBuffer / pH50 mM HEPES 150 mM NaCl / 7.5
Experimental temperature23.0 Facility / beamlinePETRA III / EMBL P12
Wavelength0.12 nmExposure0.05 s × 20

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Yersinia outer protein M (34-481)
View sequence
KSKTEYYNAWSEWERNAPPGNGEQREMAVSRLRDCLDRQAHELELNNLGLSSLPELPPHLERLVASCNSLTELPELPQSLKSLEVYENNLKALPDLPPLLVDLRVFNNQLEELPELQNLPFLTEIYANNNSLKTLPDLPPSLVDLNVRENYLTALPELPQSLIFLDISDNILSGLSELPPNLSCLDASRNGIRSLCDLPPSLVYLDVRDNQLIELPALPSGLERLIASFNHLAELPELPPNLYYLDASRNEISSLCDLPPSLVDLNVRKNQLIELPALPPDLERLIASFNHLAELPELPPNLSYLDASRNEISSLCDLPPSLVDLNVRKNQLIELPALPPDLERLIASFNHLAELPELPPNLSYLDASRNEISSLCDLPPSLVELDVRDNQLIELPALPPHLERLIASLNHLAEVPELPQNLKQLHVEHNALREFPDIPESVEDLRMD
proteinYersinia enterocolitica—34–481dimerMolecule count 250.321 kDa
DEAD box RNA helicase DDX3 (51-418)proteinYersinia enterocolitica—51–418monomerMolecule count 141.5 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11714[3]0.0767596–4.58324 1/nmHas uncertainty columnMissing 02sasbdb/entries/w8/sasdaw8/source/SASDAW8.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)15.01.5nm
i0Guinier7459.0——
mwExperimental123.012.0kDa
mwPorod119.012.0kDa
porod_volumePorod189.7—nm³
rgGuinier3.970.12nm
rgP(r)4.033—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name50 mM HEPES 150 mM NaClBuffer concentration50.0 mM
pH7.5Additives150 mM NaCl
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuterationFalse / —

Acquisition Conditions and Instrument

Measurement date2013-06-14Storage / measurement temperature23.0 / 23.0
Exposure time0.05Frames20
Wavelength0.12Sample-to-detector distance3.1
SourceX-ray synchrotronDetectorPilatus 2M
Facility / beamlinePETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.077 – 4.583Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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full_entry_zip:source record and ZIP contents (6 items)
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:196 field values
Field pathRaw value
codeSASDAW8
statusPublished
type_of_curveExtrapolated to infinite dilution
angular_unit1/nm
project.titleImmunosuppressive Yersinia Effector YopM Binds DEAD Box Helicase DDX3 to Control Ribosomal S6 Kinase in the Nucleus of Host Cells.
project.publication.titleImmunosuppressive Yersinia Effector YopM Binds DEAD Box Helicase DDX3 to Control Ribosomal S6 Kinase in the Nucleus of Host Cells.
project.publication.author_listBerneking L, Schnapp M, Rumm A, Trasak C, Ruckdeschel K, Alawi M, Grundhoff A, Kikhney AG, Koch-Nolte F, Buck F, Perbandt M, Betzel C, Svergun DI, Hentschke M, Aepfelbacher M
project.publication.journalPLoS Pathog
project.publication.doi10.1371/journal.ppat.1005660
project.publication.pmid27300509
project.publication.published_date2016 Jun
project.statusreleased
project.submitted_date2015-07-06
project.released_date2016-06-14
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameYersinia outer protein M (34-481)
experiment.sample.molecule[0].short_nameYopM
experiment.sample.molecule[0].sequenceKSKTEYYNAWSEWERNAPPGNGEQREMAVSRLRDCLDRQAHELELNNLGLSSLPELPPHLERLVASCNSLTELPELPQSL KSLEVYENNLKALPDLPPLLVDLRVFNNQLEELPELQNLPFLTEIYANNNSLKTLPDLPPSLVDLNVRENYLTALPELPQ SLIFLDISDNILSGLSELPPNLSCLDASRNGIRSLCDLPPSLVYLDVRDNQLIELPALPSGLERLIASFNHLAELPELPP NLYYLDASRNEISSLCDLPPSLVDLNVRKNQLIELPALPPDLERLIASFNHLAELPELPPNLSYLDASRNEISSLCDLPP SLVDLNVRKNQLIELPALPPDLERLIASFNHLAELPELPPNLSYLDASRNEISSLCDLPPSLVELDVRDNQLIELPALPP HLERLIASLNHLAEVPELPQNLKQLHVEHNALREFPDIPESVEDLRMD
experiment.sample.molecule[0].organismYersinia enterocolitica
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_first34
experiment.sample.molecule[0].uniprot_range_last481
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw50.321
experiment.sample.molecule[0].total_mw100.643
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_nameDEAD box RNA helicase DDX3 (51-418)
experiment.sample.molecule[1].short_nameDDX3
experiment.sample.molecule[1].sequencenull
experiment.sample.molecule[1].organismYersinia enterocolitica
experiment.sample.molecule[1].uniprot_codenull
experiment.sample.molecule[1].uniprot_range_first51
experiment.sample.molecule[1].uniprot_range_last418
experiment.sample.molecule[1].oligomerizationmonomer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequencenull
experiment.sample.molecule[1].mw41.5
experiment.sample.molecule[1].total_mw41.5
experiment.sample.molecule[1].number_molecules1
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
experiment.sample.buffer.name50 mM HEPES 150 mM NaCl
experiment.sample.buffer.concentration_unitmM
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive150 mM NaCl
experiment.sample.buffer.concentration50.0
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.name2:1 YopM:DDX3 complex
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixtureFalse
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameAl
experiment.contributor[0].contributor_surnameKikhney
experiment.contributor[0].orcidhttps://orcid.org/0000-0003-1321-3956
experiment.concentration_methodnull
experiment.concentration_unitmg/ml
experiment.date2013-06-14
experiment.storage_temperature23.0
experiment.cell_temperature23.0
experiment.exposure_time0.05
experiment.number_of_frames20
experiment.wavelength0.12
experiment.sample_detector_distance3.1
experiment.concentration_min0.66
experiment.concentration_max10.6
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.077
experiment.s_max4.583
experiment.total_exposure_time1.0
experiment.seccolumnnull
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fits[1].p_value0.145237
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fits[1].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionA complex composed of two YopM_34-481 molecules and one DDX3_51-418
experiment_descriptionnull
tags[]
intensity_unitnull
experimental_mw123.0
experimental_mw_error12.0
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw119.0
porod_mw_error12.0
pddf_i0null
pddf_i0_errornull
guinier_i07459.0
guinier_i0_errornull
pddf_rg4.033
pddf_rg_errornull
guinier_rg3.97
guinier_rg_error0.12
pddf_dmax15.0
pddf_dmax_error1.5
porod_volume189.7
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first15
guinier_point_last95
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDAW8_datloglog_img.png
symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDAW8
statussuccess
started_at2026-08-11T16:02:05.626113+00:00
finished_at2026-08-11T16:02:15.271321+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
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View the complete summary.json source
{
  "code": "SASDAW8",
  "status": "Published",
  "type_of_curve": "Extrapolated to infinite dilution",
  "angular_unit": "1/nm",
  "project": {
    "title": "Immunosuppressive Yersinia Effector YopM Binds DEAD Box Helicase DDX3 to Control Ribosomal S6 Kinase in the Nucleus of Host Cells.",
    "publication": {
      "title": "Immunosuppressive Yersinia Effector YopM Binds DEAD Box Helicase DDX3 to Control Ribosomal S6 Kinase in the Nucleus of Host Cells.",
      "author_list": "Berneking L, Schnapp M, Rumm A, Trasak C, Ruckdeschel K, Alawi M, Grundhoff A, Kikhney AG, Koch-Nolte F, Buck F, Perbandt M, Betzel C, Svergun DI, Hentschke M, Aepfelbacher M",
      "journal": "PLoS Pathog",
      "doi": "10.1371/journal.ppat.1005660",
      "pmid": "27300509",
      "published_date": "2016 Jun"
    },
    "status": "released",
    "submitted_date": "2015-07-06",
    "released_date": "2016-06-14"
  },
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        "resolution": null
      },
      "name": "PETRA III",
      "city": "DESY; Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
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