SASDB58

Major viral transcription factor ICP4 (ICP4N dimer)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2019-12-06T13:07:15.624532+01:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Major viral transcription factor ICP4 (ICP4N dimer)

浓度— – — 缓冲液 / pH20 mM HEPES, 150 mM NaCl / 7.4
Experimental temperature20.0 设备 / 束线Diamond Light Source / B21
波长0.1 nm曝光10.0 s × 17

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
Major viral transcription factor ICP4
查看序列
PAASAGRIERRRARAAVAGRDATGRFTAGQPRRVELDADATSGAFYARYRDGYVSGEPWPGAGPPPPGRVLYGGLGDSRPGLWGAPEAEEARRRFEASGAPAAVWAPELGDAAQQYALITRLLYTPDAEAMGWLQNPRVVPGDVALDQACFRISGAARNSSSFITGSVARAVPHLGYAMAAGRFGWGLAHAAAAVAMSRRYDRAQKGFLLTSLRRAYAPLLARENAALTG
proteinHuman alphaherpesvirus 1P08392258–487dimer分子数 224.31 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
1784[4]0.0352771–0.277713 1/A含误差列缺失 00sasbdb/entries/58/sasdb58/source/SASDB58.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)12.7Å
i0Guinier93.30.201
i0P(r)89.2
mwExperimental50.7kDa
rgGuinier2.880.022Å
rgP(r)3.07Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM HEPES, 150 mM NaCl缓冲液浓度
pH7.4添加剂
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2015-07-27储存 / 测量温度20.0 / 20.0
曝光时间10.0帧数17
波长0.1样品-探测器距离3.9
光源X-ray synchrotron探测器Pilatus 2M
机构 / 束线Diamond Light Source / B21 · Didcot, UK
q range0.353 – 2.777样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
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pddfsasbdb/entries/58/sasdb58/source/SASDB58.outdownloaded57023de092e2b3e068e9624d848f1948c5962fd32194a431388f837bb0ae0f78acc3Download查看原文件源站
sascifsasbdb/entries/58/sasdb58/source/SASDB58.sascifnot_availableDownload查看原文件源站
summarysasbdb/entries/58/sasdb58/source/summary.jsondownloaded7449d321c3a1e3df4e862f86d84eb8282393d344d61f1a2af37c391f57e9a0dbbb1dDownload查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:134 个字段值
字段路径原始值
codeSASDB58
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleThe herpes viral transcription factor ICP4 forms a novel DNA recognition complex.
project.publication.titleThe herpes viral transcription factor ICP4 forms a novel DNA recognition complex.
project.publication.author_listTunnicliffe RB, Lockhart-Cairns MP, Levy C, Mould AP, Jowitt TA, Sito H, Baldock C, Sandri-Goldin RM, Golovanov AP
project.publication.journalNucleic Acids Res
project.publication.doi10.1093/nar/gkx419
project.publication.pmid28505309
project.publication.published_date2017 Jul 27
project.statusreleased
project.submitted_date2016-11-28
project.released_date2017-05-30
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDB58.dat
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDB58.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB58_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB58_kratky_img.png
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intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB58_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDB58.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.nameDiamond Light Source
experiment.instrument.cityDidcot
experiment.instrument.countryUK
experiment.instrument.beamline_nameB21
experiment.instrument.beam_geometry1 x 5 mm
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameMajor viral transcription factor ICP4
experiment.sample.molecule[0].short_nameICP4N
experiment.sample.molecule[0].sequencePAASAGRIERRRARAAVAGRDATGRFTAGQPRRVELDADATSG AFYARYRDGYVSGEPWPGAGPPPPGRVLYGGLGDSRPGLWGAPEAEEARRRFEASGAPAA VWAPELGDAAQQYALITRLLYTPDAEAMGWLQNPRVVPGDVALDQACFRISGAARNSSSF ITGSVARAVPHLGYAMAAGRFGWGLAHAAAAVAMSRRYDRAQKGFLLTSLRRAYAPLLAR ENAALTG
experiment.sample.molecule[0].organismHuman alphaherpesvirus 1
experiment.sample.molecule[0].uniprot_codeP08392
experiment.sample.molecule[0].uniprot_range_first258
experiment.sample.molecule[0].uniprot_range_last487
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMASENKQRPGSPGPTDGPPPTPSPDRDERGALGWGAETEEGGDDPDHDPDHPHDLDDARR DGRAPAAGTDAGEDAGDAVSPRQLALLASMVEEAVRTIPTPDPAASPPRTPAFRADDDDG DEYDDAADAAGDRAPARGREREAPLRGAYPDPTDRLSPRPPAQPPRRRRHGRWRPSASST SSDSGSSSSSSASSSSSSSDEDEDDDGNDAADHAREARAVGRGPSSAAPAAPGRTPPPPG PPPLSEAAPKPRAAARTPAASAGRIERRRARAAVAGRDATGRFTAGQPRRVELDADATSG AFYARYRDGYVSGEPWPGAGPPPPGRVLYGGLGDSRPGLWGAPEAEEARRRFEASGAPAA VWAPELGDAAQQYALITRLLYTPDAEAMGWLQNPRVVPGDVALDQACFRISGAARNSSSF ITGSVARAVPHLGYAMAAGRFGWGLAHAAAAVAMSRRYDRAQKGFLLTSLRRAYAPLLAR ENAALTGAAGSPGAGADDEGVAAVAAAAPGERAVPAGYGAAGILAALGRLSAAPASPAGG DDPDAARHADADDDAGRRAQAGRVAVECLAACRGILEALAEGFDGDLAAVPGLAGARPAS PPRPEGPAGPASPPPPHADAPRLRAWLRELRFVRDALVLMRLRGDLRVAGGSEAAVAAVR AVSLVAGALGPALPRDPRLPSSAAAAAADLLFDNQSLRPLLAAAASAPDAADALAAAAAS AAPREGRKRKSPGPARPPGGGGPRPPKTKKSGADAPGSDARAPLPAPAPPSTPPGPEPAP AQPAAPRAAAAQARPRPVAVSRRPAEGPDPLGGWRRQPPGPSHTAAPAAAALEAYCSPRA VAELTDHPLFPVPWRPALMFDPRALASIAARCAGPAPAAQAACGGGDDDDNPHPHGAAGG RLFGPLRASGPLRRMAAWMRQIPDPEDVRVVVLYSPLPGEDLAGGGASGGPPEWSAERGG LSCLLAALANRLCGPDTAAWAGNWTGAPDVSALGAQGVLLLSTRDLAFAGAVEFLGLLAS AGDRRLIVVNTVRACDWPADGPAVSRQHAYLACELLPAVQCAVRWPAARDLRRTVLASGR VFGPGVFARVEAAHARLYPDAPPLRLCRGGNVRYRVRTRFGPDTPVPMSPREYRRAVLPA LDGRAAASGTTDAMAPGAPDFCEEEAHSHAACARWGLGAPLRPVYVALGREAVRAGPARW RGPRRDFCARALLEPDDDAPPLVLRGDDDGPGALPPAPPGIRWASATGRSGTVLAAAGAV EVLGAEAGLATPPRREVVDWEGAWDEDDGGAFEGDGVL
experiment.sample.molecule[0].mw24.31
experiment.sample.molecule[0].total_mw48.62
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM HEPES, 150 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameMajor viral transcription factor ICP4 (ICP4N dimer)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressManchester, UK
experiment.contributor[0].affiliation[0].full_nameUniversity of Manchester
experiment.contributor[0].affiliation[0].webpagehttp://www.manchester.ac.uk/
experiment.contributor[0].contributor_nameMichael
experiment.contributor[0].contributor_surnameLockhart
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2015-07-27
experiment.storage_temperature20.0
experiment.cell_temperature20.0
experiment.exposure_time10.0
experiment.number_of_frames17
experiment.wavelength0.1
experiment.sample_detector_distance3.9
experiment.concentration_minnull
experiment.concentration_maxnull
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.353
experiment.s_max2.777
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareScÅtter
pddf_software_versionScÅtter 3.0g
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of Major viral transcription factor ICP4 (ICP4N dimer) in 20 mM HEPES, 150 mM NaCl, pH 7.4, were collected at the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 3.9 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). Data were collected at 20°C using 17 successive 10 second frames at an undetermined sample concentration. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
intensity_unitnull
experimental_mw50.7
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i089.2
pddf_i0_errornull
guinier_i093.3
guinier_i0_error0.201
pddf_rg3.07
pddf_rg_errornull
guinier_rg2.88
guinier_rg_error0.022
pddf_dmax12.7
pddf_dmax_errornull
porod_volumenull
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last27
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDB58_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDB58
statussuccess
started_at2026-08-11T16:33:08.824612+00:00
finished_at2026-08-11T16:33:15.769563+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
{
  "code": "SASDB58",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "The herpes viral transcription factor ICP4 forms a novel DNA recognition complex.",
    "publication": {
      "title": "The herpes viral transcription factor ICP4 forms a novel DNA recognition complex.",
      "author_list": "Tunnicliffe RB, Lockhart-Cairns MP, Levy C, Mould AP, Jowitt TA, Sito H, Baldock C, Sandri-Goldin RM, Golovanov AP",
      "journal": "Nucleic Acids Res",
      "doi": "10.1093/nar/gkx419",
      "pmid": "28505309",
      "published_date": "2017 Jul 27"
    },
    "status": "released",
    "submitted_date": "2016-11-28",
    "released_date": "2017-05-30"
  },
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  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDB58.sascif",
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      "type_of_source": "X-ray synchrotron",
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      "xray_energy": null,
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    "sample": {
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          "long_name": "Major viral transcription factor ICP4",
          "short_name": "ICP4N",
          "sequence": "PAASAGRIERRRARAAVAGRDATGRFTAGQPRRVELDADATSG\r\nAFYARYRDGYVSGEPWPGAGPPPPGRVLYGGLGDSRPGLWGAPEAEEARRRFEASGAPAA\r\nVWAPELGDAAQQYALITRLLYTPDAEAMGWLQNPRVVPGDVALDQACFRISGAARNSSSF\r\nITGSVARAVPHLGYAMAAGRFGWGLAHAAAAVAMSRRYDRAQKGFLLTSLRRAYAPLLAR\r\nENAALTG",
          "organism": "Human alphaherpesvirus 1",
          "uniprot_code": "P08392",
          "uniprot_range_first": 258,
          "uniprot_range_last": 487,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MASENKQRPGSPGPTDGPPPTPSPDRDERGALGWGAETEEGGDDPDHDPDHPHDLDDARR\nDGRAPAAGTDAGEDAGDAVSPRQLALLASMVEEAVRTIPTPDPAASPPRTPAFRADDDDG\nDEYDDAADAAGDRAPARGREREAPLRGAYPDPTDRLSPRPPAQPPRRRRHGRWRPSASST\nSSDSGSSSSSSASSSSSSSDEDEDDDGNDAADHAREARAVGRGPSSAAPAAPGRTPPPPG\nPPPLSEAAPKPRAAARTPAASAGRIERRRARAAVAGRDATGRFTAGQPRRVELDADATSG\nAFYARYRDGYVSGEPWPGAGPPPPGRVLYGGLGDSRPGLWGAPEAEEARRRFEASGAPAA\nVWAPELGDAAQQYALITRLLYTPDAEAMGWLQNPRVVPGDVALDQACFRISGAARNSSSF\nITGSVARAVPHLGYAMAAGRFGWGLAHAAAAVAMSRRYDRAQKGFLLTSLRRAYAPLLAR\nENAALTGAAGSPGAGADDEGVAAVAAAAPGERAVPAGYGAAGILAALGRLSAAPASPAGG\nDDPDAARHADADDDAGRRAQAGRVAVECLAACRGILEALAEGFDGDLAAVPGLAGARPAS\nPPRPEGPAGPASPPPPHADAPRLRAWLRELRFVRDALVLMRLRGDLRVAGGSEAAVAAVR\nAVSLVAGALGPALPRDPRLPSSAAAAAADLLFDNQSLRPLLAAAASAPDAADALAAAAAS\nAAPREGRKRKSPGPARPPGGGGPRPPKTKKSGADAPGSDARAPLPAPAPPSTPPGPEPAP\nAQPAAPRAAAAQARPRPVAVSRRPAEGPDPLGGWRRQPPGPSHTAAPAAAALEAYCSPRA\nVAELTDHPLFPVPWRPALMFDPRALASIAARCAGPAPAAQAACGGGDDDDNPHPHGAAGG\nRLFGPLRASGPLRRMAAWMRQIPDPEDVRVVVLYSPLPGEDLAGGGASGGPPEWSAERGG\nLSCLLAALANRLCGPDTAAWAGNWTGAPDVSALGAQGVLLLSTRDLAFAGAVEFLGLLAS\nAGDRRLIVVNTVRACDWPADGPAVSRQHAYLACELLPAVQCAVRWPAARDLRRTVLASGR\nVFGPGVFARVEAAHARLYPDAPPLRLCRGGNVRYRVRTRFGPDTPVPMSPREYRRAVLPA\nLDGRAAASGTTDAMAPGAPDFCEEEAHSHAACARWGLGAPLRPVYVALGREAVRAGPARW\nRGPRRDFCARALLEPDDDAPPLVLRGDDDGPGALPPAPPGIRWASATGRSGTVLAAAGAV\nEVLGAEAGLATPPRREVVDWEGAWDEDDGGAFEGDGVL",
          "mw": 24.31,
          "total_mw": 48.62,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
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        "name": "20 mM HEPES, 150 mM NaCl",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.4,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Major viral transcription factor ICP4 (ICP4N dimer)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Manchester, UK",
            "full_name": "University of Manchester",
            "webpage": "http://www.manchester.ac.uk/"
          }
        ],
        "contributor_name": "Michael",
        "contributor_surname": "Lockhart",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2015-07-27",
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    "cell_temperature": 20.0,
    "exposure_time": 10.0,
    "number_of_frames": 17,
    "wavelength": 0.1,
    "sample_detector_distance": 3.9,
    "concentration_min": null,
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    "flow_rate": null,
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    "s_max": 2.777,
    "total_exposure_time": null,
    "seccolumn": null
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  "fits": [],
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  "pddf_software": "ScÅtter",
  "pddf_software_version": "ScÅtter 3.0g",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of Major viral transcription factor ICP4 (ICP4N dimer) in 20 mM HEPES, 150 mM NaCl, pH 7.4, were collected at the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 3.9 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). Data were collected at 20°C using 17 successive 10 second frames at an undetermined sample concentration. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
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  "pddf_i0": 89.2,
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  "pddf_rg": 3.07,
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  "pddf_dmax": 12.7,
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  "estimated_volume": null,
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  "guinier_point_first": 1,
  "guinier_point_last": 27,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDB58_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
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