Leishmania braziliensis stress-induced protein sti1 (LbHop TPR2A-TPR2B-DP2 construct)

{
  "code": "SASDB64",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/nm",
  "project": {
    "title": "Low sequence identity but high structural and functional conservation: The case of Hsp70/Hsp90 organizing protein (Hop/Sti1) of Leishmania braziliensis.",
    "publication": {
      "title": "Low sequence identity but high structural and functional conservation: The case of Hsp70/Hsp90 organizing protein (Hop/Sti1) of Leishmania braziliensis.",
      "author_list": "Batista FAH, Seraphim TV, Santos CA, Gonzaga MR, Barbosa LRS, Ramos CHI, Borges JC",
      "journal": "Arch Biochem Biophys",
      "doi": "10.1016/j.abb.2016.04.008",
      "pmid": "27103305",
      "published_date": "2016 Jun 15"
    },
    "status": "released",
    "submitted_date": "2016-02-21",
    "released_date": "2016-08-01"
  },
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  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDB64.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB64_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB64_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDB64_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB64_guinier_img.png",
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  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "MAR 165 CCD",
        "resolution": null
      },
      "name": "Brazilian Synchrotron Light Laboratory",
      "city": "Campinas",
      "country": "Brazil",
      "beamline_name": "SAXS2 Beamline",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
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      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Stress-induced protein sti1 (Hop TPR2A-TPR2B-DP2 construct)",
          "short_name": "Truncated Hop/Sti1",
          "sequence": "GSHMEDQRFALTLMYLSGMKIPNDDEDDEEERPSAKAAAEAKAKEEKKLLTDNEKEAMALKEEGNKLYLSKRFEEALSKYQEAQAKDPKNTLYILNVSAVYFEQRDYEKCITECERGIEHGRENHCDYTIVAKLMTRHAFCLQKQKKYEAAIDLYKRALVEWRNPDTLKKLTECEKEHQKAVEEAYIDPEIARQKKDEGNQYFKEDKFPEAVAAYTEAIKRNPAEHTSYSNRAAAYIKLGAFNDALKDAEKCIELKPDFVKGYARKGHAYFWTKQYNRALQAYDEGLKVDPSNADCKDGRLRTIMRIQEMASGQSADGDEAARRAMDDPEIAAIMQDSYMQLVLKEMQNDPTRIQEYMKDPGISVKINKLISAGIIRFGQ",
          "organism": "Leishmania braziliensis",
          "uniprot_code": "A4H5F0",
          "uniprot_range_first": 171,
          "uniprot_range_last": 547,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MDANELKNEGNKEFSAGRYVEAVNYFSKAIQLDGQNSVLYSNRSACFAAMQKYKDALDDA\nDKCISIKPNWAKGYVRRGAALHGMRRYDDAIAAYEKGLSVDPSNSGCTQGVKDVQVAKSR\nEARDPIARVFTPEAFRKIQENPKLSLLMLQPDYVKMVDTVVRDPSQARLYMEDQRFALTL\nMYLSGMKIPNDDEDDEEERPSAKAAAEAKAKEEKKLLTDNEKEAMALKEEGNKLYLSKRF\nEEALSKYQEAQAKDPKNTLYILNVSAVYFEQRDYEKCITECERGIEHGRENHCDYTIVAK\nLMTRHAFCLQKQKKYEAAIDLYKRALVEWRNPDTLKKLTECEKEHQKAVEEAYIDPEIAR\nQKKDEGNQYFKEDKFPEAVAAYTEAIKRNPAEHTSYSNRAAAYIKLGAFNDALKDAEKCI\nELKPDFVKGYARKGHAYFWTKQYNRALQAYDEGLKVDPSNADCKDGRLRTIMRIQEMASG\nQSADGDEAARRAMDDPEIAAIMQDSYMQLVLKEMQNDPTRIQEYMKDPGISVKINKLISA\nGIIRFGQ",
          "mw": 43.7,
          "total_mw": 43.7,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "25 mM Tris 100 mM NaCl 1 mM EDTA 1 mM β-mercaptoethanol",
        "concentration_unit": "mM",
        "comment": null,
        "additive": "100 mM NaCl, 1 mM EDTA, 1 mM β-mercaptoethanol",
        "concentration": 25.0,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Leishmania braziliensis stress-induced protein sti1 (LbHop TPR2A-TPR2B-DP2 construct)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "São Carlos - SP - Brasil",
            "full_name": "Instituto de Química de São Carlos",
            "webpage": ""
          }
        ],
        "contributor_name": "Júlio",
        "contributor_surname": "Borges",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": "mg/ml",
    "date": "2016-02-21",
    "storage_temperature": null,
    "cell_temperature": null,
    "exposure_time": null,
    "number_of_frames": null,
    "wavelength": null,
    "sample_detector_distance": null,
    "concentration_min": 1.4,
    "concentration_max": 8.5,
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    "s_min": 0.184,
    "s_max": 3.181,
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          "user": 100
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      "software": null,
      "chi_square_value": 1.749,
      "p_value": 0.203609,
      "fit_residual_plot": "SASDB64_fit1_fitresiduals_img.png",
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  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": null,
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "X-ray synchrotron radiation scattering data from solutions of stress-induced protein sti1 (Hop/Sti1), amino acids 171-547 (N-terminal truncation, Hop TPR2A-TPR2B-DP2 construct) in 25 mM Tris 100 mM NaCl, 1 mM EDTA, 1 mM β-mercaptoethanol were collected on the SAXS2 beam line at the Brazilian Synchrotron Light Laboratory (Campinas, Brazil) using a MAR Image Plate detector (s = 4π sin θ/λ, where 2θ is the scattering angle). Different solute concentrations in the range 1.40-8.50 mg/ml were measured. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected were merged to yield the final composite scattering curve.",
  "tags": [],
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  "experimental_mw_error": null,
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  "porod_mw_error": null,
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  "guinier_point_first": 2,
  "guinier_point_last": 13,
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  "last_modified": "2019-12-06T13:07:15.624532+01:00",
  "bragg_peak": []
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