Immediate Early 3 DNA (ICP4N IE3_19mer)

{
  "code": "SASDB68",
  "status": "Published",
  "type_of_curve": "Other",
  "angular_unit": "1/A",
  "project": {
    "title": "The herpes viral transcription factor ICP4 forms a novel DNA recognition complex.",
    "publication": {
      "title": "The herpes viral transcription factor ICP4 forms a novel DNA recognition complex.",
      "author_list": "Tunnicliffe RB, Lockhart-Cairns MP, Levy C, Mould AP, Jowitt TA, Sito H, Baldock C, Sandri-Goldin RM, Golovanov AP",
      "journal": "Nucleic Acids Res",
      "doi": "10.1093/nar/gkx419",
      "pmid": "28505309",
      "published_date": "2017 Jul 27"
    },
    "status": "released",
    "submitted_date": "2016-11-28",
    "released_date": "2017-05-30"
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  "experiment": {
    "instrument": {
      "detector": {
        "type": "Dectris",
        "name": "Pilatus 1M",
        "resolution": 172.0
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
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      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Immediate Early 3",
          "short_name": "IE3",
          "sequence": "CCGATCGTCCACACGGAGC",
          "organism": null,
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "dimer",
          "molecular_type": "DNA",
          "uniprot_sequence": null,
          "mw": 5.839,
          "total_mw": 11.678,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "20 mM HEPES, 150 mM NaCl",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.4,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Immediate Early 3 DNA (ICP4N IE3_19mer)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Manchester, UK",
            "full_name": "University of Manchester",
            "webpage": "http://www.manchester.ac.uk/"
          }
        ],
        "contributor_name": "Michael",
        "contributor_surname": "Lockhart",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2015-11-12",
    "storage_temperature": 20.0,
    "cell_temperature": 20.0,
    "exposure_time": 1.0,
    "number_of_frames": null,
    "wavelength": 0.0992,
    "sample_detector_distance": 2.9,
    "concentration_min": null,
    "concentration_max": null,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.224,
    "s_max": 3.396,
    "total_exposure_time": null,
    "seccolumn": null
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  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ScÅtter",
  "pddf_software_version": "3.0g",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of Immediate Early 3 DNA (ICP4N IE3_19mer) in 20 mM HEPES, 150 mM NaCl, pH 7.4, were collected using HPLC SEC-SAXS at the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.0992 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). The sample was separated using a Superdex 200 increase 3.2/300 size exclusion chromatography column before exposure to X-rays. 50 µL of IE3_19merwas loaded onto the Superdex column and the eluent was flowed through the SAXS beam at 0.075 mL/min; the buffer used as the background was collected after one SEC column volume. Data were collected at 20°C at one second intervals.",
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  "experimental_mw": 0.0,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": null,
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  "pddf_i0_error": null,
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  "guinier_i0_error": 0.048,
  "pddf_rg": 1.925,
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  "guinier_rg_error": 0.011,
  "pddf_dmax": 6.9,
  "pddf_dmax_error": null,
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  "estimated_volume_error": null,
  "guinier_point_first": 1,
  "guinier_point_last": 34,
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  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
  "bragg_peak": []
}

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