E244K monomeric human Properdin

{
  "code": "SASDB69",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Functional and structural insight into properdin control of complement alternative pathway amplification.",
    "publication": {
      "title": "Functional and structural insight into properdin control of complement alternative pathway amplification.",
      "author_list": "Pedersen DV, Roumenina L, Jensen RK, Gadeberg TA, Marinozzi C, Picard C, Rybkine T, Thiel S, Sørensen UB, Stover C, Fremeaux-Bacchi V, Andersen GR",
      "journal": "EMBO J",
      "doi": "10.15252/embj.201696173",
      "pmid": "28264884",
      "published_date": "2017 Apr 13"
    },
    "status": "released",
    "submitted_date": "2017-01-02",
    "released_date": "2017-03-12"
  },
  "pddf_data": null,
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDB69.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB69_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB69_kratky_img.png",
  "pddf_plot": null,
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDB69_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDB69.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus 2M",
        "resolution": null
      },
      "name": "PETRA III",
      "city": "DESY; Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "E244K Human Properdin",
          "short_name": "E244K FP",
          "sequence": "DPVLCFTQYEESSGKCKGLLGGGVSVEDCCLNTAFAYQKRSGGLCQPCRSPRWSLWSTWA\r\nPCSVTCSEGSQLRYRRCVGWNGQCSGKVAPGTLEWQLQACEDQQCCPEMGGWSGWGPWEP\r\nCSVTCSKGTRTRRRACNHPAPKCGGHCPGQAQESEACDTQQVCPTHGAWATWGPWTPCSA\r\nSCHGGPHEPKETRSRKCSAPEPSQKPPGKPCPGLAYKQRRCTGLPPCPENLYFQGVAGGW\r\nGPWGPVSPCPVTCGLGQTMEQRTCNHPVPQHGGPFCAGDATRTHICNTAVPCPVDGEWDS\r\nWGEWSPCIRRNMKSISCQEIPGQQSRGRTCRGRKFDGHRCAGQQQDIRHCYSIQHCPLKG\r\nSWSEWSTWGLCMPPCGPNPTRARQRLCTPLLPKYPPTVSMVEGQGEKNVTFWGRPLPRCE\r\nELQGQKLVVEEKRPCLHVPACKDPEEEEL",
          "organism": "Homo sapiens",
          "uniprot_code": "P27918",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MITEGAQAPRLLLPPLLLLLTLPATGSDPVLCFTQYEESSGKCKGLLGGGVSVEDCCLNT\nAFAYQKRSGGLCQPCRSPRWSLWSTWAPCSVTCSEGSQLRYRRCVGWNGQCSGKVAPGTL\nEWQLQACEDQQCCPEMGGWSGWGPWEPCSVTCSKGTRTRRRACNHPAPKCGGHCPGQAQE\nSEACDTQQVCPTHGAWATWGPWTPCSASCHGGPHEPKETRSRKCSAPEPSQKPPGKPCPG\nLAYEQRRCTGLPPCPVAGGWGPWGPVSPCPVTCGLGQTMEQRTCNHPVPQHGGPFCAGDA\nTRTHICNTAVPCPVDGEWDSWGEWSPCIRRNMKSISCQEIPGQQSRGRTCRGRKFDGHRC\nAGQQQDIRHCYSIQHCPLKGSWSEWSTWGLCMPPCGPNPTRARQRLCTPLLPKYPPTVSM\nVEGQGEKNVTFWGRPLPRCEELQGQKLVVEEKRPCLHVPACKDPEEEEL",
          "mw": 49.345,
          "total_mw": 49.345,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "10 mM HEPES 150 mM NaCl",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.2,
        "deuteration": null
      },
      "purity_method": null,
      "name": "E244K monomeric human Properdin",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Aarhus, Denmark",
            "full_name": "Aarhus University",
            "webpage": "http://www.au.dk"
          }
        ],
        "contributor_name": "Rasmus",
        "contributor_surname": "Jensen",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2016-07-27",
    "storage_temperature": 20.0,
    "cell_temperature": 20.0,
    "exposure_time": 0.05,
    "number_of_frames": 20,
    "wavelength": 0.124,
    "sample_detector_distance": 3.0,
    "concentration_min": null,
    "concentration_max": 2.7,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.154,
    "s_max": 2.799,
    "total_exposure_time": null,
    "seccolumn": null
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          "pdb_link": [],
          "model_title": null,
          "type_of_model": "atomic",
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          "model_mw": 53.64,
          "bead_radius": null,
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          "comment": null,
          "user": 185
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      ],
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      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDB69_fit1_fixed_fit_img.png",
      "software": "CRYSOL",
      "chi_square_value": 1.61,
      "p_value": 0.0,
      "fit_residual_plot": "SASDB69_fit1_fitresiduals_img.png",
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      "fit_log": null,
      "software_version": "Crysol3",
      "description": ""
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  "pddf_software_version": null,
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  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of recombinantly formed monomeric human E244K properdin in 10 mM HEPES pH 7.2, 150 mM NaCl, were collected EMBL-P12 bioSAXS beam line at the PETRAIII storage ring (Hamburg, Germany) equipped with a Pilatus 2M detector (I(s) vs s, where s = 4π sin θ/λ; 2θ is the scattering angle; λ = 0.124 nm). A solute concentration of 2.7 mg/ml was measured at 20°C. 20 successive 0.05 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and scaled for protein concentration.",
  "tags": [],
  "intensity_unit": null,
  "experimental_mw": 50.0,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": null,
  "porod_mw_error": null,
  "pddf_i0": null,
  "pddf_i0_error": null,
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  "guinier_i0_error": 67.36,
  "pddf_rg": null,
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  "guinier_rg_error": 0.04,
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  "pddf_dmax_error": null,
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  "porod_volume_error": null,
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  "estimated_volume_error": null,
  "guinier_point_first": 5,
  "guinier_point_last": 59,
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  "symmetry": null,
  "last_modified": "2023-06-21T07:56:39.492710+02:00",
  "bragg_peak": []
}

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