SASDB89

Telomeric DNA duplex

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2019-12-06T13:07:15.624532+01:00

Refer to the related SASBDB entries SASDB79 and SASDC62.

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Telomeric DNA duplex

浓度— – 1.4 缓冲液 / pH20 mM Tris-HCl, 50 mM LiCl / 7.5
Experimental temperature4.0 设备 / 束线CEITEC / Rigaku BioSAXS-1000
波长0.154 nm曝光60.0 s × 6

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
telomere DNA duplex
查看序列
GTTAGGGTTAGGGTTAGCAATCCCAATCCCAATC
DNA—–—monomer分子数 110.522 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
1186[3]0.0339619–0.300378 1/A含误差列缺失 00sasbdb/entries/89/sasdb89/source/SASDB89.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)5.71Å
i0Guinier0.0940.001
i0P(r)0.097530.001077
mwExperimental10.5kDa
porod_volumePorod11.9ų
rgGuinier1.5880.035Å
rgP(r)1.7090.024Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM Tris-HCl, 50 mM LiCl缓冲液浓度
pH7.5添加剂
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2016-05-03储存 / 测量温度4.0 / 4.0
曝光时间60.0帧数6
波长0.154样品-探测器距离0.4791
光源X-ray in house探测器Pilatus 100K
机构 / 束线CEITEC / Rigaku BioSAXS-1000 · Brno, Czech Republic
q range0.34 – 3.004样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
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summarysasbdb/entries/89/sasdb89/source/summary.jsondownloaded7034ec6ba447369f9e22370622e95e4411304c79b8be5eb77de195ca954c55081c93Download查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDB89
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleBasic domain of telomere guardian TRF2 reduces D-loop unwinding whereas Rap1 restores it.
project.publication.titleBasic domain of telomere guardian TRF2 reduces D-loop unwinding whereas Rap1 restores it.
project.publication.author_listNecasová I, Janoušková E, Klumpler T, Hofr C
project.publication.journalNucleic Acids Res
project.publication.doi10.1093/nar/gkx812
project.publication.pmid28981702
project.publication.published_date2017 Dec 1
project.statusreleased
project.submitted_date2017-01-06
project.released_date2017-09-18
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 100K
experiment.instrument.detector.resolution172.0
experiment.instrument.nameCEITEC
experiment.instrument.cityBrno
experiment.instrument.countryCzech Republic
experiment.instrument.beamline_nameRigaku BioSAXS-1000
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray in house
experiment.instrument.point_sourcenull
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experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceGTTAGGGTTAGGGTTAG CAATCCCAATCCCAATC
experiment.sample.molecule[0].organismnull
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeDNA
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw10.522
experiment.sample.molecule[0].total_mw10.522
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM Tris-HCl, 50 mM LiCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameTelomeric DNA duplex
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
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experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
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experiment.contributor[0].affiliation[0].webpagehttps://www.ceitec.eu/
experiment.contributor[0].contributor_nameTomas
experiment.contributor[0].contributor_surnameKlumpler
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2016-05-03
experiment.storage_temperature4.0
experiment.cell_temperature4.0
experiment.exposure_time60.0
experiment.number_of_frames6
experiment.wavelength0.154
experiment.sample_detector_distance0.4791
experiment.concentration_minnull
experiment.concentration_max1.4
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.34
experiment.s_max3.004
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionGNOM Version 5.0
i0_calibration_standardnull
descriptionRefer to the related SASBDB entries SASDB79 and SASDC62.
experiment_descriptionSAXS data from solutions of telomeric DNA duplex in 20 mM Tris-HCl, 50 mM LiCl, pH 7.5 were collected using a Rigaku BioSAXS-1000 instrument at CEITEC (Brno, Czech Republic) equipped with a Pilatus 100K detector at a sample-detector distance of 0.5 m (I(s) vs s, where = 4πsinθ/λ; 2θ is the scattering angle and λ = 0.154 nm). Six successive 3600 second frames were collected at a sample temperature of 4°C and a solute concentration of 1.4 mg/ml. The data were normalized to the intensity of the transmitted beam and radially averaged and the corresponding scattering from the solvent-blank was subtracted to produced the scattering profile displayed in this entry.
tags[]
intensity_unitnull
experimental_mw10.5
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i00.09753
pddf_i0_error0.001077
guinier_i00.094
guinier_i0_error0.001
pddf_rg1.709
pddf_rg_error0.024
guinier_rg1.588
guinier_rg_error0.035
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pddf_dmax_errornull
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porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first2
guinier_point_last34
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDB89_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDB89
statussuccess
started_at2026-08-11T16:08:12.235378+00:00
finished_at2026-08-11T16:08:19.711164+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
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  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Basic domain of telomere guardian TRF2 reduces D-loop unwinding whereas Rap1 restores it.",
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      "author_list": "Necasová I, Janoušková E, Klumpler T, Hofr C",
      "journal": "Nucleic Acids Res",
      "doi": "10.1093/nar/gkx812",
      "pmid": "28981702",
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  ],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "GNOM Version 5.0",
  "i0_calibration_standard": null,
  "description": "Refer to the related SASBDB entries SASDB79 and SASDC62.",
  "experiment_description": "SAXS data from solutions of telomeric DNA duplex in 20 mM Tris-HCl, 50 mM LiCl, pH 7.5 were collected using a Rigaku BioSAXS-1000 instrument at CEITEC (Brno, Czech Republic) equipped with a Pilatus 100K detector at a sample-detector distance of 0.5 m (I(s) vs s, where = 4πsinθ/λ; 2θ is the scattering angle and λ = 0.154 nm). Six successive 3600 second frames were collected at a sample temperature of 4°C and a solute concentration of 1.4 mg/ml. The data were normalized to the intensity of the transmitted beam and radially averaged and the corresponding scattering from the solvent-blank was subtracted to produced the scattering profile displayed in this entry.",
  "tags": [],
  "intensity_unit": null,
  "experimental_mw": 10.5,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": null,
  "porod_mw_error": null,
  "pddf_i0": 0.09753,
  "pddf_i0_error": 0.001077,
  "guinier_i0": 0.094,
  "guinier_i0_error": 0.001,
  "pddf_rg": 1.709,
  "pddf_rg_error": 0.024,
  "guinier_rg": 1.588,
  "guinier_rg_error": 0.035,
  "pddf_dmax": 5.71,
  "pddf_dmax_error": null,
  "porod_volume": 11.9,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 2,
  "guinier_point_last": 34,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDB89_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
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  "source_last_modified": "2019-12-06T13:07:15.624532+01:00",
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