SASDB94

Suppressor of Copper Sensitivity C protein (ScsC) from Proteus mirabilis

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2020-08-28T15:27:57.490687+02:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Suppressor of Copper Sensitivity C protein (ScsC) from Proteus mirabilis

浓度2.15 – 8.85 缓冲液 / pH25 mM HEPES 150mM NaCl 1mM DTT / 7.5
Experimental temperature10.0 设备 / 束线Australian Synchrotron / SAXS/WAXS
波长— nm曝光2.0 s × 14

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
C-terminal catalytic domain of Suppressor of Copper Sensitivity C protein
查看序列
SNAQFRQALASEHDALYNDAASPRIGAKDAKLVLVSFTDYNCPYCKRFDPLLEKITEQYPDVAVIIKPLPFKGESSAKASQAVLSVWKEDPKAFLALHQRLMQKKTMLDNASIEDAMKSTNTSKIKLTDDSLKTLQNNLELSRKLGIQGTPATVIGDTILPGAVDYDQLEIIVKEQLAKVKK
proteinProteus mirabilisA0A1Z1SYD564–243monomer分子数 120.15 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
1182[3]0.0862801–2.48729 1/nm含误差列缺失 00sasbdb/entries/94/sasdb94/source/SASDB94.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)10.5nm
i0Guinier0.101
i0P(r)0.10060.00016
mwExperimental69.9kDa
porod_volumePorod92.0nm³
rgGuinier3.65nm
rgP(r)3.60.01nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称25 mM HEPES 150mM NaCl 1mM DTT缓冲液浓度25.0 mM
pH7.5添加剂150mM NaCl, 1mM DTT
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2012-02-29储存 / 测量温度10.0 / 10.0
曝光时间2.0帧数14
波长样品-探测器距离1.6
光源X-ray synchrotron探测器Pilatus 1M
机构 / 束线Australian Synchrotron / SAXS/WAXS · Melbourne, Australia
q range0.086 – 2.487样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
curvesasbdb/entries/94/sasdb94/source/SASDB94.datdownloaded85168e69899230f6dd6ed541a0a8d076263b0cc7aab89f76ffc7b259cce1ea1d7cbaDownload查看原文件源站
full_entry_zipsasbdb/entries/94/sasdb94/source/SASDB94.zipdownloaded138659188c9a755eadaad41d1032127c1b70d7b47f35f8c3ca7bc6eca437eed4c030e48Download查看原文件源站
pddfsasbdb/entries/94/sasdb94/source/SASDB94.outdownloaded18248238e944063660afbf8880f1f2250ec9a4b61bcb73f44f6b6f551763a7291cb66Download查看原文件源站
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summarysasbdb/entries/94/sasdb94/source/summary.jsondownloaded116368167a2d1757095edb00c978d296562b4fdf53715456fbd470b56581882d16968Download查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(14 项)
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pddf:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:247 个字段值
字段路径原始值
codeSASDB94
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleA shape-shifting redox foldase contributes to Proteus mirabilis copper resistance.
project.publication.titleA shape-shifting redox foldase contributes to Proteus mirabilis copper resistance.
project.publication.author_listFurlong EJ, Lo AW, Kurth F, Premkumar L, Totsika M, Achard MES, Halili MA, Heras B, Whitten AE, Choudhury HG, Schembri MA, Martin JL
project.publication.journalNat Commun
project.publication.doi10.1038/ncomms16065
project.publication.pmid28722010
project.publication.published_date2017 Jul 19
project.statusreleased
project.submitted_date2016-10-04
project.released_date2017-06-16
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experiment.instrument.detector.typeDectris
experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameAustralian Synchrotron
experiment.instrument.cityMelbourne
experiment.instrument.countryAustralia
experiment.instrument.beamline_nameSAXS/WAXS
experiment.instrument.beam_geometryPoint
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourceTrue
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameC-terminal catalytic domain of Suppressor of Copper Sensitivity C protein
experiment.sample.molecule[0].short_namePmScsCΔN
experiment.sample.molecule[0].sequenceSNAQFRQALASEHDALYNDAASPRIGAKDAKLVLVSFTDYNCPYCKRFDPLLEKITEQYPDVAVIIKPLPFKGESSAKASQAVLSVWKEDPKAFLALHQRLMQKKTMLDNASIEDAMKSTNTSKIKLTDDSLKTLQNNLELSRKLGIQGTPATVIGDTILPGAVDYDQLEIIVKEQLAKVKK
experiment.sample.molecule[0].organismProteus mirabilis
experiment.sample.molecule[0].uniprot_codeA0A1Z1SYD5
experiment.sample.molecule[0].uniprot_range_first64
experiment.sample.molecule[0].uniprot_range_last243
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMKKTLSVLVISSLLFGANVQAAALNAAQEKEVRALVRDTLVSNPEILEEAIMALQTKKAD EQQAQFRQALASEHDALYNDAASPRIGAKDAKLVLVSFTDYNCPYCKRFDPLLEKITEQY PDVAVIIKPLPFKGESSAKASQAVLSVWKEDPKAFLALHQRLMQKKTMLDNASIEDAMKS TNTSKIKLTDDSLKTLQNNLELSRKLGIQGTPATVIGDTILPGAVDYDQLEIIVKEQLAK VKK
experiment.sample.molecule[0].mw20.15
experiment.sample.molecule[0].total_mw20.15
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name25 mM HEPES 150mM NaCl 1mM DTT
experiment.sample.buffer.concentration_unitmM
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive150mM NaCl, 1mM DTT
experiment.sample.buffer.concentration25.0
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameSuppressor of Copper Sensitivity C protein (ScsC) from Proteus mirabilis
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameANSTO
experiment.contributor[0].affiliation[0].addressKirrawee DC, NSW 2232, Australia
experiment.contributor[0].affiliation[0].full_nameAustralian Nuclear Science and Technology Organisation
experiment.contributor[0].affiliation[0].webpage
experiment.contributor[0].contributor_nameAndrew
experiment.contributor[0].contributor_surnameWhitten
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2012-02-29
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time2.0
experiment.number_of_frames14
experiment.wavelengthnull
experiment.sample_detector_distance1.6
experiment.concentration_min2.15
experiment.concentration_max8.85
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.086
experiment.s_max2.487
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[1].p_value0.020808
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pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionX-ray synchrotron radiation scattering data from solutions of Suppressor of Copper Sensitivity C protein (ScsC) from Proteus mirabilis in 25 mM HEPES 150mM NaCl, 1mM DTT, pH 7.5 were collected on the SAXS/WAXS beam line of the Australian Synchrotron (Melbourne, Australia) using a 2D Photon counting Pilatus 1M-W pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). Fourteen successive 2 second frames were collected across solute concentrations of 2.15-8.85 mg/ml. The SAXS data displayed this entry was derived from a 2.15 mg/ml sample. The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering of the solvent-blank was subtracted. The models and corresponding fits include those derived from rigid-body modelling using CORAL (top) and a representative ensemble of six trimeric ScsC structures determined using ensemble optimization method (EOM). The Rg and Dmax distributions derived from EOM are included in the full entry zip archive.
tags[]
intensity_unitnull
experimental_mw69.9
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i00.1006
pddf_i0_error0.00016
guinier_i00.101
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guinier_rg3.65
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porod_volume92.0
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guinier_point_first3
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i0_calibration_standard_datanull
intensities_log_log_plotSASDB94_datloglog_img.png
symmetrynull
last_modified2020-08-28T15:27:57.490687+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDB94
statussuccess
started_at2026-08-11T14:16:30.520236+00:00
finished_at2026-08-11T14:16:42.170445+00:00
source_last_modified2020-08-28T15:27:57.490687+02:00
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查看完整 summary.json 原文
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    "title": "A shape-shifting redox foldase contributes to Proteus mirabilis copper resistance.",
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      "title": "A shape-shifting redox foldase contributes to Proteus mirabilis copper resistance.",
      "author_list": "Furlong EJ, Lo AW, Kurth F, Premkumar L, Totsika M, Achard MES, Halili MA, Heras B, Whitten AE, Choudhury HG, Schembri MA, Martin JL",
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      "doi": "10.1038/ncomms16065",
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查看完整 manifest.json 原文
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