查看完整 summary.json 原文
{
"code": "SASDBB9",
"status": "Published",
"type_of_curve": "Other",
"angular_unit": "1/A",
"project": {
"title": "Structural and biochemical analysis of <i>Escherichia coli</i> ObgE, a central regulator of bacterial persistence.",
"publication": {
"title": "Structural and biochemical analysis of <i>Escherichia coli</i> ObgE, a central regulator of bacterial persistence.",
"author_list": "Gkekas S, Singh RK, Shkumatov AV, Messens J, Fauvart M, Verstraeten N, Michiels J, Versées W",
"journal": "J Biol Chem",
"doi": "10.1074/jbc.M116.761809",
"pmid": "28223358",
"published_date": "2017 Apr 7"
},
"status": "released",
"submitted_date": "2016-09-28",
"released_date": "2017-02-23"
},
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},
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"city": "Saint-Aubin",
"country": "France",
"beamline_name": "SWING",
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"type_of_source": "X-ray synchrotron",
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{
"long_name": "GTPase ObgE/CgtA",
"short_name": "ObgE_FL",
"sequence": "MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGDVWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTGETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLADVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLIEGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELELYSQDLATKPRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIENPVAQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKRSAWSHPQFEK",
"organism": "Escherichia coli",
"uniprot_code": "P42641",
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"oligomerization": "monomer",
"molecular_type": "protein",
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"molecule_source": "biological",
"molecule_description": null
}
],
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"ph": 7.5,
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"name": "GDP bound form of full length ObgE from E.coli (ObgE_FL with GDP)",
"ext_coefficient": null,
"contrast": null,
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},
"contributor": [
{
"affiliation": [
{
"short_name": "VUB",
"address": "Pleinlaan 2 1050 Brussel ",
"full_name": "Vrije Universiteit Brussel",
"webpage": "http://www.vub.ac.be/"
}
],
"contributor_name": "Alexander",
"contributor_surname": "Shkumatov",
"orcid": null
}
],
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"date": "2015-06-17",
"storage_temperature": 10.0,
"cell_temperature": 10.0,
"exposure_time": 2.8,
"number_of_frames": 255,
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},
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"experiment_description": "Synchrotron SAXS data from solutions of the GDP bound form of full length ObgE from E.coli (ObgE_FL with GDP) in 20 mM HEPES, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 µM GDP, pH 7.5, were collected at the SWING beam line at the SOLEIL Synchrotron (Saint-Aubin, France) using a CCD AVIEX detector at a sample-detector distance of 2.0 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Data collection was performed at at 10°C. 255 successive 2.8 second frames were collected through an HPLC-SEC elution peak. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration, then averaged to produce the final SAXS profile displayed in this entry.\r\nThe sample protein includes a C-terminal Strep-tag; frames corresponding to the peak in HPLC-SAXS run were averaged using DATAVER v0.2 (r3709).",
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"intensities_log_log_plot": "SASDBB9_datloglog_img.png",
"symmetry": null,
"last_modified": "2019-12-06T13:07:15.624532+01:00",
"bragg_peak": []
}查看完整 manifest.json 原文
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