SASDBC7

Human NEI like DNA glycosylase 1 (NEIL1)

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2019-12-06T13:07:15.624532+01:00

Experimental MW reported here is calculated from Gnom based I(0) estimates and will be used for publication purpose in future. Guinier I(0) estimates are prone to presence of aggregates/radiation damage and that is why Gnom based I(0) was preferred to calculate the experimental MW.

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Human NEI like DNA glycosylase 1 (NEIL1)

Concentration0.5 – 2.0 Buffer / pH25mM HEPES 300mM NaCl 1mM DTT 10% Glycerol / 7.5
Experimental temperature10.0 Facility / beamlineAdvanced Photon Source (APS), Argonne National Laboratory / BioCAT 18ID
Wavelength0.1033 nmExposure1.0 s × 14

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Endonuclease 8-like 1
View sequence
MPEGPELHLASQFVNEACRALVFGGCVEKSSVSRNPEVPFESSAYRISASARGKELRLILSPLPGAQPQQEPLALVFRFGMSGSFQLVPREELPRHAHLRFYTAPPGPRLALCFVDIRRFGRWDLGGKWQPGRGPCVLQEYQQFRESVLRNLADKAFDRPICEALLDQRFFNGIGNYLRAEILYRLKIPPFEKARSVLEALQQHRPSPELTLSQKIRTKLQNPDLLELCHSVPKEVVQLGGKGYGSESGEEDFAAFRAWLRCYGMPGMSSLQDRHGRTIWFQGDPGPLAPKGRKSRKKKSKATQLSPEDRVEDALPPSKAPSRTRRAKRDLPKRTATQRPEGTSLQQDPEAPTVPKKGRRKGRQAASGHCRPRKVKADIPSLEPEGTSASLEHHHHHH
proteinHomo sapiensQ96FI4—–—monomerMolecule count 144.722 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11089[3]0.00497719–0.328583 1/AHas uncertainty columnMissing 024sasbdb/entries/c7/sasdbc7/source/SASDBC7.dat
▼

2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)15.00.25Å
i0Guinier4.310.03—
i0P(r)4.4110.0441—
mwExperimental48.1—kDa
mwGuinier I(0)50.560.35kDa
mwPorod47.8—kDa
porod_volumePorod81.37—ų
rgGuinier3.550.331Å
rgP(r)3.830.064Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name25mM HEPES 300mM NaCl 1mM DTT 10% GlycerolBuffer concentration—
pH7.5Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2015-03-13Storage / measurement temperature4.0 / 10.0
Exposure time1.0Frames14
Wavelength0.1033Sample-to-detector distance—
SourceX-ray synchrotronDetectorMAR 165 CCD
Facility / beamlineAdvanced Photon Source (APS), Argonne National Laboratory / BioCAT 18ID · Lemont, IL, USA
q range0.05 – 3.286Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:156 field values
Field pathRaw value
codeSASDBC7
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleDestabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase.
project.publication.titleDestabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase.
project.publication.author_listPrakash A, Moharana K, Wallace SS, Doublié S
project.publication.journalNucleic Acids Res
project.publication.doi10.1093/nar/gkw1282
project.publication.pmid27994037
project.publication.published_date2017 Mar 17
project.statusreleased
project.submitted_date2016-11-04
project.released_date2017-01-03
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDBC7.out
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experiment.instrument.detector.typenull
experiment.instrument.detector.nameMAR 165 CCD
experiment.instrument.detector.resolutionnull
experiment.instrument.nameAdvanced Photon Source (APS), Argonne National Laboratory
experiment.instrument.cityLemont, IL
experiment.instrument.countryUSA
experiment.instrument.beamline_nameBioCAT 18ID
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameEndonuclease 8-like 1
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceMPEGPELHLASQFVNEACRALVFGGCVEKSSVSRNPEVPFESSAYRISASARGKELRLILSPLPGAQPQQEPLALVFRFGMSGSFQLVPREELPRHAHLRFYTAPPGPRLALCFVDIRRFGRWDLGGKWQPGRGPCVLQEYQQFRESVLRNLADKAFDRPICEALLDQRFFNGIGNYLRAEILYRLKIPPFEKARSVLEALQQHRPSPELTLSQKIRTKLQNPDLLELCHSVPKEVVQLGGKGYGSESGEEDFAAFRAWLRCYGMPGMSSLQDRHGRTIWFQGDPGPLAPKGRKSRKKKSKATQLSPEDRVEDALPPSKAPSRTRRAKRDLPKRTATQRPEGTSLQQDPEAPTVPKKGRRKGRQAASGHCRPRKVKADIPSLEPEGTSASLEHHHHHH
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeQ96FI4
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMPEGPELHLASQFVNEACRALVFGGCVEKSSVSRNPEVPFESSAYRISASARGKELRLIL SPLPGAQPQQEPLALVFRFGMSGSFQLVPREELPRHAHLRFYTAPPGPRLALCFVDIRRF GRWDLGGKWQPGRGPCVLQEYQQFRENVLRNLADKAFDRPICEALLDQRFFNGIGNYLRA EILYRLKIPPFEKARSVLEALQQHRPSPELTLSQKIRTKLQNPDLLELCHSVPKEVVQLG GKGYGSESGEEDFAAFRAWLRCYGMPGMSSLQDRHGRTIWFQGDPGPLAPKGRKSRKKKS KATQLSPEDRVEDALPPSKAPSRTRRAKRDLPKRTATQRPEGTSLQQDPEAPTVPKKGRR KGRQAASGHCRPRKVKADIPSLEPEGTSAS
experiment.sample.molecule[0].mw44.722
experiment.sample.molecule[0].total_mw44.722
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name25mM HEPES 300mM NaCl 1mM DTT 10% Glycerol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameHuman NEI like DNA glycosylase 1 (NEIL1)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressBurlington, VT, USA
experiment.contributor[0].affiliation[0].full_nameUniversity of Vermont
experiment.contributor[0].affiliation[0].webpagehttps://www.uvm.edu/
experiment.contributor[0].contributor_nameKedar
experiment.contributor[0].contributor_surnameMoharana
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2015-03-13
experiment.storage_temperature4.0
experiment.cell_temperature10.0
experiment.exposure_time1.0
experiment.number_of_frames14
experiment.wavelength0.1033
experiment.sample_detector_distancenull
experiment.concentration_min0.5
experiment.concentration_max2.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.05
experiment.s_max3.286
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].softwareGASBOR
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fits[0].models[0].comment
fits[0].models[0].user177
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fits[0].softwarenull
fits[0].chi_square_value1.07
fits[0].p_value0.120471
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fits[0].fit_lognull
fits[0].software_versionnull
fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionATSAS GNOM 5.0
i0_calibration_standardnull
descriptionExperimental MW reported here is calculated from Gnom based I(0) estimates and will be used for publication purpose in future. Guinier I(0) estimates are prone to presence of aggregates/radiation damage and that is why Gnom based I(0) was preferred to calculate the experimental MW.
experiment_descriptionnull
tags[]
intensity_unitnull
experimental_mw48.1
experimental_mw_errornull
guinier_i0_mw50.56
guinier_i0_mw_error0.35
porod_mw47.8
porod_mw_errornull
pddf_i04.411
pddf_i0_error0.0441
guinier_i04.31
guinier_i0_error0.03
pddf_rg3.83
pddf_rg_error0.064
guinier_rg3.55
guinier_rg_error0.331
pddf_dmax15.0
pddf_dmax_error0.25
porod_volume81.37
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first28
guinier_point_last107
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDBC7_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDBC7
statussuccess
started_at2026-08-11T16:26:54.913519+00:00
finished_at2026-08-11T16:27:04.144154+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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View the complete summary.json source
{
  "code": "SASDBC7",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Destabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase.",
    "publication": {
      "title": "Destabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase.",
      "author_list": "Prakash A, Moharana K, Wallace SS, Doublié S",
      "journal": "Nucleic Acids Res",
      "doi": "10.1093/nar/gkw1282",
      "pmid": "27994037",
      "published_date": "2017 Mar 17"
    },
    "status": "released",
    "submitted_date": "2016-11-04",
    "released_date": "2017-01-03"
  },
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  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBC7_pofr_img.png",
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  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDBC7.sascif",
  "experiment": {
    "instrument": {
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        "resolution": null
      },
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      "city": "Lemont, IL",
      "country": "USA",
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      "beam_geometry": null,
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      "point_source": null,
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    "sample": {
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          "long_name": "Endonuclease 8-like 1",
          "short_name": null,
          "sequence": "MPEGPELHLASQFVNEACRALVFGGCVEKSSVSRNPEVPFESSAYRISASARGKELRLILSPLPGAQPQQEPLALVFRFGMSGSFQLVPREELPRHAHLRFYTAPPGPRLALCFVDIRRFGRWDLGGKWQPGRGPCVLQEYQQFRESVLRNLADKAFDRPICEALLDQRFFNGIGNYLRAEILYRLKIPPFEKARSVLEALQQHRPSPELTLSQKIRTKLQNPDLLELCHSVPKEVVQLGGKGYGSESGEEDFAAFRAWLRCYGMPGMSSLQDRHGRTIWFQGDPGPLAPKGRKSRKKKSKATQLSPEDRVEDALPPSKAPSRTRRAKRDLPKRTATQRPEGTSLQQDPEAPTVPKKGRRKGRQAASGHCRPRKVKADIPSLEPEGTSASLEHHHHHH",
          "organism": "Homo sapiens",
          "uniprot_code": "Q96FI4",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MPEGPELHLASQFVNEACRALVFGGCVEKSSVSRNPEVPFESSAYRISASARGKELRLIL\nSPLPGAQPQQEPLALVFRFGMSGSFQLVPREELPRHAHLRFYTAPPGPRLALCFVDIRRF\nGRWDLGGKWQPGRGPCVLQEYQQFRENVLRNLADKAFDRPICEALLDQRFFNGIGNYLRA\nEILYRLKIPPFEKARSVLEALQQHRPSPELTLSQKIRTKLQNPDLLELCHSVPKEVVQLG\nGKGYGSESGEEDFAAFRAWLRCYGMPGMSSLQDRHGRTIWFQGDPGPLAPKGRKSRKKKS\nKATQLSPEDRVEDALPPSKAPSRTRRAKRDLPKRTATQRPEGTSLQQDPEAPTVPKKGRR\nKGRQAASGHCRPRKVKADIPSLEPEGTSAS",
          "mw": 44.722,
          "total_mw": 44.722,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
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        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Human NEI like DNA glycosylase 1 (NEIL1)",
      "ext_coefficient": null,
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      "dry_vol": null,
      "absorbption": null,
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View the complete manifest.json source
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