SASDBD7

Human Proliferating Cell Nuclear Antigen (PCNA)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2019-12-06T13:07:15.624532+01:00

Experimental MW reported here is from SAXSMoW. PCNA was used to calibrate forward intensity for MW estimation of other scatterers.

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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Human Proliferating Cell Nuclear Antigen (PCNA)

浓度1.0 – 6.0 缓冲液 / pH25mM HEPES 100mM NaCl 1mM DTT / 7.5
实验温度10.0 设备 / 束线Advanced Light Source (ALS) / 12.3.1 (SIBYLS)
波长0.1127 nm曝光0.2 s × 24

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Proliferating cell nuclear antigen
查看序列
MHHHHHHMFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFACICRDLSHIGDAVVISCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIEDEEGS
proteinHomo sapiensP12004—–—trimer分子数 329.669 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1920[3]0.016445–0.528544 1/A含误差列缺失 00sasbdb/entries/d7/sasdbd7/source/SASDBD7.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)9.720.18Å
i0Guinier483.21.4—
i0P(r)486.21.008—
mwExperimental85.9—kDa
mwPorod75.414—kDa
porod_volumePorod128.2—ų
rgGuinier3.440.025Å
rgP(r)3.4390.052Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称25mM HEPES 100mM NaCl 1mM DTT缓冲液浓度—
pH7.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2016-01-20储存 / 测量温度4.0 / 10.0
曝光时间0.2帧数24
波长0.1127样品-探测器距离1.4
光源X-ray synchrotron探测器Pilatus3 X 2M
机构 / 束线Advanced Light Source (ALS) / 12.3.1 (SIBYLS) · Berkeley, CA, USA
q 范围0.164 – 5.285样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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pddfsasbdb/entries/d7/sasdbd7/source/SASDBD7.outdownloaded84573e18a329e3e15fd2e1c32e38696aaf9b054bf5414613c9153b572edae0290d84d下载查看原文件源站
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDBD7
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleDestabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase.
project.publication.titleDestabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase.
project.publication.author_listPrakash A, Moharana K, Wallace SS, Doublié S
project.publication.journalNucleic Acids Res
project.publication.doi10.1093/nar/gkw1282
project.publication.pmid27994037
project.publication.published_date2017 Mar 17
project.statusreleased
project.submitted_date2016-11-04
project.released_date2017-01-03
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sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDBD7.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 X 2M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameAdvanced Light Source (ALS)
experiment.instrument.cityBerkeley, CA
experiment.instrument.countryUSA
experiment.instrument.beamline_name12.3.1 (SIBYLS)
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameProliferating cell nuclear antigen
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceMHHHHHHMFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFACICRDLSHIGDAVVISCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIEDEEGS
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP12004
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationtrimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTY RCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMD LDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNI KLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYK IADMGHLKYYLAPKIEDEEGS
experiment.sample.molecule[0].mw29.669
experiment.sample.molecule[0].total_mw89.007
experiment.sample.molecule[0].number_molecules3
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name25mM HEPES 100mM NaCl 1mM DTT
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameHuman Proliferating Cell Nuclear Antigen (PCNA)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressBurlington, VT, USA
experiment.contributor[0].affiliation[0].full_nameUniversity of Vermont
experiment.contributor[0].affiliation[0].webpagehttps://www.uvm.edu/
experiment.contributor[0].contributor_nameKedar
experiment.contributor[0].contributor_surnameMoharana
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2016-01-20
experiment.storage_temperature4.0
experiment.cell_temperature10.0
experiment.exposure_time0.2
experiment.number_of_frames24
experiment.wavelength0.1127
experiment.sample_detector_distance1.4
experiment.concentration_min1.0
experiment.concentration_max6.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.164
experiment.s_max5.285
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].softwareGASBOR
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modeldummy
fits[0].models[0].software_version
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fits[0].models[0].model_mwnull
fits[0].models[0].bead_radius1.9
fits[0].models[0].lognull
fits[0].models[0].symmetry
fits[0].models[0].comment
fits[0].models[0].user177
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fits[0].softwarenull
fits[0].chi_square_value0.75
fits[0].p_value0.000214
fits[0].fit_residual_plotSASDBD7_fit1_fitresiduals_img.png
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fits[0].fit_lognull
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fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionATSAS GNOM 5.0
i0_calibration_standardnull
descriptionExperimental MW reported here is from SAXSMoW. PCNA was used to calibrate forward intensity for MW estimation of other scatterers.
experiment_descriptionnull
tags[]
intensity_unitnull
experimental_mw85.9
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw75.414
porod_mw_errornull
pddf_i0486.2
pddf_i0_error1.008
guinier_i0483.2
guinier_i0_error1.4
pddf_rg3.439
pddf_rg_error0.052
guinier_rg3.44
guinier_rg_error0.025
pddf_dmax9.72
pddf_dmax_error0.18
porod_volume128.2
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first2
guinier_point_last39
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDBD7_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDBD7
statussuccess
started_at2026-08-11T16:32:30.058203+00:00
finished_at2026-08-11T16:32:38.918350+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
{
  "code": "SASDBD7",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Destabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase.",
    "publication": {
      "title": "Destabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase.",
      "author_list": "Prakash A, Moharana K, Wallace SS, Doublié S",
      "journal": "Nucleic Acids Res",
      "doi": "10.1093/nar/gkw1282",
      "pmid": "27994037",
      "published_date": "2017 Mar 17"
    },
    "status": "released",
    "submitted_date": "2016-11-04",
    "released_date": "2017-01-03"
  },
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          "short_name": null,
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          "oligomerization": "trimer",
          "molecular_type": "protein",
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}
查看完整 manifest.json 原文
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