SASDBE2

Light state solution structure of untagged Aureochrome1a- A´α-LOV-Jα

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2020-07-03T14:55:51.265847+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Light state solution structure of untagged Aureochrome1a- A´α-LOV-Jα

浓度2.0 – 8.0 mg/ml缓冲液 / pH10 mM Tris-HCl, 300 mM NaCl / 8.0
实验温度4.0 设备 / 束线ESRF / BM29
波长0.93 nm曝光10.0 s × 20

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Aureochrome1a- A´α-LOV-Jα (Light State)
查看序列
GSHMDFSFIKALQTAQQNFVVTDPSLPDNPIVYASQGFLNLTGYSLDQILGRNCRFLQGPETDPKAVERIRKAIEQGNDMSVCLLNYRVDGTTFWNQFFIAALRDAGGNVTNFVGVQCKVSDQYAATVTKQQEEEEEAAANDDED
proteinPhaeodactylum tricornutum——–—dimer分子数 216.1 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11043[3]0.0364283–4.91162 1/nm含误差列缺失 01sasbdb/entries/e2/sasdbe2/source/SASDBE2.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)9.8—nm
i0Guinier48.1——
i0P(r)48.35——
mwExperimental48.1—kDa
mwGuinier I(0)48.1—kDa
mwPorod44.2—kDa
porod_volumePorod66.4—nm³
rgGuinier2.6—nm
rgP(r)2.65—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称10 mM Tris-HCl, 300 mM NaCl缓冲液浓度10.0 mM
pH8.0添加剂300 mM NaCl
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2015-09-09储存 / 测量温度4.0 / 4.0
曝光时间10.0帧数20
波长0.93样品-探测器距离2.43
光源X-ray synchrotron探测器Pilatus 1M
机构 / 束线ESRF / BM29 · Grenoble, France
q 范围0.036 – 4.912样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDBE2
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleStructure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum.
project.publication.titleStructure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum.
project.publication.author_listBanerjee A, Herman E, Kottke T, Essen LO
project.publication.journalStructure
project.publication.doi10.1016/j.str.2015.10.022
project.publication.pmid26688213
project.publication.published_date2016 Jan 5
project.statusreleased
project.submitted_date2015-06-18
project.released_date2016-01-05
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experiment.instrument.countryFrance
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pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionX-ray synchrotron radiation scattering data were collected from solutions of Aureochrome1a-A´α-LOV-Jα in 10 mM Tris 300 mM NaCl (thrombin-cleaved N-terminal affinity tag). SAXS data were measured on the BM29 beam line on the storage ring ESRF (Grenoble, France) using a 2D Photon counting Pilatus 1M-W pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle. s-range 0.036-4.9 nm-1). Different solute concentrations in the range 2.00-8.00 mg/mL were measured. 10 successive 20 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected were merged with the highest concentration high angle data to yield the final composite scattering curve. The analysis was performed using the ATSAS package. The displayed model is an averaged and volume corrected dummy atom representation (DAMFILT). For further sequence information refer to the Joint Genome Institute (JGI) entry JGI-49116.
tags[]
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experimental_mw48.1
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last_modified2020-07-03T14:55:51.265847+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDBE2
statussuccess
started_at2026-08-11T16:39:22.782858+00:00
finished_at2026-08-11T16:39:31.888855+00:00
source_last_modified2020-07-03T14:55:51.265847+02:00
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查看完整 summary.json 原文
{
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  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
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    "title": "Structure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum.",
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      "title": "Structure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum.",
      "author_list": "Banerjee A, Herman E, Kottke T, Essen LO",
      "journal": "Structure",
      "doi": "10.1016/j.str.2015.10.022",
      "pmid": "26688213",
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查看完整 manifest.json 原文
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