SASDBF3

Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains.

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2019-12-06T13:07:15.624532+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains.

浓度— – — 缓冲液 / pH25 Tris mM 100 mM NaCl 1 mM 2-mercaptoethanol / 7.5
实验温度— 设备 / 束线Brazilian Synchrotron Light Laboratory / SAXS2 Beamline
波长— nm曝光— s × —

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains
查看序列
MGSSHHHHHHSSGLVPRGSHMTETFAFQAEINQLMSLIINTFYSNKEIFLRELISNASDACDKIRYQSLTDPSVLGEETHLRVRVVPDKANKTLTVEDNGIGMTKADLVNNLGTIARSGTKAFMEALEAGGDMSMIGQFGVGFYSAYLVADRVTVVSKNNSDEAYVWESSAGGTFTITSVPESDMKRGTRITLHLKEDQQEYLEERRVKELIKKHSEFIGYDIELMVEKTAEKEVTDEDEEEDESKKKSCGDEGEPKVEEVTEGGEDKKKKTKKVKEVTKTYEVQNKHKPLWTRDPKDVTKEEYAAFYKAISNDWEDPAATKHFSVEGQLEFRAIAFVPKRAPFDMFEPNKKRNNIKLYVRRVFIMDNCEDLCPDWLGFVKGVVDSEDLPLNISRENLQQNKILKVIRKNIVKKCLELFEEIAENKEDYKQFYEQFGKNIKLGIHEDTANRKKLMELLRFYSTESGEEMTTLKDYVTRMKPEQKSIYYITGDSKKKLESSPFIEKARRCGLEVLFMTEPIDEYVMQQVKDFEDKKFACLTKE
proteinLeishmania braziliensis——–—monomer分子数 162.0 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1227[2]0.1906827–3.297047 1/nm无误差列缺失 2270sasbdb/entries/f3/sasdbf3/source/SASDBF3.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)13.0—nm
i0Guinier0.0013——
i0P(r)0.1311——
mwExperimental67.0—kDa
porod_volumePorod89.0—nm³
rgGuinier3.15—nm
rgP(r)3.24—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称25 Tris mM 100 mM NaCl 1 mM 2-mercaptoethanol缓冲液浓度25.0 mM
pH7.5添加剂100 mM NaCl, 1 mM 2-mercaptoethanol
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2011-09-01储存 / 测量温度— / —
曝光时间—帧数—
波长—样品-探测器距离—
光源X-ray synchrotron探测器MAR 165 CCD
机构 / 束线Brazilian Synchrotron Light Laboratory / SAXS2 Beamline · Campinas, Brazil
q 范围0.191 – 3.297样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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pddfsasbdb/entries/f3/sasdbf3/source/SASDBF3.outdownloaded11488212c12269680ff4f04f7b3bda02c45c043d30502383e9823abaf643e0788828dc下载查看原文件源站
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(6 项)
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pddf:来源记录
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sascif:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:184 个字段值
字段路径原始值
codeSASDBF3
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleInsights on the structural dynamics of Leishmania braziliensis Hsp90 molecular chaperone by small angle X-ray scattering.
project.publication.titleInsights on the structural dynamics of Leishmania braziliensis Hsp90 molecular chaperone by small angle X-ray scattering.
project.publication.author_listSeraphim TV, Silva KP, Dores-Silva PR, Barbosa LR, Borges JC
project.publication.journalInt J Biol Macromol
project.publication.doi10.1016/j.ijbiomac.2017.01.058
project.publication.pmid28104372
project.publication.published_date2017 Apr
project.statusreleased
project.submitted_date2016-01-18
project.released_date2016-01-21
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experiment.instrument.detector.resolutionnull
experiment.instrument.nameBrazilian Synchrotron Light Laboratory
experiment.instrument.cityCampinas
experiment.instrument.countryBrazil
experiment.instrument.beamline_nameSAXS2 Beamline
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameLeishmania braziliensis heat shock protein 90 (Hsp90) N and M domains
experiment.sample.molecule[0].short_nameL. brazilien.Hsp90NM
experiment.sample.molecule[0].sequenceMGSSHHHHHHSSGLVPRGSHMTETFAFQAEINQLMSLIINTFYSNKEIFLRELISNASDACDKIRYQSLT DPSVLGEETHLRVRVVPDKANKTLTVEDNGIGMTKADLVNNLGTIARSGTKAFMEALEAGGDMSMIGQFG VGFYSAYLVADRVTVVSKNNSDEAYVWESSAGGTFTITSVPESDMKRGTRITLHLKEDQQEYLEERRVKE LIKKHSEFIGYDIELMVEKTAEKEVTDEDEEEDESKKKSCGDEGEPKVEEVTEGGEDKKKKTKKVKEVTK TYEVQNKHKPLWTRDPKDVTKEEYAAFYKAISNDWEDPAATKHFSVEGQLEFRAIAFVPKRAPFDMFEPN KKRNNIKLYVRRVFIMDNCEDLCPDWLGFVKGVVDSEDLPLNISRENLQQNKILKVIRKNIVKKCLELFE EIAENKEDYKQFYEQFGKNIKLGIHEDTANRKKLMELLRFYSTESGEEMTTLKDYVTRMKPEQKSIYYIT GDSKKKLESSPFIEKARRCGLEVLFMTEPIDEYVMQQVKDFEDKKFACLTKE
experiment.sample.molecule[0].organismLeishmania braziliensis
experiment.sample.molecule[0].uniprot_codenull
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experiment.sample.molecule[0].uniprot_range_lastnull
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experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequencenull
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experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
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experiment.sample.buffer.commentnull
experiment.sample.buffer.additive100 mM NaCl, 1 mM 2-mercaptoethanol
experiment.sample.buffer.concentration25.0
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameLeishmania braziliensis heat shock protein 90 (Hsp90) N and M domains.
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
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experiment.sample.mixturenull
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experiment.contributor[0].affiliation[0].full_nameInstituto de Física, Universidade de São Paulo
experiment.contributor[0].affiliation[0].webpage
experiment.contributor[0].contributor_nameLeandro
experiment.contributor[0].contributor_surnameBarbosa
experiment.contributor[0].orcidnull
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experiment.contributor[1].affiliation[0].webpage
experiment.contributor[1].contributor_nameJúlio
experiment.contributor[1].contributor_surnameBorges
experiment.contributor[1].orcidnull
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experiment.contributor[2].affiliation[0].webpage
experiment.contributor[2].contributor_namePaulo
experiment.contributor[2].contributor_surnameDores-Silva
experiment.contributor[2].orcidnull
experiment.contributor[3].affiliation[0].short_namenull
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experiment.contributor[3].affiliation[0].full_nameInstituto de Química de São Carlos
experiment.contributor[3].affiliation[0].webpage
experiment.contributor[3].contributor_nameThiago
experiment.contributor[3].contributor_surnameSeraphim
experiment.contributor[3].orcidnull
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experiment.contributor[4].affiliation[0].addressSão Carlos - SP - Brasil
experiment.contributor[4].affiliation[0].full_nameInstituto de Química de São Carlos
experiment.contributor[4].affiliation[0].webpage
experiment.contributor[4].contributor_nameKelly
experiment.contributor[4].contributor_surnameSilva
experiment.contributor[4].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2011-09-01
experiment.storage_temperaturenull
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experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].p_value0.001616
fits[0].fit_residual_plotSASDBF3_fit1_fitresiduals_img.png
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fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionX-ray synchrotron radiation scattering data from solutions of Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains in 25 mM Tris 100 mM NaCl, 1 mM 2-mercaptoethanol were collected on the SAXS2 Beamline camera on the storage ring Brazilian Synchrotron Light Laboratory (Campinas, Brazil) using a MAR Image Plate detector (s = 4π sin θ/λ, where 2θ is the scattering angle). The data were normalized to the intensity of the transmitted beam and radially averaged. The scattering of the solvent-blank was subtracted. The low angle data collected were obtained from a single concentration scattering curve.
tags[]
intensity_unitnull
experimental_mw67.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
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pddf_rg_errornull
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porod_volume_errornull
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guinier_point_last17
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDBF3_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDBF3
statussuccess
started_at2026-08-11T13:58:53.457443+00:00
finished_at2026-08-11T13:59:03.193350+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
{
  "code": "SASDBF3",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Insights on the structural dynamics of Leishmania braziliensis Hsp90 molecular chaperone by small angle X-ray scattering.",
    "publication": {
      "title": "Insights on the structural dynamics of Leishmania braziliensis Hsp90 molecular chaperone by small angle X-ray scattering.",
      "author_list": "Seraphim TV, Silva KP, Dores-Silva PR, Barbosa LR, Borges JC",
      "journal": "Int J Biol Macromol",
      "doi": "10.1016/j.ijbiomac.2017.01.058",
      "pmid": "28104372",
      "published_date": "2017 Apr"
    },
    "status": "released",
    "submitted_date": "2016-01-18",
    "released_date": "2016-01-21"
  },
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查看完整 manifest.json 原文
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