SASDBF6

Deglycosylated myelin-associated glycoprotein full extracellular domain (immunoglobulin domains 1-5)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2019-12-06T13:07:15.624532+01:00

Endo-Hf-deglycosylated full extracellular domain of Myelin-associated glycoprotein (Ig1-5).

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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Deglycosylated myelin-associated glycoprotein full extracellular domain (immunoglobulin domains 1-5)

浓度— – 2.13 缓冲液 / pH20 mM HEPES 150 mM NaCl / 7.5
实验温度20.0 设备 / 束线ESRF / BM29
波长0.0992 nm曝光2.0 s × 10

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Myelin-associated glycoprotein Ig domains 1-5
查看序列
GHWGAWMPSTISAFEGTCVSIPCRFDFPDELRPAVVHGVWYFNSPYPKNYPPVVFKSRTQVVHESFQGRSRLLGDLGLRNCTLLLSTLSPELGGKYYFRGDLGGYNQYTFSEHSVLDIVNTPNIVVPPEVVAGTEVEVSCMVPDNCPELRPELSWLGHEGLGEPTVLGRLREDEGTWVQVSLLHFVPTREANGHRLGCQAAFPNTTLQFEGYASLDVKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGMVLREAVAKSLYLDLEEVTPGEDGVYACLAENAYGQDNRTVELSVMYAPWKPTVNGTVVAVEGETVSILCSTQSNPDPILTIFKEKQILATVIYESQLQLELPAVTPEDDGEYWCVAENQYGQRATAFNLSVEFAPIILLESHCAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNETEREFVYSERSGLLLTSILTIRGQAQAPPRVICTSRNLYGTQSLELPFQGAHR
proteinMus musculusP2091720–508dimer分子数 253.963 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11043[3]0.0363402–4.969332 1/nm含误差列缺失 05sasbdb/entries/f6/sasdbf6/source/SASDBF6.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)25.5—nm
i0Guinier75.370.27—
i0P(r)74.90.1—
mwExperimental56.8—kDa
mwGuinier I(0)75.4—kDa
mwPorod97.0—kDa
porod_volumePorod166.2—nm³
rgGuinier7.280.05nm
rgP(r)7.430.01nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM HEPES 150 mM NaCl缓冲液浓度20.0 mM
pH7.5添加剂150 mM NaCl
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2014-09-11储存 / 测量温度20.0 / 20.0
曝光时间2.0帧数10
波长0.0992样品-探测器距离2.87
光源X-ray synchrotron探测器Pilatus 1M
机构 / 束线ESRF / BM29 · Grenoble, France
q 范围0.036 – 4.969样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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full_entry_zipsasbdb/entries/f6/sasdbf6/source/SASDBF6.zipdownloaded38078610810959ddd868f66f03a1679455435c7c71cce782053f5b92fa4c8af7fc632b下载查看原文件源站
pddfsasbdb/entries/f6/sasdbf6/source/SASDBF6.outdownloaded23593e79fddc350d712fed62f3e014fb07cbb0bf0387795b377d94edbb041e177c7bb下载查看原文件源站
sascifsasbdb/entries/f6/sasdbf6/source/SASDBF6.sascifdownloaded870565f4795a90406a5108654ccc4b0a282f706b1c65f7ed31df449815d2b3f25ac28d下载查看原文件源站
summarysasbdb/entries/f6/sasdbf6/source/summary.jsondownloaded83732fea9276b51ee8e24f3a4d3ecb58211a8f3846fbd1207ff35cdc7ee8f628e597下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:159 个字段值
字段路径原始值
codeSASDBF6
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleStructural basis of myelin-associated glycoprotein adhesion and signalling.
project.publication.titleStructural basis of myelin-associated glycoprotein adhesion and signalling.
project.publication.author_listPronker MF, Lemstra S, Snijder J, Heck AJ, Thies-Weesie DM, Pasterkamp RJ, Janssen BJ
project.publication.journalNat Commun
project.publication.doi10.1038/ncomms13584
project.publication.pmid27922006
project.publication.published_date2016 Dec 6
project.statusreleased
project.submitted_date2016-07-24
project.released_date2016-12-13
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDBF6.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDBF6.dat
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pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBF6_pofr_img.png
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sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDBF6.sascif
experiment.instrument.detector.typeDectris
experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameMyelin-associated glycoprotein Ig domains 1-5
experiment.sample.molecule[0].short_nameMAG
experiment.sample.molecule[0].sequenceGHWGAWMPSTI SAFEGTCVSI PCRFDFPDEL RPAVVHGVWY FNSPYPKNYP PVVFKSRTQV VHESFQGRSR LLGDLGLRNC TLLLSTLSPE LGGKYYFRGD LGGYNQYTFS EHSVLDIVNT PNIVVPPEVV AGTEVEVSCM VPDNCPELRP ELSWLGHEGL GEPTVLGRLR EDEGTWVQVS LLHFVPTREA NGHRLGCQAA FPNTTLQFEG YASLDVKYPP VIVEMNSSVE AIEGSHVSLL CGADSNPPPL LTWMRDGMVL REAVAKSLYL DLEEVTPGED GVYACLAENA YGQDNRTVEL SVMYAPWKPT VNGTVVAVEG ETVSILCSTQ SNPDPILTIF KEKQILATVI YESQLQLELP AVTPEDDGEY WCVAENQYGQ RATAFNLSVE FAPIILLESH CAAARDTVQC LCVVKSNPEP SVAFELPSRN VTVNETEREF VYSERSGLLL TSILTIRGQA QAPPRVICTS RNLYGTQSLE LPFQGAHR
experiment.sample.molecule[0].organismMus musculus
experiment.sample.molecule[0].uniprot_codeP20917
experiment.sample.molecule[0].uniprot_range_first20
experiment.sample.molecule[0].uniprot_range_last508
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMIFLATLPLFWIMISASRGGHWGAWMPSTISAFEGTCVSIPCRFDFPDELRPAVVHGVWY FNSPYPKNYPPVVFKSRTQVVHESFQGRSRLLGDLGLRNCTLLLSTLSPELGGKYYFRGD LGGYNQYTFSEHSVLDIVNTPNIVVPPEVVAGTEVEVSCMVPDNCPELRPELSWLGHEGL GEPTVLGRLREDEGTWVQVSLLHFVPTREANGHRLGCQAAFPNTTLQFEGYASLDVKYPP VIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGMVLREAVAKSLYLDLEEVTPGED GVYACLAENAYGQDNRTVELSVMYAPWKPTVNGTVVAVEGETVSILCSTQSNPDPILTIF KEKQILATVIYESQLQLELPAVTPEDDGEYWCVAENQYGQRATAFNLSVEFAPIILLESH CAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNETEREFVYSERSGLLLTSILTIRGQA QAPPRVICTSRNLYGTQSLELPFQGAHRLMWAKIGPVGAVVAFAILIAIVCYITQTRRKK NVTESSSFSGGDNPHVLYSPEFRISGAPDKYESEKQRLGSERRLLGLRGESPELDLSYSH SDLGKRPTKDSYTLTEELAEYAEIRVK
experiment.sample.molecule[0].mw53.963
experiment.sample.molecule[0].total_mw107.927
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM HEPES 150 mM NaCl
experiment.sample.buffer.concentration_unitmM
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive150 mM NaCl
experiment.sample.buffer.concentration20.0
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameDeglycosylated myelin-associated glycoprotein full extracellular domain (immunoglobulin domains 1-5)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressUtrecht, Netherlands
experiment.contributor[0].affiliation[0].full_nameUtrecht University
experiment.contributor[0].affiliation[0].webpagehttp://www.uu.nl/
experiment.contributor[0].contributor_nameMatti
experiment.contributor[0].contributor_surnamePronker
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2014-09-11
experiment.storage_temperature20.0
experiment.cell_temperature20.0
experiment.exposure_time2.0
experiment.number_of_frames10
experiment.wavelength0.0992
experiment.sample_detector_distance2.87
experiment.concentration_minnull
experiment.concentration_max2.13
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.036
experiment.s_max4.969
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/MAG5D_refine_95-cootdimer_img.png
fits[0].models[0].softwareOligomer
fits[0].models[0].pdb_link[0].pdb_code5lf5
fits[0].models[0].pdb_link[0].ext_code5LF5
fits[0].models[0].pdb_link[0].ext_typepdb
fits[0].models[0].pdb_link[0].difference_with_modelderived
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modelatomic
fits[0].models[0].software_version
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fits[0].models[0].model_mw110.5
fits[0].models[0].bead_radius1.9
fits[0].models[0].lognull
fits[0].models[0].symmetry
fits[0].models[0].comment
fits[0].models[0].user52
fits[0].fit_unit1/A
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fits[0].softwarenull
fits[0].chi_square_value3.72
fits[0].p_value0.0
fits[0].fit_residual_plotSASDBF6_fit1_fitresiduals_img.png
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fits[0].fit_lognull
fits[0].software_versionnull
fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionGnom 4.5a
i0_calibration_standardnull
descriptionEndo-Hf-deglycosylated full extracellular domain of Myelin-associated glycoprotein (Ig1-5).
experiment_descriptionSynchrotron SAXS data from solutions of deglycosylated myelin-associated glycoprotein (Ig domains 1-5) in HEPES, pH 7.5 were collected on the BM29 camera on the storage ring ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). One solute concentration of 2.13 mg/ml was measured at 20°C. 10 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the curve was scaled for protein concentration.
tags[]
intensity_unitnull
experimental_mw56.8
experimental_mw_errornull
guinier_i0_mw75.4
guinier_i0_mw_errornull
porod_mw97.0
porod_mw_errornull
pddf_i074.9
pddf_i0_error0.1
guinier_i075.37
guinier_i0_error0.27
pddf_rg7.43
pddf_rg_error0.01
guinier_rg7.28
guinier_rg_error0.05
pddf_dmax25.5
pddf_dmax_errornull
porod_volume166.2
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first9
guinier_point_last24
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDBF6_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDBF6
statussuccess
started_at2026-08-11T16:43:25.549179+00:00
finished_at2026-08-11T16:43:35.150195+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
{
  "code": "SASDBF6",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Structural basis of myelin-associated glycoprotein adhesion and signalling.",
    "publication": {
      "title": "Structural basis of myelin-associated glycoprotein adhesion and signalling.",
      "author_list": "Pronker MF, Lemstra S, Snijder J, Heck AJ, Thies-Weesie DM, Pasterkamp RJ, Janssen BJ",
      "journal": "Nat Commun",
      "doi": "10.1038/ncomms13584",
      "pmid": "27922006",
      "published_date": "2016 Dec 6"
    },
    "status": "released",
    "submitted_date": "2016-07-24",
    "released_date": "2016-12-13"
  },
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        "resolution": 172.0
      },
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      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
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          "long_name": "Myelin-associated glycoprotein Ig domains 1-5",
          "short_name": "MAG",
          "sequence": "GHWGAWMPSTI SAFEGTCVSI PCRFDFPDEL\r\nRPAVVHGVWY FNSPYPKNYP PVVFKSRTQV VHESFQGRSR LLGDLGLRNC\r\nTLLLSTLSPE LGGKYYFRGD LGGYNQYTFS EHSVLDIVNT PNIVVPPEVV\r\nAGTEVEVSCM VPDNCPELRP ELSWLGHEGL GEPTVLGRLR EDEGTWVQVS\r\nLLHFVPTREA NGHRLGCQAA FPNTTLQFEG YASLDVKYPP VIVEMNSSVE\r\nAIEGSHVSLL CGADSNPPPL LTWMRDGMVL REAVAKSLYL DLEEVTPGED\r\nGVYACLAENA YGQDNRTVEL SVMYAPWKPT VNGTVVAVEG ETVSILCSTQ\r\nSNPDPILTIF KEKQILATVI YESQLQLELP AVTPEDDGEY WCVAENQYGQ\r\nRATAFNLSVE FAPIILLESH CAAARDTVQC LCVVKSNPEP SVAFELPSRN\r\nVTVNETEREF VYSERSGLLL TSILTIRGQA QAPPRVICTS RNLYGTQSLE\r\nLPFQGAHR",
          "organism": "Mus musculus",
          "uniprot_code": "P20917",
          "uniprot_range_first": 20,
          "uniprot_range_last": 508,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MIFLATLPLFWIMISASRGGHWGAWMPSTISAFEGTCVSIPCRFDFPDELRPAVVHGVWY\nFNSPYPKNYPPVVFKSRTQVVHESFQGRSRLLGDLGLRNCTLLLSTLSPELGGKYYFRGD\nLGGYNQYTFSEHSVLDIVNTPNIVVPPEVVAGTEVEVSCMVPDNCPELRPELSWLGHEGL\nGEPTVLGRLREDEGTWVQVSLLHFVPTREANGHRLGCQAAFPNTTLQFEGYASLDVKYPP\nVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGMVLREAVAKSLYLDLEEVTPGED\nGVYACLAENAYGQDNRTVELSVMYAPWKPTVNGTVVAVEGETVSILCSTQSNPDPILTIF\nKEKQILATVIYESQLQLELPAVTPEDDGEYWCVAENQYGQRATAFNLSVEFAPIILLESH\nCAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNETEREFVYSERSGLLLTSILTIRGQA\nQAPPRVICTSRNLYGTQSLELPFQGAHRLMWAKIGPVGAVVAFAILIAIVCYITQTRRKK\nNVTESSSFSGGDNPHVLYSPEFRISGAPDKYESEKQRLGSERRLLGLRGESPELDLSYSH\nSDLGKRPTKDSYTLTEELAEYAEIRVK",
          "mw": 53.963,
          "total_mw": 107.927,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "20 mM HEPES 150 mM NaCl",
        "concentration_unit": "mM",
        "comment": null,
        "additive": "150 mM NaCl",
        "concentration": 20.0,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Deglycosylated myelin-associated glycoprotein full extracellular domain (immunoglobulin domains 1-5)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Utrecht, Netherlands",
            "full_name": "Utrecht University",
            "webpage": "http://www.uu.nl/"
          }
        ],
        "contributor_name": "Matti",
        "contributor_surname": "Pronker",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2014-09-11",
    "storage_temperature": 20.0,
    "cell_temperature": 20.0,
    "exposure_time": 2.0,
    "number_of_frames": 10,
    "wavelength": 0.0992,
    "sample_detector_distance": 2.87,
    "concentration_min": null,
    "concentration_max": 2.13,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.036,
    "s_max": 4.969,
    "total_exposure_time": null,
    "seccolumn": null
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  "fits": [
    {
      "models": [
        {
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          "software": "Oligomer",
          "pdb_link": [
            {
              "pdb_code": "5lf5",
              "ext_code": "5LF5",
              "ext_type": "pdb",
              "difference_with_model": "derived"
            }
          ],
          "model_title": null,
          "type_of_model": "atomic",
          "software_version": "",
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDBF6_fit1_model1.pdb",
          "model_mw": 110.5,
          "bead_radius": 1.9,
          "log": null,
          "symmetry": "",
          "comment": "",
          "user": 52
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDBF6_fit1_fit_img.png",
      "software": null,
      "chi_square_value": 3.72,
      "p_value": 0.0,
      "fit_residual_plot": "SASDBF6_fit1_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDBF6_fit1.fit",
      "fit_log": null,
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  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "Gnom 4.5a",
  "i0_calibration_standard": null,
  "description": "Endo-Hf-deglycosylated full extracellular domain of Myelin-associated glycoprotein (Ig1-5).",
  "experiment_description": "Synchrotron SAXS data from solutions of deglycosylated myelin-associated glycoprotein (Ig domains 1-5) in HEPES, pH 7.5 were collected on the BM29 camera on the storage ring ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). One solute concentration of 2.13 mg/ml was measured at 20°C. 10 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the curve was scaled for protein concentration.",
  "tags": [],
  "intensity_unit": null,
  "experimental_mw": 56.8,
  "experimental_mw_error": null,
  "guinier_i0_mw": 75.4,
  "guinier_i0_mw_error": null,
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  "porod_mw_error": null,
  "pddf_i0": 74.9,
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  "pddf_rg": 7.43,
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  "guinier_rg_error": 0.05,
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  "porod_volume": 166.2,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 9,
  "guinier_point_last": 24,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDBF6_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
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