SASDBH2

Mutant archaeal biofilm regulator 1 (ABfR1) phosphomimic Y84E S87D

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2019-12-06T13:07:15.624532+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Mutant archaeal biofilm regulator 1 (ABfR1) phosphomimic Y84E S87D

浓度— – 3.8 缓冲液 / pH20 mM HEPES 200 mM NaCl / 7.5
实验温度4.0 设备 / 束线ESRF / BM29
波长0.93 nm曝光20.0 s × 10

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Archaeal biofilm regulator 1 (AbfR1) mutant Y84E S87D phosphomimic mutant
查看序列
MSIEITEKYVILKRFLSVAYGLSESEIDTFLRILESKEGKDIDAISAELKISKSRTSIILKKLSDAGLVDKEKGDNMKGGRPKEVYDVNKEELKNRLIAKSEELCKELNQVIKSFI
proteinSulfolobus acidocaldariusQ4JBH11–116dimer分子数 213.16 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11043[3]0.0326864–4.9336 1/nm含误差列缺失 01sasbdb/entries/h2/sasdbh2/source/SASDBH2.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)9.5—nm
i0Guinier32.2——
i0P(r)32.3——
mwExperimental32.3—kDa
mwGuinier I(0)32.25—kDa
mwPorod33.3—kDa
porod_volumePorod50.93—nm³
rgGuinier2.64—nm
rgP(r)2.69—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM HEPES 200 mM NaCl缓冲液浓度20.0 mM
pH7.5添加剂200 mM NaCl
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2015-08-27储存 / 测量温度4.0 / 4.0
曝光时间20.0帧数10
波长0.93样品-探测器距离2.43
光源X-ray synchrotron探测器Pilatus 1M
机构 / 束线ESRF / BM29 · Grenoble, France
q 范围0.033 – 4.934样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDBH2
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleWing phosphorylation is a major functional determinant of the Lrs14-type biofilm and motility regulator AbfR1 in Sulfolobus acidocaldarius.
project.publication.titleWing phosphorylation is a major functional determinant of the Lrs14-type biofilm and motility regulator AbfR1 in Sulfolobus acidocaldarius.
project.publication.author_listLi L, Banerjee A, Bischof LF, Maklad HR, Hoffmann L, Henche AL, Veliz F, Bildl W, Schulte U, Orell A, Essen LO, Peeters E, Albers SV
project.publication.journalMol Microbiol
project.publication.doi10.1111/mmi.13735
project.publication.pmid28628237
project.publication.published_date2017 Sep
project.statusreleased
project.submitted_date2015-10-01
project.released_date2017-05-23
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experiment.instrument.countryFrance
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experiment.sample.molecule[0].short_nameABfR1 mutant Y84E S8
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experiment.sample.molecule[0].organismSulfolobus acidocaldarius
experiment.sample.molecule[0].uniprot_codeQ4JBH1
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experiment.sample.buffer.pkanull
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pddf_softwareATSAS GNOM
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i0_calibration_standardnull
descriptionnull
experiment_descriptionX-ray synchrotron radiation scattering data from solutions of N terminal affinity tag cleaved (by thrombin) AbfR1 Y84E S87D phosphomimic mutant in 20mMHEPES pH7.5, 200mM NaCl were collected on the BM29 beam line on the storage ring ESRF (Grenoble, France) using a 2D Photon counting Pilatus 1M pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). A 3.8 mg/mL concentration was measured. 10 successive 20 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The analysis was performed using the ATSAS package. The displayed model is an averaged and volume corrected dummy atom representation (DAMFILT) after imposing P2 symmetry.
tags[]
intensity_unitnull
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intensities_log_log_plotSASDBH2_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDBH2
statussuccess
started_at2026-08-11T13:38:39.876601+00:00
finished_at2026-08-11T13:38:48.378087+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
{
  "code": "SASDBH2",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Wing phosphorylation is a major functional determinant of the Lrs14-type biofilm and motility regulator AbfR1 in Sulfolobus acidocaldarius.",
    "publication": {
      "title": "Wing phosphorylation is a major functional determinant of the Lrs14-type biofilm and motility regulator AbfR1 in Sulfolobus acidocaldarius.",
      "author_list": "Li L, Banerjee A, Bischof LF, Maklad HR, Hoffmann L, Henche AL, Veliz F, Bildl W, Schulte U, Orell A, Essen LO, Peeters E, Albers SV",
      "journal": "Mol Microbiol",
      "doi": "10.1111/mmi.13735",
      "pmid": "28628237",
      "published_date": "2017 Sep"
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}
查看完整 manifest.json 原文
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