Dps1 truncated, DNA binding protein under starvation conditions (SEC-SAXS)

{
  "code": "SASDBH7",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/nm",
  "project": {
    "title": "SAXS Structural Studies of Dps from Deinococcus radiodurans Highlights the Conformation of the Mobile N-Terminal Extensions.",
    "publication": {
      "title": "SAXS Structural Studies of Dps from Deinococcus radiodurans Highlights the Conformation of the Mobile N-Terminal Extensions.",
      "author_list": "Santos SP, Cuypers MG, Round A, Finet S, Narayanan T, Mitchell EP, Romão CV",
      "journal": "J Mol Biol",
      "doi": "10.1016/j.jmb.2017.01.008",
      "pmid": "28088481",
      "published_date": "2017 Mar 10"
    },
    "status": "released",
    "submitted_date": "2016-11-04",
    "released_date": "2017-02-15"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDBH7.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDBH7.dat",
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  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBH7_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBH7_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBH7_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDBH7.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": "Dectris",
        "name": "Pilatus 1M",
        "resolution": 172.0
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "N-terminal truncated DNA protection during starvation protein 1",
          "short_name": "Dps1t",
          "sequence": "HYLEEKEFQTVAETLQRNLATTISLYLKFKKYHWDIRGRFFRDLHLAYDEFIAEIFPSIDEQAERLVALG\r\nGSPLAAPADLARYSTVQVPQETVRDARTQVADLVQDLSRVGKGYRDDSQACDEANDPVTA\r\nDMYNGYAATIDKIRWMLQAIMDDERLD",
          "organism": "Deinococcus radiodurans R1",
          "uniprot_code": "Q9RS64",
          "uniprot_range_first": 51,
          "uniprot_range_last": 207,
          "oligomerization": "dodecamer",
          "molecular_type": "protein",
          "uniprot_sequence": "MTKKSTKSEAASKTKKSGVPETGAQGVRAGGADHADAAHLGTVNNALVNHHYLEEKEFQT\nVAETLQRNLATTISLYLKFKKYHWDIRGRFFRDLHLAYDEFIAEIFPSIDEQAERLVALG\nGSPLAAPADLARYSTVQVPQETVRDARTQVADLVQDLSRVGKGYRDDSQACDEANDPVTA\nDMYNGYAATIDKIRWMLQAIMDDERLD",
          "mw": 18.037,
          "total_mw": 216.444,
          "number_molecules": 12,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "20 mM Tris-HCl, 150 mM NaCl",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Dps1 truncated, DNA binding protein under starvation conditions (SEC-SAXS)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Oeiras, Portugal",
            "full_name": "Instituto de Tecnologia Química e Biológica António Xavier, Universidade de Lisboa",
            "webpage": "http://www.itqb.unl.pt"
          }
        ],
        "contributor_name": "Sandra",
        "contributor_surname": "Santos",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2013-11-22",
    "storage_temperature": 20.0,
    "cell_temperature": 20.0,
    "exposure_time": 1.0,
    "number_of_frames": 1500,
    "wavelength": 0.09919,
    "sample_detector_distance": 2.81,
    "concentration_min": null,
    "concentration_max": 10.0,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.063,
    "s_max": 4.485,
    "total_exposure_time": null,
    "seccolumn": null
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          "bead_radius": 1.9,
          "log": null,
          "symmetry": "P23",
          "comment": "",
          "user": 162
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      ],
      "fit_unit": "1/A",
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  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": null,
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data, I(s) vs s (s = 4π sin θ/λ, where 2θ is the scattering angle) were collected from a sample of truncated Dps1, (DNA binding protein under starvation conditions) using continuous-flow size-exclusion chromatography SAXS (SEC-SAXS) at the BM29 beam line on the ESRF storage ring (Grenoble, France). Data (1500 successive 1 second frames), were collected using a Pilatus 1M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.09919 nm. The SEC mobile phase consisted of 20 mM Tris-HCl, 150 mM NaCl, pH 7.5, (20°C) with a sample injection concentration of 10 mg/ml. Data obtained from solute-free eluates and SEC-elution peak were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted from the SEC-peak frames to produce the data displayed in this entry.",
  "tags": [],
  "intensity_unit": null,
  "experimental_mw": 171.15,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 171.16,
  "porod_mw_error": null,
  "pddf_i0": 52.14,
  "pddf_i0_error": 0.04,
  "guinier_i0": 52.35,
  "guinier_i0_error": 0.048,
  "pddf_rg": 3.83,
  "pddf_rg_error": 0.003,
  "guinier_rg": 3.88,
  "guinier_rg_error": 0.01,
  "pddf_dmax": 9.99,
  "pddf_dmax_error": null,
  "porod_volume": 290.96,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 1,
  "guinier_point_last": 60,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDBH7_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
  "bragg_peak": []
}

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  "started_at": "2026-08-11T16:32:30.514552+00:00",
  "finished_at": "2026-08-11T16:32:39.146943+00:00",
  "source_last_modified": "2019-12-06T13:07:15.624532+01:00",
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