SASDBL2

MBP-PICK1 fusion

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2019-12-06T13:07:15.624532+01:00

This dataset was collected at a concentration of 3.75 mg/ml.

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · MBP-PICK1 fusion

Concentration0.46 – 7.5 Buffer / pH50 mM Tris 300 mM NaCl 1 mM maltose 1 mM EGTA 2 mM DTT / 7.5
Experimental temperature4.0 Facility / beamlineCornell High Energy Synchrotron Source (CHESS) / G1
Wavelength— nmExposure40.0 s × 20

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Maltose Binding Protein fused to Protein Interacting with C kinase 1
View sequence
MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDAALAAAQTNAAAMFADLDYDIEEDKLGIPTVPGKVTLQKDAQNLIGISIGGGAQYCPCLYIVQVFDNTPAALDGTVAAGDEITGVNGRSIKGKTKVEVAKMIQEVKGEVTIHYNKLQADPKQGMSLDIVLKKVKHRLVENMSSGTADALGLSRAILCNDGLVKRLEELERTAELYKGMTEHTKNLLRAFYELSQTHRAFGDVFSVIGVREPQPAASEAFVKFADAHRSIEKFGIRLLKTIKPMLTDLNTYLNKAIPDTRLTIKKYLDVKFEYLSYCLKVKEMDDEEYSCIALGEPLYRVSTGNYEYRLILRCRQEARARFSQMRKDVLEKMELLDQKHVQDIVFQLQRLVSTMSKYYNDCYAVLRDADVFPIEVDLAHTTLAYGLNQEEFTDGEEEEEEEDTAAGEPSRDTRGAAGPLDKGGSWCDS
proteinHomo sapiensQ9NRD5—–—dimerMolecule count 287.089 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1467[3]0.103426–2.93517 1/nmHas uncertainty columnMissing 079sasbdb/entries/l2/sasdbl2/source/SASDBL2.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)27.6—nm
i0Guinier41.42——
i0P(r)41.58——
mwExperimental172.2—kDa
porod_volumePorod483.0—nm³
rgGuinier8.38—nm
rgP(r)8.7—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name50 mM Tris 300 mM NaCl 1 mM maltose 1 mM EGTA 2 mM DTTBuffer concentration—
pH7.5Additives300 mM NaCl, 1 mM maltose, 1 mM EGTA, 2 mM DTT
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2015-10-13Storage / measurement temperature— / 4.0
Exposure time40.0Frames20
Wavelength—Sample-to-detector distance—
SourceX-ray synchrotronDetectorFinger Lakes CCD
Facility / beamlineCornell High Energy Synchrotron Source (CHESS) / G1 · Ithaca, NY, USA
q range0.103 – 2.935Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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full_entry_zip:source record and ZIP contents (4 items)
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:156 field values
Field pathRaw value
codeSASDBL2
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titlePICK1 is implicated in organelle motility in an Arp2/3 complex-independent manner.
project.publication.titlePICK1 is implicated in organelle motility in an Arp2/3 complex-independent manner.
project.publication.author_listMadasu Y, Yang C, Boczkowska M, Bethoney KA, Zwolak A, Rebowski G, Svitkina T, Dominguez R
project.publication.journalMol Biol Cell
project.publication.doi10.1091/mbc.E14-10-1448
project.publication.pmid25657323
project.publication.published_date2015 Apr 1
project.statusreleased
project.submitted_date2015-10-13
project.released_date2016-04-15
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDBL2.out
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pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBL2_pofr_img.png
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sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDBL2.sascif
experiment.instrument.detector.typeCCD
experiment.instrument.detector.nameFinger Lakes CCD
experiment.instrument.detector.resolution0.0
experiment.instrument.nameCornell High Energy Synchrotron Source (CHESS)
experiment.instrument.cityIthaca, NY
experiment.instrument.countryUSA
experiment.instrument.beamline_nameG1
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameMaltose Binding Protein fused to Protein Interacting with C kinase 1
experiment.sample.molecule[0].short_nameMBP-PICK1
experiment.sample.molecule[0].sequenceMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDI IFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNK DLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIK DVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSK VNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPL GAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDA ALAAAQTNAAAMFADLDYDIEEDKLGIPTVPGKVTLQKDAQNLIGISIGGGAQYCPCLYI VQVFDNTPAALDGTVAAGDEITGVNGRSIKGKTKVEVAKMIQEVKGEVTIHYNKLQADPK QGMSLDIVLKKVKHRLVENMSSGTADALGLSRAILCNDGLVKRLEELERTAELYKGMTEH TKNLLRAFYELSQTHRAFGDVFSVIGVREPQPAASEAFVKFADAHRSIEKFGIRLLKTIK PMLTDLNTYLNKAIPDTRLTIKKYLDVKFEYLSYCLKVKEMDDEEYSCIALGEPLYRVST GNYEYRLILRCRQEARARFSQMRKDVLEKMELLDQKHVQDIVFQLQRLVSTMSKYYNDCY AVLRDADVFPIEVDLAHTTLAYGLNQEEFTDGEEEEEEEDTAAGEPSRDTRGAAGPLDKG GSWCDS
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeQ9NRD5
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMFADLDYDIEEDKLGIPTVPGKVTLQKDAQNLIGISIGGGAQYCPCLYIVQVFDNTPAAL DGTVAAGDEITGVNGRSIKGKTKVEVAKMIQEVKGEVTIHYNKLQADPKQGMSLDIVLKK VKHRLVENMSSGTADALGLSRAILCNDGLVKRLEELERTAELYKGMTEHTKNLLRAFYEL SQTHRAFGDVFSVIGVREPQPAASEAFVKFADAHRSIEKFGIRLLKTIKPMLTDLNTYLN KAIPDTRLTIKKYLDVKFEYLSYCLKVKEMDDEEYSCIALGEPLYRVSTGNYEYRLILRC RQEARARFSQMRKDVLEKMELLDQKHVQDIVFQLQRLVSTMSKYYNDCYAVLRDADVFPI EVDLAHTTLAYGLNQEEFTDGEEEEEEEDTAAGEPSRDTRGAAGPLDKGGSWCDS
experiment.sample.molecule[0].mw87.089
experiment.sample.molecule[0].total_mw174.178
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name50 mM Tris 300 mM NaCl 1 mM maltose 1 mM EGTA 2 mM DTT
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive300 mM NaCl, 1 mM maltose, 1 mM EGTA, 2 mM DTT
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameMBP-PICK1 fusion
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namePenn
experiment.contributor[0].affiliation[0].addressPhiladelphia, PA, United States
experiment.contributor[0].affiliation[0].full_nameUniversity of Pennsylvania
experiment.contributor[0].affiliation[0].webpagehttp://www.upenn.edu/
experiment.contributor[0].contributor_nameRoberto
experiment.contributor[0].contributor_surnameDominguez
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2015-10-13
experiment.storage_temperaturenull
experiment.cell_temperature4.0
experiment.exposure_time40.0
experiment.number_of_frames20
experiment.wavelengthnull
experiment.sample_detector_distancenull
experiment.concentration_min0.46
experiment.concentration_max7.5
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.103
experiment.s_max2.935
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].softwareCRYSOL
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fits[0].models[0].lognull
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fits[0].models[0].comment
fits[0].models[0].user87
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fits[0].softwarenull
fits[0].chi_square_value1.1881
fits[0].p_value0.0
fits[0].fit_residual_plotfit4columns358_fitresiduals_img.png
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fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionThis dataset was collected at a concentration of 3.75 mg/ml.
experiment_descriptionnull
tags[]
intensity_unitnull
experimental_mw172.2
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i041.58
pddf_i0_errornull
guinier_i041.42
guinier_i0_errornull
pddf_rg8.7
pddf_rg_errornull
guinier_rg8.38
guinier_rg_errornull
pddf_dmax27.6
pddf_dmax_errornull
porod_volume483.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_firstnull
guinier_point_lastnull
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDBL2_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDBL2
statussuccess
started_at2026-08-11T13:40:45.692213+00:00
finished_at2026-08-11T13:40:55.673814+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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View the complete summary.json source
{
  "code": "SASDBL2",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "PICK1 is implicated in organelle motility in an Arp2/3 complex-independent manner.",
    "publication": {
      "title": "PICK1 is implicated in organelle motility in an Arp2/3 complex-independent manner.",
      "author_list": "Madasu Y, Yang C, Boczkowska M, Bethoney KA, Zwolak A, Rebowski G, Svitkina T, Dominguez R",
      "journal": "Mol Biol Cell",
      "doi": "10.1091/mbc.E14-10-1448",
      "pmid": "25657323",
      "published_date": "2015 Apr 1"
    },
    "status": "released",
    "submitted_date": "2015-10-13",
    "released_date": "2016-04-15"
  },
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        "name": "Finger Lakes CCD",
        "resolution": 0.0
      },
      "name": "Cornell High Energy Synchrotron Source (CHESS)",
      "city": "Ithaca, NY",
      "country": "USA",
      "beamline_name": "G1",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
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    },
    "sample": {
      "molecule": [
        {
          "long_name": "Maltose Binding Protein fused to Protein Interacting with C kinase 1",
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View the complete manifest.json source
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