N-terminal domain of Vaccinia virus A46 protein (1-83)

{
  "code": "SASDBL7",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Vaccinia Virus Immunomodulator A46: A Lipid and Protein-Binding Scaffold for Sequestering Host TIR-Domain Proteins.",
    "publication": {
      "title": "Vaccinia Virus Immunomodulator A46: A Lipid and Protein-Binding Scaffold for Sequestering Host TIR-Domain Proteins.",
      "author_list": "Fedosyuk S, Bezerra GA, Radakovics K, Smith TK, Sammito M, Bobik N, Round A, Ten Eyck LF, Djinović-Carugo K, Usón I, Skern T",
      "journal": "PLoS Pathog",
      "doi": "10.1371/journal.ppat.1006079",
      "pmid": "27973613",
      "published_date": "2016 Dec"
    },
    "status": "released",
    "submitted_date": "2016-11-06",
    "released_date": "2016-11-23"
  },
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  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBL7_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBL7_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBL7_guinier_img.png",
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  "experiment": {
    "instrument": {
      "detector": {
        "type": "Dectris",
        "name": "Pilatus 1M",
        "resolution": 172.0
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Protein A46",
          "short_name": "A46-delta",
          "sequence": "GSMAQQMAFDISVNASKTINALVYFSTQQNKLVIRNEVNDTHYTVEFDRDKVVDTFISYNRHNDTIEIRGVLPEET\r\nNIGCAVNTPVSMT",
          "organism": "Vaccinia virus",
          "uniprot_code": "P26672",
          "uniprot_range_first": 1,
          "uniprot_range_last": 83,
          "oligomerization": "tetramer",
          "molecular_type": "protein",
          "uniprot_sequence": "MAFDISVNASKTINALVYFSTQQNKLVIRNEVNDTHYTVEFDRDKVVDTFISYNRHNDTI\nEIRGVLPEETNIGCAVNTPVSMTYLYNKYSFKLILAEYIRHRNTISGNIYSALMTLDDLA\nIKQYGDIDLLFNEKLKVDSDSGLFDFVNFVKDMICCDSRIVVALSSLVSKHWELTNKKYR\nCMALAEHISDSIPISELSRLRYNLCKYLRGHTESIEDKFDYFEDDDSSTCSAVTDRETDV",
          "mw": 10.028,
          "total_mw": 40.112,
          "number_molecules": 4,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "20 mM Tris-HCl, 10 mM DTT",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "N-terminal domain of Vaccinia virus A46 protein (1-83)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Vienna, Austria",
            "full_name": "Medical University of Vienna",
            "webpage": "https://www.meduniwien.ac.at"
          }
        ],
        "contributor_name": "Sofiya",
        "contributor_surname": "Fedosyuk",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2015-06-25",
    "storage_temperature": null,
    "cell_temperature": 20.0,
    "exposure_time": 0.1,
    "number_of_frames": null,
    "wavelength": 0.09918,
    "sample_detector_distance": 2.867,
    "concentration_min": null,
    "concentration_max": 15.5,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.131,
    "s_max": 4.934,
    "total_exposure_time": null,
    "seccolumn": null
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              "pdb_code": "5ezu",
              "ext_code": "5EZU",
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              "difference_with_model": "derived"
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          "bead_radius": 1.9,
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          "user": 176
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      "chi_square_value": 0.657,
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  "pddf_software_version": "GNOM v5",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data, I(s) vs s (s = 4π sin θ/λ, where 2θ is the scattering angle) were collected from a sample of the N-terminal domain of Vaccinia virus A46 protein (1-83) using continuous-flow size-exclusion chromatography SAXS (SEC-SAXS; Superdex 200 10/300 column) at the BM29 beam line on the ESRF storage ring (Grenoble, France). Data were collected using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09918 nm. The SEC mobile phase consisted of 20 mM Tris-HCl, 10 mM DTT, pH 8.5, (20°C) with a sample injection concentration of 15.5 mg/ml. Data obtained from solute-free eluates and SEC-elution peak were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted from the SEC-peak frames to produce the data displayed in this entry.",
  "tags": [],
  "intensity_unit": null,
  "experimental_mw": 38.857,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 38.857,
  "porod_mw_error": null,
  "pddf_i0": 14.97,
  "pddf_i0_error": 0.03,
  "guinier_i0": 14.91,
  "guinier_i0_error": 0.02,
  "pddf_rg": 2.65,
  "pddf_rg_error": 0.01,
  "guinier_rg": 2.61,
  "guinier_rg_error": 0.09,
  "pddf_dmax": 9.0,
  "pddf_dmax_error": null,
  "porod_volume": 68.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 1,
  "guinier_point_last": 65,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDBL7_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
  "bragg_peak": []
}

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  "source_last_modified": "2019-12-06T13:07:15.624532+01:00",
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