SASDBN3

Highly similar to Actin cross-linking family protein 7 (ACF7) Y259D mutant Homo Sapiens

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2023-03-02T16:54:16.648202+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Highly similar to Actin cross-linking family protein 7 (ACF7) Y259D mutant Homo Sapiens

Concentration— – 0.97 Buffer / pHPBS / 7.4
Experimental temperature— Facility / beamlineAdvanced Photon Source (APS), Argonne National Laboratory / 12-ID-B SAXS/WAXS
Wavelength— nmExposure1.0 s × 30

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 Y259D mutant
View sequence
MFPVLWAGIPGRDVGSLQPLPPGFKQFCTSASRVAVIADERDRVQKKTFTKWVNKHLMKVRKHINDLYEDLRDGHNLISLLEVLSGIKLPREKGRMRFHRLQNVQIALDFLKQRQVKLVNIRNDDITDGNPKLTLGLIWTIILHFQISDIYISGESGDMSAKEKLLLWTQKVTAGYTGIKCTNFSSCWSDGKMFNALIHRYRPDLVDMERVQIQSNRENLEQAFEVAERLGVTRLLDAEDVDVPSPDEKSVITYVSSIDDAFPKVPEGGEGISATEVDSRWQEYQSRVDSLIPWIKQHTILMSDKTFPQNPVELKALYNQYIHFKETEILAKEREKGRIEELYKLLEVWIEFGRIKLPQGYHPNDVEEEWGKLIIEMLEREKSLRPAVERLELLLQIANK
proteinHomo sapiensQ6ZSD71–400monomerMolecule count 146.294 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1273[3]0.20255–10.04885 1/nmHas uncertainty columnMissing 00sasbdb/entries/n3/sasdbn3/source/SASDBN3.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)13.5—nm
i0Guinier0.0940.001—
i0P(r)0.0970.004—
mwExperimental44.0—kDa
mwPorod34.5—kDa
porod_volumePorod55.2—nm³
rgGuinier3.380.12nm
rgP(r)3.680.18nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer namePBSBuffer concentration—
pH7.4Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2014-11-07Storage / measurement temperature25.0 / —
Exposure time1.0Frames30
Wavelength—Sample-to-detector distance—
SourceX-ray synchrotronDetectorPilatus 2M
Facility / beamlineAdvanced Photon Source (APS), Argonne National Laboratory / 12-ID-B SAXS/WAXS · Lemont, IL, USA
q range0.203 – 10.049Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:134 field values
Field pathRaw value
codeSASDBN3
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleIn vivo epidermal migration requires focal adhesion targeting of ACF7.
project.publication.titleIn vivo epidermal migration requires focal adhesion targeting of ACF7.
project.publication.author_listYue J, Zhang Y, Liang WG, Gou X, Lee P, Liu H, Lyu W, Tang WJ, Chen SY, Yang F, Liang H, Wu X
project.publication.journalNat Commun
project.publication.doi10.1038/ncomms11692
project.publication.pmid27216888
project.publication.published_date2016 May 24
project.statusreleased
project.submitted_date2016-01-26
project.released_date2016-09-19
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDBN3.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDBN3.dat
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intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBN3_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBN3_pofr_img.png
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.nameAdvanced Photon Source (APS), Argonne National Laboratory
experiment.instrument.cityLemont, IL
experiment.instrument.countryUSA
experiment.instrument.beamline_name12-ID-B SAXS/WAXS
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_namecDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 Y259D mutant
experiment.sample.molecule[0].short_nameACF7-Y259D
experiment.sample.molecule[0].sequenceMFPVLWAGIPGRDVGSLQPLPPGFKQFCTSASRVAVIADERDRVQKKTFTKWVNKHLMKVRKHINDLYEDLRDGHNLISLLEVLSGIKLPREKGRMRFHRLQNVQIALDFLKQRQVKLVNIRNDDITDGNPKLTLGLIWTIILHFQISDIYISGESGDMSAKEKLLLWTQKVTAGYTGIKCTNFSSCWSDGKMFNALIHRYRPDLVDMERVQIQSNRENLEQAFEVAERLGVTRLLDAEDVDVPSPDEKSVITYVSSIDDAFPKVPEGGEGISATEVDSRWQEYQSRVDSLIPWIKQHTILMSDKTFPQNPVELKALYNQYIHFKETEILAKEREKGRIEELYKLLEVWIEFGRIKLPQGYHPNDVEEEWGKLIIEMLEREKSLRPAVERLELLLQIANK
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeQ6ZSD7
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last400
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMGNSLGCFKEPKESIAIPEKAPISPKKRVRFKRRWRGKKIPTPEASHQEETSEGTGVIEE TETLTKLTESLQKEDGVGGVEHTPPDILLPGDSAPNSRVVDRGMIVQVKERFQGEVQTAH LLLENESSVAGGVWDSLEEGMTVIAHLLDNPAERNCEKSVSQLVEFPRTASCSSRAVLLP LQGETAVEKGNIQRGFRSCALPRTDYPTDKGNQEQFSEGWSVEEGTKSVSGAPQTASWII ECSVSSLLLDQPGGQRRTEPSHVGQVPPQDSRLPTSQSDLSISGVTVSILPSSSGYGSDG PHIHGIQPKDTEPEKSSTSFSEEDGTLSLEASHTPSWGLEEISDIYISGESGDMSAKEKL LLWTQKVTAGYTGIKCTNFSSCWSDGKMFNALIHRYRPDLVDMERVQIQSNRENLEQAFE VAERLGVTRLLDAEDVDVPSPDEKSVITYVSSIYDAFPKVPEGGEGISAMEVDSRWQEYQ SRVDSLIPWIKQHTILMSDKTFPQNPVELKALYNQYIHFKETEILAKEREKGRIEELYKL LEVWIEFGRIKLPQGYHPNDVEEEWGKLIIEMLEREKSLRPAVERLELLLQIANKIQNGA LNCEEKLTLAKNTLQADAAHLESGQPVQCESDVIMYIQECEGLIRQLQVDLQILRDENYY QLEELAFRVMRLQDELVTLRLECTNLYRKGHFTSLELVPPSTLTTTHLKAEPLTKATHSS STSWFRKPMTRAELVAISSSEDEGNLRFVYELLSWVEEMQMKLERAEWGNDLLSVELQLE TQPHIHTSVEELGSSVKEARLYEGKMSQNFHTSYAETLGKLETQYCKLKETSSFRMRHLQ SLHKFVSRATAELIWLNEKEEEELAYDWSDNNSNISAKRNYFSELTMELEEKQDVFRSLQ DTAELLSLENHPAKQTVEAYSAAVQSQLQWMKQPCLCVEQHVKENTAYFQFFSDARELES FLRNLQDSIKRKYSCDHNTSLSRLEDLLQDSMAQDEKEQLIQSKSSVASLVGRSKTIVQL KPRSPDHVLKNTISVKAVCDYRQIEGRTILSEGYGPLASAAC
experiment.sample.molecule[0].mw46.294
experiment.sample.molecule[0].total_mw46.294
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.namePBS
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameHighly similar to Actin cross-linking family protein 7 (ACF7) Y259D mutant Homo Sapiens
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameUchicago
experiment.contributor[0].affiliation[0].addressEdward H. Levi Hall 5801 South Ellis Avenue Chicago, Illinois 60637 773.702.1234
experiment.contributor[0].affiliation[0].full_nameThe University of Chicago
experiment.contributor[0].affiliation[0].webpagehttp://www.uchicago.edu/
experiment.contributor[0].contributor_nameWenguang
experiment.contributor[0].contributor_surnameLiang
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2014-11-07
experiment.storage_temperature25.0
experiment.cell_temperaturenull
experiment.exposure_time1.0
experiment.number_of_frames30
experiment.wavelengthnull
experiment.sample_detector_distancenull
experiment.concentration_minnull
experiment.concentration_max0.97
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.203
experiment.s_max10.049
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron X-ray scattering data from solutions of the N-terminal region of Human ACF7-Y259D mutant in PBS, pH 7.4 were collected on the 12ID-B SAXS/WAXS beam line of the Advanced Photon Source (Argonne National Laboratory, IL) using a Pilatus 2M detector (I(s) vs s, where s = 4π sin θ/λ, and 2θ is the scattering angle;λ = 0.0886 nm). One solute concentration of 0.97 mg/ml was measured. The SAXS data from 30 successive 1 s frames were normalized to the intensity of the transmitted beam and radially averaged and the scattering of the solvent-blank was subtracted.
tags[]
intensity_unitnull
experimental_mw44.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw34.5
porod_mw_errornull
pddf_i00.097
pddf_i0_error0.004
guinier_i00.094
guinier_i0_error0.001
pddf_rg3.68
pddf_rg_error0.18
guinier_rg3.38
guinier_rg_error0.12
pddf_dmax13.5
pddf_dmax_errornull
porod_volume55.2
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first9
guinier_point_last32
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDBN3_datloglog_img.png
symmetrynull
last_modified2023-03-02T16:54:16.648202+01:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDBN3
statussuccess
started_at2026-08-11T14:03:53.099234+00:00
finished_at2026-08-11T14:03:59.941224+00:00
source_last_modified2023-03-02T16:54:16.648202+01:00
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View the complete summary.json source
{
  "code": "SASDBN3",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "In vivo epidermal migration requires focal adhesion targeting of ACF7.",
    "publication": {
      "title": "In vivo epidermal migration requires focal adhesion targeting of ACF7.",
      "author_list": "Yue J, Zhang Y, Liang WG, Gou X, Lee P, Liu H, Lyu W, Tang WJ, Chen SY, Yang F, Liang H, Wu X",
      "journal": "Nat Commun",
      "doi": "10.1038/ncomms11692",
      "pmid": "27216888",
      "published_date": "2016 May 24"
    },
    "status": "released",
    "submitted_date": "2016-01-26",
    "released_date": "2016-09-19"
  },
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        "name": "Pilatus 2M",
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      },
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      "city": "Lemont, IL",
      "country": "USA",
      "beamline_name": "12-ID-B SAXS/WAXS",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
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    "sample": {
      "molecule": [
        {
          "long_name": "cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 Y259D mutant",
          "short_name": "ACF7-Y259D",
          "sequence": "MFPVLWAGIPGRDVGSLQPLPPGFKQFCTSASRVAVIADERDRVQKKTFTKWVNKHLMKVRKHINDLYEDLRDGHNLISLLEVLSGIKLPREKGRMRFHRLQNVQIALDFLKQRQVKLVNIRNDDITDGNPKLTLGLIWTIILHFQISDIYISGESGDMSAKEKLLLWTQKVTAGYTGIKCTNFSSCWSDGKMFNALIHRYRPDLVDMERVQIQSNRENLEQAFEVAERLGVTRLLDAEDVDVPSPDEKSVITYVSSIDDAFPKVPEGGEGISATEVDSRWQEYQSRVDSLIPWIKQHTILMSDKTFPQNPVELKALYNQYIHFKETEILAKEREKGRIEELYKLLEVWIEFGRIKLPQGYHPNDVEEEWGKLIIEMLEREKSLRPAVERLELLLQIANK",
          "organism": "Homo sapiens",
          "uniprot_code": "Q6ZSD7",
          "uniprot_range_first": 1,
          "uniprot_range_last": 400,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MGNSLGCFKEPKESIAIPEKAPISPKKRVRFKRRWRGKKIPTPEASHQEETSEGTGVIEE\nTETLTKLTESLQKEDGVGGVEHTPPDILLPGDSAPNSRVVDRGMIVQVKERFQGEVQTAH\nLLLENESSVAGGVWDSLEEGMTVIAHLLDNPAERNCEKSVSQLVEFPRTASCSSRAVLLP\nLQGETAVEKGNIQRGFRSCALPRTDYPTDKGNQEQFSEGWSVEEGTKSVSGAPQTASWII\nECSVSSLLLDQPGGQRRTEPSHVGQVPPQDSRLPTSQSDLSISGVTVSILPSSSGYGSDG\nPHIHGIQPKDTEPEKSSTSFSEEDGTLSLEASHTPSWGLEEISDIYISGESGDMSAKEKL\nLLWTQKVTAGYTGIKCTNFSSCWSDGKMFNALIHRYRPDLVDMERVQIQSNRENLEQAFE\nVAERLGVTRLLDAEDVDVPSPDEKSVITYVSSIYDAFPKVPEGGEGISAMEVDSRWQEYQ\nSRVDSLIPWIKQHTILMSDKTFPQNPVELKALYNQYIHFKETEILAKEREKGRIEELYKL\nLEVWIEFGRIKLPQGYHPNDVEEEWGKLIIEMLEREKSLRPAVERLELLLQIANKIQNGA\nLNCEEKLTLAKNTLQADAAHLESGQPVQCESDVIMYIQECEGLIRQLQVDLQILRDENYY\nQLEELAFRVMRLQDELVTLRLECTNLYRKGHFTSLELVPPSTLTTTHLKAEPLTKATHSS\nSTSWFRKPMTRAELVAISSSEDEGNLRFVYELLSWVEEMQMKLERAEWGNDLLSVELQLE\nTQPHIHTSVEELGSSVKEARLYEGKMSQNFHTSYAETLGKLETQYCKLKETSSFRMRHLQ\nSLHKFVSRATAELIWLNEKEEEELAYDWSDNNSNISAKRNYFSELTMELEEKQDVFRSLQ\nDTAELLSLENHPAKQTVEAYSAAVQSQLQWMKQPCLCVEQHVKENTAYFQFFSDARELES\nFLRNLQDSIKRKYSCDHNTSLSRLEDLLQDSMAQDEKEQLIQSKSSVASLVGRSKTIVQL\nKPRSPDHVLKNTISVKAVCDYRQIEGRTILSEGYGPLASAAC",
          "mw": 46.294,
          "total_mw": 46.294,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "PBS",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.4,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Highly similar to Actin cross-linking family protein 7 (ACF7) Y259D mutant Homo Sapiens",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "Uchicago",
            "address": "Edward H. Levi Hall 5801 South Ellis Avenue Chicago, Illinois 60637 773.702.1234",
            "full_name": "The University of Chicago",
            "webpage": "http://www.uchicago.edu/"
          }
        ],
        "contributor_name": "Wenguang",
        "contributor_surname": "Liang",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2014-11-07",
    "storage_temperature": 25.0,
    "cell_temperature": null,
    "exposure_time": 1.0,
    "number_of_frames": 30,
    "wavelength": null,
    "sample_detector_distance": null,
    "concentration_min": null,
    "concentration_max": 0.97,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.203,
    "s_max": 10.049,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": null,
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron X-ray scattering data from solutions of the N-terminal region of Human ACF7-Y259D mutant in PBS, pH 7.4 were collected on the 12ID-B SAXS/WAXS beam line of the Advanced Photon Source (Argonne National Laboratory, IL) using a Pilatus 2M detector (I(s) vs s, where s = 4π sin θ/λ, and 2θ is the scattering angle;λ = 0.0886 nm). One solute concentration of 0.97 mg/ml was measured. The SAXS data from 30 successive 1 s frames were normalized to the intensity of the transmitted beam and radially averaged and the scattering of the solvent-blank was subtracted.",
  "tags": [],
  "intensity_unit": null,
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  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 34.5,
  "porod_mw_error": null,
  "pddf_i0": 0.097,
  "pddf_i0_error": 0.004,
  "guinier_i0": 0.094,
  "guinier_i0_error": 0.001,
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  "guinier_rg_error": 0.12,
  "pddf_dmax": 13.5,
  "pddf_dmax_error": null,
  "porod_volume": 55.2,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 9,
  "guinier_point_last": 32,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDBN3_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2023-03-02T16:54:16.648202+01:00",
  "bragg_peak": []
}
View the complete manifest.json source
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