SASDBP6

Nucleolysin TIA-1 isoform p40 in complex with ACTCCTTTTT DNA

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2020-07-22T10:39:57.780614+02:00

TIA-1 RRM23 + CT-rich DNA

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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Nucleolysin TIA-1 isoform p40 in complex with ACTCCTTTTT DNA

浓度— – 2.5 缓冲液 / pH20 mM HEPES, 100 mM NaCl, 3% v/v glycerol / 7.0
实验温度15.0 设备 / 束线Australian Synchrotron / SAXS/WAXS
波长0.103 nm曝光1.0 s × 30

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Nucleolysin TIA-1 isoform p40
查看序列
ENLYFQGAMGNHFHVFVGDLSPEITTEDIKAAFAPFGRISDARVVKDMATGKSKGYGFVSFFNKWDAENAIQQMGGQWLGGRQIRTNWATRKPPAPKSTYESNTKQLSYDEVVNQSSPSNCTVYCGGVTSGLTEQLMRQTFSPFGQIMEIRVFPDKGYSFVRFNSHESAAHAIVSVNGTTIEGHVVKCYWGK
proteinHomo sapiensP3148393–274monomer分子数 121.325 kDa
DNA (ACTCCTTTTT)
查看序列
ACTCCTTTTT
DNA———–—monomer分子数 10.702 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1912[3]0.0064398–0.361515 1/A含误差列缺失 02sasbdb/entries/p6/sasdbp6/source/SASDBP6.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)6.7—Å
i0Guinier0.0590.00016—
i0P(r)0.05820.0009—
mwExperimental23.1—kDa
porod_volumePorod32.0—ų
rgGuinier2.240.02Å
rgP(r)2.19—Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM HEPES, 100 mM NaCl, 3% v/v glycerol缓冲液浓度—
pH7.0添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2016-05-27储存 / 测量温度15.0 / 15.0
曝光时间1.0帧数30
波长0.103样品-探测器距离1.6
光源X-ray synchrotron探测器Pilatus 1M
机构 / 束线Australian Synchrotron / SAXS/WAXS · Melbourne, Australia
q 范围0.064 – 3.615样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:151 个字段值
字段路径原始值
codeSASDBP6
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleTIA-1 RRM23 binding and recognition of target oligonucleotides.
project.publication.titleTIA-1 RRM23 binding and recognition of target oligonucleotides.
project.publication.author_listWaris S, García-Mauriño SM, Sivakumaran A, Beckham SA, Loughlin FE, Gorospe M, Díaz-Moreno I, Wilce MCJ, Wilce JA
project.publication.journalNucleic Acids Res
project.publication.doi10.1093/nar/gkx102
project.publication.pmid28184449
project.publication.published_date2017 May 5
project.statusreleased
project.submitted_date2016-09-22
project.released_date2017-03-28
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDBP6.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDBP6.dat
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sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDBP6.sascif
experiment.instrument.detector.typeDectris
experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameAustralian Synchrotron
experiment.instrument.cityMelbourne
experiment.instrument.countryAustralia
experiment.instrument.beamline_nameSAXS/WAXS
experiment.instrument.beam_geometryPoint
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourceTrue
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameNucleolysin TIA-1 isoform p40
experiment.sample.molecule[0].short_nameTIA-1
experiment.sample.molecule[0].sequenceENLYFQGAMGNHFHVFVGDLSPEITTEDIKAAFAPFGRISDARVVKDMATGKSKGYGFVSFFNKWDAENAIQQMGGQWLGGRQIRTNWATRKPPAPKSTYESNTKQLSYDEVVNQSSPSNCTVYCGGVTSGLTEQLMRQTFSPFGQIMEIRVFPDKGYSFVRFNSHESAAHAIVSVNGTTIEGHVVKCYWGK
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP31483
experiment.sample.molecule[0].uniprot_range_first93
experiment.sample.molecule[0].uniprot_range_last274
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMEDEMPKTLYVGNLSRDVTEALILQLFSQIGPCKNCKMIMDTAGNDPYCFVEFHEHRHAA AALAAMNGRKIMGKEVKVNWATTPSSQKKDTSSSTVVSTQRSQDHFHVFVGDLSPEITTE DIKAAFAPFGRISDARVVKDMATGKSKGYGFVSFFNKWDAENAIQQMGGQWLGGRQIRTN WATRKPPAPKSTYESNTKQLSYDEVVNQSSPSNCTVYCGGVTSGLTEQLMRQTFSPFGQI MEIRVFPDKGYSFVRFNSHESAAHAIVSVNGTTIEGHVVKCYWGKETLDMINPVQQQNQI GYPQPYGQWGQWYGNAQQIGQYMPNGWQVPAYGMYGQAWNQQGFNQTQSSAPWMGPNYGV QPPQGQNGSMLPNQPSGYRVAGYETQ
experiment.sample.molecule[0].mw21.325
experiment.sample.molecule[0].total_mw21.325
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_nameDNA (ACTCCTTTTT)
experiment.sample.molecule[1].short_namenull
experiment.sample.molecule[1].sequenceACTCCTTTTT
experiment.sample.molecule[1].organismnull
experiment.sample.molecule[1].uniprot_codenull
experiment.sample.molecule[1].uniprot_range_firstnull
experiment.sample.molecule[1].uniprot_range_lastnull
experiment.sample.molecule[1].oligomerizationmonomer
experiment.sample.molecule[1].molecular_typeDNA
experiment.sample.molecule[1].uniprot_sequencenull
experiment.sample.molecule[1].mw0.702
experiment.sample.molecule[1].total_mw0.702
experiment.sample.molecule[1].number_molecules1
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
experiment.sample.buffer.name20 mM HEPES, 100 mM NaCl, 3% v/v glycerol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameNucleolysin TIA-1 isoform p40 in complex with ACTCCTTTTT DNA
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressMelbourne, Australia
experiment.contributor[0].affiliation[0].full_nameMonash University
experiment.contributor[0].affiliation[0].webpagehttp://www.monash.edu/
experiment.contributor[0].contributor_nameMatthew
experiment.contributor[0].contributor_surnameWilce
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2016-05-27
experiment.storage_temperature15.0
experiment.cell_temperature15.0
experiment.exposure_time1.0
experiment.number_of_frames30
experiment.wavelength0.103
experiment.sample_detector_distance1.6
experiment.concentration_minnull
experiment.concentration_max2.5
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.064
experiment.s_max3.615
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionTIA-1 RRM23 + CT-rich DNA
experiment_descriptionSynchrotron SAXS data from solutions of Nucleolysin TIA-1 isoform p40 in complex with DNA (ACTCCTTTTT) in 20 mM HEPES, 100mM NaCl, 3% v/v glycerol, pH 7 were collected on the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 1.6 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). The data were collected as 30 successive 1 second frames and were normalized to the intensity of the transmitted beam and radially averaged. The radially-averaged scattering of the solvent-blank was subtracted to produce the results displayed in this entry that shows the scattering profile from a sample at 2.5 mg/ml maintained at 15°C during the SAXS measurements.
tags[]
intensity_unitnull
experimental_mw23.1
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i00.0582
pddf_i0_error0.0009
guinier_i00.059
guinier_i0_error0.00016
pddf_rg2.19
pddf_rg_errornull
guinier_rg2.24
guinier_rg_error0.02
pddf_dmax6.7
pddf_dmax_errornull
porod_volume32.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first32
guinier_point_last96
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDBP6_datloglog_img.png
symmetrynull
last_modified2020-07-22T10:39:57.780614+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDBP6
statussuccess
started_at2026-08-11T16:43:35.150764+00:00
finished_at2026-08-11T16:43:43.369678+00:00
source_last_modified2020-07-22T10:39:57.780614+02:00
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查看完整 summary.json 原文
{
  "code": "SASDBP6",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "TIA-1 RRM23 binding and recognition of target oligonucleotides.",
    "publication": {
      "title": "TIA-1 RRM23 binding and recognition of target oligonucleotides.",
      "author_list": "Waris S, García-Mauriño SM, Sivakumaran A, Beckham SA, Loughlin FE, Gorospe M, Díaz-Moreno I, Wilce MCJ, Wilce JA",
      "journal": "Nucleic Acids Res",
      "doi": "10.1093/nar/gkx102",
      "pmid": "28184449",
      "published_date": "2017 May 5"
    },
    "status": "released",
    "submitted_date": "2016-09-22",
    "released_date": "2017-03-28"
  },
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  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDBP6.dat",
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      },
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      "country": "Australia",
      "beamline_name": "SAXS/WAXS",
      "beam_geometry": "Point",
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          "long_name": "Nucleolysin TIA-1 isoform p40",
          "short_name": "TIA-1",
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        },
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        "name": "20 mM HEPES, 100 mM NaCl, 3% v/v glycerol",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.0,
        "deuteration": null
      },
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      "name": "Nucleolysin TIA-1 isoform p40 in complex with ACTCCTTTTT DNA",
      "ext_coefficient": null,
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      "absorbption": null,
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    },
    "contributor": [
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        "affiliation": [
          {
            "short_name": null,
            "address": "Melbourne, Australia",
            "full_name": "Monash University",
            "webpage": "http://www.monash.edu/"
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        "contributor_name": "Matthew",
        "contributor_surname": "Wilce",
        "orcid": null
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    "concentration_method": null,
    "concentration_unit": null,
    "date": "2016-05-27",
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    "cell_temperature": 15.0,
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    "wavelength": 0.103,
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  "pddf_software_version": null,
  "i0_calibration_standard": null,
  "description": "TIA-1 RRM23 + CT-rich DNA",
  "experiment_description": "Synchrotron SAXS data from solutions of Nucleolysin TIA-1 isoform p40 in complex with DNA (ACTCCTTTTT) in 20 mM HEPES, 100mM NaCl, 3% v/v glycerol, pH 7 were collected on the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 1.6 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). The data were collected as 30 successive 1 second frames and were normalized to the intensity of the transmitted beam and radially averaged. The radially-averaged scattering of the solvent-blank was subtracted to produce the results displayed in this entry that shows the scattering profile from a sample at 2.5 mg/ml maintained at 15°C during the SAXS measurements.",
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  "guinier_point_first": 32,
  "guinier_point_last": 96,
  "pddf_point_first": null,
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  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDBP6_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2020-07-22T10:39:57.780614+02:00",
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}
查看完整 manifest.json 原文
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