SASDBQ7

Domain 3'X of hepatitis C virus RNA at higher ionic strength

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Extrapolated to infinite dilution 最后更新:2024-09-11T17:08:26.026510+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Domain 3'X of hepatitis C virus RNA at higher ionic strength

浓度0.37 – 1.5 缓冲液 / pH10mM Tris 0.1 mM EDTA 2 mM MgCl2 50 mM NaCl / 7.0
实验温度27.0 设备 / 束线Center for Cancer Research, National Cancer Institute / Rigaku BioSAXS-2000
波长0.154 nm曝光1.0 s × 30

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Domain 3'X of hepatitis C virus
查看序列
GGUGGCUCCAUCUUAGCCCUAGUCACGGCUAGCUGUGAAAGGUCCGUGAGCCGCUUGACUGCAGAGAGUGCUGAUACUGGCCUCUCUGCAGAUCAAGU
RNAHepatitis C virus——–—dimer分子数 231.7 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1501[3]0.0–1.0 1/A含误差列缺失 0159sasbdb/entries/q7/sasdbq7/source/SASDBQ7.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)24.40.06Å
i0Guinier89.690.2—
i0P(r)91.70.0—
mwExperimental81.9—kDa
rgGuinier5.9920.022Å
rgP(r)6.4770.0Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称10mM Tris 0.1 mM EDTA 2 mM MgCl2 50 mM NaCl缓冲液浓度—
pH7.0添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2015-11-20储存 / 测量温度27.0 / 27.0
曝光时间1.0帧数30
波长0.154样品-探测器距离0.5
光源X-ray in house探测器Pilatus 100K
机构 / 束线Center for Cancer Research, National Cancer Institute / Rigaku BioSAXS-2000 · Frederick, MD, USA
q 范围0.0 – 10.0样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDBQ7
statusPublished
type_of_curveExtrapolated to infinite dilution
angular_unit1/A
project.titleThree-dimensional structure of the 3'X-tail of hepatitis C virus RNA in monomeric and dimeric states.
project.publication.titleThree-dimensional structure of the 3'X-tail of hepatitis C virus RNA in monomeric and dimeric states.
project.publication.author_listCantero-Camacho Á, Fan L, Wang YX, Gallego J
project.publication.journalRNA
project.publication.doi10.1261/rna.060632.117
project.publication.pmid28630140
project.publication.published_date2017 Sep
project.statusreleased
project.submitted_date2016-11-23
project.released_date2017-06-26
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experiment.instrument.nameCenter for Cancer Research, National Cancer Institute
experiment.instrument.cityFrederick, MD
experiment.instrument.countryUSA
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experiment.sample.molecule[0].short_name3'X
experiment.sample.molecule[0].sequenceGGUGGCUCCAUCUUAGCCCUAGUCACGGCUAGCUGUGAAAGGUCCGUGAGCCGCUUGACUGCAGAGAGUGCUGAUACUGGCCUCUCUGCAGAUCAAGU
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experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressValencia, Spain
experiment.contributor[0].affiliation[0].full_nameUniversidad Católica de Valencia
experiment.contributor[0].affiliation[0].webpagehttps://www.ucv.es/
experiment.contributor[0].contributor_nameJosé
experiment.contributor[0].contributor_surnameGallego
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experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2015-11-20
experiment.storage_temperature27.0
experiment.cell_temperature27.0
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pddf_softwareATSAS GNOM
pddf_software_versionGNOM 4.6
i0_calibration_standardnull
descriptionnull
experiment_descriptionSAXS data from solutions of Domain 3'X of hepatitis C virus RNA at higher ionic strength in 10mM Tris 0.1 mM EDTA 2 mM MgCl2 50 mM NaCl, pH 7, were collected on an in-house Rigaku BIOSAXS-2000 instrument (Frederick, USA) using a Pilatus 100K detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). Solute concentrations ranging between 0.4 and 1.5 mg/ml were measured at 27°C. 30 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for RNA concentration. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
tags[]
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pddf_i091.7
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symmetrynull
last_modified2024-09-11T17:08:26.026510+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDBQ7
statussuccess
started_at2026-08-11T16:41:14.793331+00:00
finished_at2026-08-11T16:41:22.526814+00:00
source_last_modified2024-09-11T17:08:26.026510+02:00
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查看完整 summary.json 原文
{
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  "status": "Published",
  "type_of_curve": "Extrapolated to infinite dilution",
  "angular_unit": "1/A",
  "project": {
    "title": "Three-dimensional structure of the 3'X-tail of hepatitis C virus RNA in monomeric and dimeric states.",
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      "title": "Three-dimensional structure of the 3'X-tail of hepatitis C virus RNA in monomeric and dimeric states.",
      "author_list": "Cantero-Camacho Á, Fan L, Wang YX, Gallego J",
      "journal": "RNA",
      "doi": "10.1261/rna.060632.117",
      "pmid": "28630140",
      "published_date": "2017 Sep"
    },
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}
查看完整 manifest.json 原文
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