SASDBR7

Subdomain SL2' of hepatitis C virus RNA domain 3'X at higher ionic strength

Data type: SASBDB experimental data Status: Published Curve type: Extrapolated to infinite dilution Last updated: 2023-03-02T16:54:16.648202+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Subdomain SL2' of hepatitis C virus RNA domain 3'X at higher ionic strength

Concentration0.5 – 2.0 Buffer / pH10mM Tris 0,1mM EDTA 2mM MgCl2 / 7.0
Experimental temperature27.0 Facility / beamlineAdvanced Photon Source (APS), Argonne National Laboratory / 12-ID-B SAXS/WAXS
Wavelength0.0886 nmExposure1.0 s × 30

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Subdomain SL2' of hepatitis C virus domain 3'X
View sequence
GGUGGCUCCAUCUUAGCCCUAGUCACGGCUAGCUGUGAAAGGUCCGUGAGCCGCU
RNAHepatitis C virus——–—dimerMolecule count 217.8 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1501[3]0.0–1.0 1/AHas uncertainty columnMissing 05sasbdb/entries/r7/sasdbr7/source/SASDBR7.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)16.90.06Å
i0Guinier0.140.0004—
i0P(r)0.14060.0—
mwExperimental40.1—kDa
rgGuinier4.1060.025Å
rgP(r)4.360.0Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name10mM Tris 0,1mM EDTA 2mM MgCl2Buffer concentration—
pH7.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2015-11-11Storage / measurement temperature27.0 / 27.0
Exposure time1.0Frames30
Wavelength0.0886Sample-to-detector distance2.0
SourceX-ray synchrotronDetectorPilatus 2M
Facility / beamlineAdvanced Photon Source (APS), Argonne National Laboratory / 12-ID-B SAXS/WAXS · Lemont, IL, USA
q range0.0 – 10.0Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:156 field values
Field pathRaw value
codeSASDBR7
statusPublished
type_of_curveExtrapolated to infinite dilution
angular_unit1/A
project.titleThree-dimensional structure of the 3'X-tail of hepatitis C virus RNA in monomeric and dimeric states.
project.publication.titleThree-dimensional structure of the 3'X-tail of hepatitis C virus RNA in monomeric and dimeric states.
project.publication.author_listCantero-Camacho Á, Fan L, Wang YX, Gallego J
project.publication.journalRNA
project.publication.doi10.1261/rna.060632.117
project.publication.pmid28630140
project.publication.published_date2017 Sep
project.statusreleased
project.submitted_date2016-11-23
project.released_date2017-06-26
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experiment.instrument.detector.resolutionnull
experiment.instrument.nameAdvanced Photon Source (APS), Argonne National Laboratory
experiment.instrument.cityLemont, IL
experiment.instrument.countryUSA
experiment.instrument.beamline_name12-ID-B SAXS/WAXS
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
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experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameSubdomain SL2' of hepatitis C virus domain 3'X
experiment.sample.molecule[0].short_nameSL2'
experiment.sample.molecule[0].sequenceGGUGGCUCCAUCUUAGCCCUAGUCACGGCUAGCUGUGAAAGGUCCGUGAGCCGCU
experiment.sample.molecule[0].organismHepatitis C virus
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeRNA
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw17.8
experiment.sample.molecule[0].total_mw35.6
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
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experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
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experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameSubdomain SL2' of hepatitis C virus RNA domain 3'X at higher ionic strength
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressValencia, Spain
experiment.contributor[0].affiliation[0].full_nameUniversidad Católica de Valencia
experiment.contributor[0].affiliation[0].webpagehttps://www.ucv.es/
experiment.contributor[0].contributor_nameJosé
experiment.contributor[0].contributor_surnameGallego
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2015-11-11
experiment.storage_temperature27.0
experiment.cell_temperature27.0
experiment.exposure_time1.0
experiment.number_of_frames30
experiment.wavelength0.0886
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pddf_softwareATSAS GNOM
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i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of Subdomain SL2' of hepatitis C virus RNA domain 3'X at higher ionic strength in 10mM Tris 0,1mM EDTA 2mM MgCl2, pH 7 were collected on the 12ID-B SAXS/WAXS camera on the storage ring Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.0886 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). Solute concentrations ranging between 0.5 and 2 mg/ml were measured at 27°C. 30 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for RNA concentration. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
tags[]
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experimental_mw40.1
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guinier_i0_mw_errornull
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porod_mw_errornull
pddf_i00.1406
pddf_i0_error0.0
guinier_i00.14
guinier_i0_error0.0004
pddf_rg4.36
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pddf_dmax16.9
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guinier_point_last15
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pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDBR7_datloglog_img.png
symmetrynull
last_modified2023-03-02T16:54:16.648202+01:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDBR7
statussuccess
started_at2026-08-11T16:43:57.244326+00:00
finished_at2026-08-11T16:44:04.792131+00:00
source_last_modified2023-03-02T16:54:16.648202+01:00
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View the complete summary.json source
{
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  "status": "Published",
  "type_of_curve": "Extrapolated to infinite dilution",
  "angular_unit": "1/A",
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    "title": "Three-dimensional structure of the 3'X-tail of hepatitis C virus RNA in monomeric and dimeric states.",
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      "author_list": "Cantero-Camacho Á, Fan L, Wang YX, Gallego J",
      "journal": "RNA",
      "doi": "10.1261/rna.060632.117",
      "pmid": "28630140",
      "published_date": "2017 Sep"
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  "experiment_description": "Synchrotron SAXS data from solutions of Subdomain SL2' of hepatitis C virus RNA domain 3'X at higher ionic strength in 10mM Tris 0,1mM EDTA 2mM MgCl2, pH 7 were collected on the 12ID-B SAXS/WAXS camera on the storage ring Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.0886 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). Solute concentrations ranging between 0.5 and 2 mg/ml were measured at 27°C. 30 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for RNA concentration. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.",
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  "last_modified": "2023-03-02T16:54:16.648202+01:00",
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View the complete manifest.json source
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