SASDBT9

Full-length human βB2-crystallin

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2023-03-02T16:54:16.648202+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Full-length human βB2-crystallin

浓度— – 2.2 mg/ml缓冲液 / pH25 mM NaPi, 5 mM DTT, 1 mM EDTA, / 6.5
实验温度— 设备 / 束线Advanced Photon Source (APS), Argonne National Laboratory / 12-ID-B SAXS/WAXS
波长0.08856 nm曝光0.6 s × 30

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Beta-crystallin B2
查看序列
>|FULL-LENGTHHUMANΒB2-CRYSTALLINASDHQTQAGKPQSLNPKIIIFEQENFQGHSHELNGPCPNLKETGVEKAGSVLVQAGPWVGYEQANCKGEQFVFEKGEYPRWDSWTSSRRTDSLSSLRPIKVDSQEHKIILYENPNFTGKKMEIIDDDVPSFHAHGYQEKVSSVRVQSGTWVGYQYPGYRGLQYLLEKGDYKDSSDFGAPHPQVQSVRRIRDMQWHQRGAFHPSN
proteinHomo sapiensP43320—–—dimer分子数 223.248 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1172[3]0.038098–0.449949 1/A含误差列缺失 00sasbdb/entries/t9/sasdbt9/source/SASDBT9.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)8.1—Å
i0Guinier0.0740.001—
i0P(r)0.07490.0007—
mwExperimental42.1—kDa
mwPorod42.1—kDa
porod_volumePorod67.43—ų
rgGuinier2.2380.04Å
rgP(r)2.2580.029Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称25 mM NaPi, 5 mM DTT, 1 mM EDTA,缓冲液浓度—
pH6.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2015-03-20储存 / 测量温度— / —
曝光时间0.6帧数30
波长0.08856样品-探测器距离2.0
光源X-ray synchrotron探测器Pilatus 2M
机构 / 束线Advanced Photon Source (APS), Argonne National Laboratory / 12-ID-B SAXS/WAXS · Lemont, IL, USA
q 范围0.381 – 4.499样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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pddfsasbdb/entries/t9/sasdbt9/source/SASDBT9.outdownloaded1986505b90f63139e687b61b67c37ded0367e74d54e4a68c69622a69966cb94390e6e下载查看原文件源站
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summarysasbdb/entries/t9/sasdbt9/source/summary.jsondownloaded7345bb9beefce91f3961867855f6423315825904906edae9b43cfba60018a42f7169下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDBT9
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleHuman βB2-Crystallin Forms a Face-en-Face Dimer in Solution: An Integrated NMR and SAXS Study.
project.publication.titleHuman βB2-Crystallin Forms a Face-en-Face Dimer in Solution: An Integrated NMR and SAXS Study.
project.publication.author_listXi Z, Whitley MJ, Gronenborn AM
project.publication.journalStructure
project.publication.doi10.1016/j.str.2017.02.001
project.publication.pmid28238532
project.publication.published_date2017 Mar 7
project.statusreleased
project.submitted_date2017-01-17
project.released_date2017-03-21
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experiment.instrument.detector.resolutionnull
experiment.instrument.nameAdvanced Photon Source (APS), Argonne National Laboratory
experiment.instrument.cityLemont, IL
experiment.instrument.countryUSA
experiment.instrument.beamline_name12-ID-B SAXS/WAXS
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameBeta-crystallin B2
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequence>|full-length human βB2-crystallin ASDHQTQAGKPQSLNPKIIIFEQENFQGHSHELNGPCPNLKETGVEKAGSVLVQAGPWVGYEQANCKGEQFVFEKGEYPRWDSWTSSRRTDSLSSLRPIKVDSQEHKIILYENPNFTGKKMEIIDDDVPSFHAHGYQEKVSSVRVQSGTWVGYQYPGYRGLQYLLEKGDYKDSSDFGAPHPQVQSVRRIRDMQWHQRGAFHPSN
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP43320
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.molecule[0].mw23.248
experiment.sample.molecule[0].total_mw46.496
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name25 mM NaPi, 5 mM DTT, 1 mM EDTA,
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph6.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameFull-length human βB2-crystallin
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressPittsburgh, PA USA
experiment.contributor[0].affiliation[0].full_nameUniversity of Pittsburgh
experiment.contributor[0].affiliation[0].webpagehttp://www.pitt.edu/
experiment.contributor[0].contributor_nameZhaoyong
experiment.contributor[0].contributor_surnameXi
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitmg/ml
experiment.date2015-03-20
experiment.storage_temperaturenull
experiment.cell_temperaturenull
experiment.exposure_time0.6
experiment.number_of_frames30
experiment.wavelength0.08856
experiment.sample_detector_distance2.0
experiment.concentration_minnull
experiment.concentration_max2.2
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.381
experiment.s_max4.499
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].bead_radius2.25
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fits[0].p_value0.43
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fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionATSAS 2.6.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of full-length human βB2-crystallin in 25 mM sodium phosphate, 5 mM DTT, 1 mM EDTA, pH 6.5, were collected on the 12ID-B SAXS/WAXS beam line at the Advanced Photon Source (APS, Argonne, IL, USA) using a Pilatus 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.08856 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Data were acquired over 30 successive 0.600 second frames from a sample at 2.2 mg/ml. The data were normalized to the intensity of the transmitted beam and radially averaged. The scattering of the radially-averaged solvent-blank was subtracted to produce the scattering profile displayed in this entry.
tags[]
intensity_unitnull
experimental_mw42.1
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw42.1
porod_mw_errornull
pddf_i00.0749
pddf_i0_error0.0007
guinier_i00.074
guinier_i0_error0.001
pddf_rg2.258
pddf_rg_error0.029
guinier_rg2.238
guinier_rg_error0.04
pddf_dmax8.1
pddf_dmax_errornull
porod_volume67.43
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last16
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDBT9_datloglog_img.png
symmetrynull
last_modified2023-03-02T16:54:16.648202+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDBT9
statussuccess
started_at2026-08-11T16:21:26.827802+00:00
finished_at2026-08-11T16:21:34.783430+00:00
source_last_modified2023-03-02T16:54:16.648202+01:00
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查看完整 summary.json 原文
{
  "code": "SASDBT9",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Human βB2-Crystallin Forms a Face-en-Face Dimer in Solution: An Integrated NMR and SAXS Study.",
    "publication": {
      "title": "Human βB2-Crystallin Forms a Face-en-Face Dimer in Solution: An Integrated NMR and SAXS Study.",
      "author_list": "Xi Z, Whitley MJ, Gronenborn AM",
      "journal": "Structure",
      "doi": "10.1016/j.str.2017.02.001",
      "pmid": "28238532",
      "published_date": "2017 Mar 7"
    },
    "status": "released",
    "submitted_date": "2017-01-17",
    "released_date": "2017-03-21"
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            "address": "Pittsburgh, PA USA",
            "full_name": "University of Pittsburgh",
            "webpage": "http://www.pitt.edu/"
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}
查看完整 manifest.json 原文
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