5. Experiment Description and Publication Experiment & Publication
Original Experiment Description
Synchrotron SAXS data from solutions of the DNA binding domain (DBD) of chromo domain-containing protein 1 (Chd1: 1009-1274) in 50mM HEPES, 150mM NaCl, pH 7.5 were collected on the X33 beam line at the DORIS III storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 5 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsin θ/λ and 2θ is the scattering angle). Solute concentrations ranging between 1.2 and 9.9 mg/ml were measured at 10°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Related Publication
Title
Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes.
Authors
Sundaramoorthy R, Hughes AL, Singh V, Wiechens N, Ryan DP, El-Mkami H, Petoukhov M, Svergun DI, Treutlein B, Quack S, Fischer M, Michaelis J, Böttcher B, Norman DG, Owen-Hughes T
DOI / PMID
10.7554/eLife.22510 / 28332978
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6. Complete Source Record Complete Source Record
The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.
Every source field is expanded here, including empty values, list members and fields already shown above.
summary.json：156 field values
Field path
Raw value
code
SASDBU7
status
Published
type_of_curve
Merged
angular_unit
1/nm
project.title
Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes.
project.publication.title
Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes.
project.publication.author_list
Sundaramoorthy R, Hughes AL, Singh V, Wiechens N, Ryan DP, El-Mkami H, Petoukhov M, Svergun DI, Treutlein B, Quack S, Fischer M, Michaelis J, Böttcher B, Norman DG, Owen-Hughes T
The DNA binding module of Saccharomyces cerevisiae Chd1 protein.
experiment_description
Synchrotron SAXS data from solutions of the DNA binding domain (DBD) of chromo domain-containing protein 1 (Chd1: 1009-1274) in 50mM HEPES, 150mM NaCl, pH 7.5 were collected on the X33 beam line at the DORIS III storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 5 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsin θ/λ and 2θ is the scattering angle). Solute concentrations ranging between 1.2 and 9.9 mg/ml were measured at 10°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
