查看完整 summary.json 原文
{
"code": "SASDBU8",
"status": "Published",
"type_of_curve": "Merged",
"angular_unit": "1/A",
"project": {
"title": "Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.",
"publication": {
"title": "Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.",
"author_list": "Shah N, Beckham SA, Wilce JA, Wilce MCJ",
"journal": "Nucleic Acids Res",
"doi": "10.1093/nar/gkx1307",
"pmid": "29346611",
"published_date": "2018 Apr 6"
},
"status": "released",
"submitted_date": "2016-12-11",
"released_date": "2018-01-22"
},
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"experiment": {
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"name": "Pilatus 1M",
"resolution": 172.0
},
"name": "Australian Synchrotron",
"city": "Melbourne",
"country": "Australia",
"beamline_name": "SAXS/WAXS",
"beam_geometry": "Point",
"type_of_source": "X-ray synchrotron",
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},
"sample": {
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{
"long_name": "5´ppp 8mer hairpin dsRNA",
"short_name": "8bp",
"sequence": "GGCGCGGCUUCGGCCGCGCC",
"organism": null,
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"oligomerization": "dimer",
"molecular_type": "RNA",
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},
{
"long_name": "Probable ATP-dependent RNA helicase DDX58 (without CARDs)",
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"sequence": "MHHHHHHAAASPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIATVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICKDLENLSQIQNREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYNDALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESVSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGNPKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPKPVPDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFDPAEMSK",
"organism": "Homo sapiens",
"uniprot_code": "O95786",
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"uniprot_range_last": 925,
"oligomerization": "dimer",
"molecular_type": "protein",
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"mw": 79.77,
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"deuteration": null,
"molecule_source": "biological",
"molecule_description": null
}
],
"buffer": {
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"concentration_unit": null,
"comment": null,
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"ph": 7.4,
"deuteration": null
},
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"name": "Probable ATP-dependent RNA helicase DDX58 (Delta-CARDs) plus 8mer hairpin dsRNA/AMP-PNP (SEC-peak2)",
"ext_coefficient": null,
"contrast": null,
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"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": "Melbourne, Australia",
"full_name": "Monash University",
"webpage": "http://www.monash.edu/"
}
],
"contributor_name": "Neelam",
"contributor_surname": "Shah",
"orcid": null
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2016-04-27",
"storage_temperature": 4.0,
"cell_temperature": 15.0,
"exposure_time": 5.0,
"number_of_frames": 15,
"wavelength": 0.10332,
"sample_detector_distance": 1.6,
"concentration_min": null,
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"flow_rate": null,
"s_min": 0.15,
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},
"fits": [],
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"pddf_software": "ATSAS GNOM",
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"i0_calibration_standard": null,
"description": null,
"experiment_description": "Synchrotron SAXS data from solutions of Probable ATP-dependent RNA helicase DDX58 (Delta-CARDs) plus 8mer hairpin dsRNA/AMP-PNP (SEC-peak2) in 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 0.5 mM AMP-PNP, pH 7.4 were collected using size exclusion chromatography (SEC-SAXS) on the SAXS/WAXS camera on the storage ring Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 1.6 m and at a wavelength of λ = 0.10332 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). Data frames (15 x 5 s exposures) were collected through the SEC elution peak at 32.8 min (GE Healthcare Life Sciences Superdex 200 10/300; 0.4 ml/min; 50 μl injection at 8 mg/ml). The data were normalized to the intensity of the transmitted beam and radially averaged; the radially averaged scattering of the solvent-blank was subtracted and the different curves were scaled to yield the final composite scattering curve. The SAXS data were collected at 15 °C.",
"tags": [],
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"experimental_mw": 173.1,
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"guinier_i0_mw": null,
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"pddf_i0": 0.0112,
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"pddf_dmax": 15.4,
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"porod_volume": 225.79,
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"estimated_volume": null,
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"guinier_point_first": 18,
"guinier_point_last": 68,
"pddf_point_first": null,
"pddf_point_last": null,
"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDBU8_datloglog_img.png",
"symmetry": null,
"last_modified": "2019-12-06T13:07:15.624532+01:00",
"bragg_peak": []
}查看完整 manifest.json 原文
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