The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.
Every source field is expanded here, including empty values, list members and fields already shown above.
View the complete summary.json source
{
"code": "SASDBV8",
"status": "Published",
"type_of_curve": "Merged",
"angular_unit": "1/A",
"project": {
"title": "Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.",
"publication": {
"title": "Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.",
"author_list": "Shah N, Beckham SA, Wilce JA, Wilce MCJ",
"journal": "Nucleic Acids Res",
"doi": "10.1093/nar/gkx1307",
"pmid": "29346611",
"published_date": "2018 Apr 6"
},
"status": "released",
"submitted_date": "2016-12-11",
"released_date": "2018-01-22"
},
"pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDBV8.out",
"intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDBV8.dat",
"intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBV8_dat_img.png",
"intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBV8_kratky_img.png",
"pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBV8_pofr_img.png",
"intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBV8_guinier_img.png",
"sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDBV8.sascif",
"experiment": {
"instrument": {
"detector": {
"type": "Dectris",
"name": "Pilatus 1M",
"resolution": 172.0
},
"name": "Australian Synchrotron",
"city": "Melbourne",
"country": "Australia",
"beamline_name": "SAXS/WAXS",
"beam_geometry": "Point",
"type_of_source": "X-ray synchrotron",
"point_source": true,
"line_collimation": null,
"sample_path_length": null,
"line_collimation_slitlength": null,
"line_collimation_integrationwidth": null,
"xray_energy": null,
"beam_profile_ah": null,
"beam_profile_al": null
},
"sample": {
"molecule": [
{
"long_name": "5´ppp 8mer hairpin dsRNA",
"short_name": "8bp",
"sequence": "GGCGCGGCUUCGGCCGCGCC",
"organism": null,
"uniprot_code": null,
"uniprot_range_first": 1,
"uniprot_range_last": 20,
"oligomerization": "dimer",
"molecular_type": "RNA",
"uniprot_sequence": null,
"mw": 6.484,
"total_mw": 12.968,
"number_molecules": 2,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": null
},
{
"long_name": "Probable ATP-dependent RNA helicase DDX58 (without CARDs)",
"short_name": "DC-RIG-I",
"sequence": "MHHHHHHAAASPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIATVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICKDLENLSQIQNREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYNDALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESVSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGNPKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPKPVPDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFDPAEMSK",
"organism": "Homo sapiens",
"uniprot_code": "O95786",
"uniprot_range_first": 239,
"uniprot_range_last": 925,
"oligomerization": "dimer",
"molecular_type": "protein",
"uniprot_sequence": "MTTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAEKNNKGPMEAATLFLKF\nLLELQEEGWFRGFLDALDHAGYSGLYEAIESWDFKKIEKLEEYRLLLKRLQPEFKTRIIP\nTDIISDLSECLINQECEEILQICSTKGMMAGAEKLVECLLRSDKENWPKTLKLALEKERN\nKFSELWIVEKGIKDVETEDLEDKMETSDIQIFYQEDPECQNLSENSCPPSEVSDTNLYSP\nFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFANQI\nPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQILVNNLKKGTI\nPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSGPLPQVIGLTASVGVGDAKNT\nDEALDYICKLCASLDASVIATVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMR\nDTESLAKRICKDLENLSQIQNREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFL\nYTSHLRKYNDALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESV\nSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGNPKLSFLKPGI\nLTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVADEGIDIAQCNLVILYEYVGN\nVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEAVFREKI\nLHIQTHEKFIRDSQEKPKPVPDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKE\nCFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATG\nVQTLYSKWKDFHFEKIPFDPAEMSK",
"mw": 79.77,
"total_mw": 159.54,
"number_molecules": 2,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": null
}
],
"buffer": {
"name": "25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 2mM ADP-AlFx",
"concentration_unit": null,
"comment": null,
"additive": null,
"concentration": null,
"pka": null,
"ph": 7.4,
"deuteration": null
},
"purity_method": null,
"name": "Probable ATP-dependent RNA helicase DDX58 (Delta-CARDs) plus 8mer hairpin dsRNA/ADP-AlFx (SEC-peak2)",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": "Melbourne, Australia",
"full_name": "Monash University",
"webpage": "http://www.monash.edu/"
}
],
"contributor_name": "Neelam",
"contributor_surname": "Shah",
"orcid": null
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2015-11-20",
"storage_temperature": 4.0,
"cell_temperature": 15.0,
"exposure_time": 2.0,
"number_of_frames": 13,
"wavelength": 0.10332,
"sample_detector_distance": 1.6,
"concentration_min": null,
"concentration_max": null,
"sample_volume": null,
"flow_rate": null,
"s_min": 0.107,
"s_max": 2.498,
"total_exposure_time": null,
"seccolumn": null
},
"fits": [
{
"models": [
{
"model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDBV8_fit1_model1_img.png",
"software": "SASREF",
"pdb_link": [],
"model_title": null,
"type_of_model": "atomic",
"software_version": "v6.0",
"model_data": "https://www.sasbdb.org/media/pdb_file/SASDBV8_fit1_model1.pdb",
"model_mw": 156.4,
"bead_radius": 1.9,
"log": null,
"symmetry": "p1",
"comment": "",
"user": 168
}
],
"fit_unit": "1/A",
"fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDBV8_fit1_fit_img.png",
"software": "SASREF",
"chi_square_value": 0.26,
"p_value": 0.0,
"fit_residual_plot": "SASDBV8_fit1_fitresiduals_img.png",
"fit_data": "https://www.sasbdb.org/media/fitting_files/SASDBV8_fit1.fit",
"fit_log": null,
"software_version": "v6.0",
"description": null
}
],
"estimated_volume_method": null,
"pddf_software": "ATSAS GNOM",
"pddf_software_version": "GNOM v4.5",
"i0_calibration_standard": null,
"description": null,
"experiment_description": "Synchrotron SAXS data from solutions of Probable ATP-dependent RNA helicase DDX58 (Delta-CARDs) plus 8mer hairpin dsRNA/ADP-AlFx (SEC-peak2) in 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 2mM ADP-AlFx, pH 7.4 were collected using size exclusion chromatography (SEC-SAXS) on the SAXS/WAXS camera on the storage ring Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 1.6 m and at a wavelength of λ = 0.10332 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). Data frames (13 x 2 s exposures) were collected through the SEC elution peak at 32.7 min (GE Healthcare Life Sciences Superdex 200 10/300; 0.4 ml/min; 100 μl injection at 8 mg/ml). The data were normalized to the intensity of the transmitted beam and radially averaged; the radially averaged scattering of the solvent-blank was subtracted and the different curves were scaled to yield the final composite scattering curve. The SAXS data were collected at 15 °C.",
"tags": [],
"intensity_unit": null,
"experimental_mw": 173.1,
"experimental_mw_error": null,
"guinier_i0_mw": null,
"guinier_i0_mw_error": null,
"porod_mw": 135.4,
"porod_mw_error": null,
"pddf_i0": null,
"pddf_i0_error": null,
"guinier_i0": 0.0205,
"guinier_i0_error": null,
"pddf_rg": 4.01,
"pddf_rg_error": null,
"guinier_rg": 3.92,
"guinier_rg_error": null,
"pddf_dmax": 16.0,
"pddf_dmax_error": null,
"porod_volume": 230.13,
"porod_volume_error": null,
"estimated_volume": null,
"estimated_volume_error": null,
"guinier_point_first": 7,
"guinier_point_last": 56,
"pddf_point_first": null,
"pddf_point_last": null,
"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDBV8_datloglog_img.png",
"symmetry": null,
"last_modified": "2019-12-06T13:07:15.624532+01:00",
"bragg_peak": []
}View the complete manifest.json source
{
"code": "SASDBV8",
"status": "success",
"started_at": "2026-08-11T16:01:30.823425+00:00",
"finished_at": "2026-08-11T16:01:40.576742+00:00",
"source_last_modified": "2019-12-06T13:07:15.624532+01:00",
"files": [
{
"status": "downloaded",
"url": "https://www.sasbdb.org/rest-api/entry/summary/SASDBV8/",
"path": "/data/saxsdb/sasbdb/entries/v8/sasdbv8/source/summary.json",
"size": 9626,
"sha256": "c647da18234f8e6ac4d1f2bfa16826e6836ef8c7ebb6dda065551d94775e0f9d"
},
{
"status": "downloaded",
"url": "https://www.sasbdb.org/media/intensities_files/SASDBV8.dat",
"path": "/data/saxsdb/sasbdb/entries/v8/sasdbv8/source/SASDBV8.dat",
"size": 24885,
"sha256": "d03fe03fae8c563328bdb2b1af51775ace6149e70405ce351e8dc49ead7bc26a",
"role": "curve"
},
{
"status": "downloaded",
"url": "https://www.sasbdb.org/media/p_of_R_files/SASDBV8.out",
"path": "/data/saxsdb/sasbdb/entries/v8/sasdbv8/source/SASDBV8.out",
"size": 41149,
"sha256": "8f7e1801e55e6667d2a62a5a7eee52679ce6d6037244ba81d19ae67ea11d2093",
"role": "pddf"
},
{
"status": "downloaded",
"url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDBV8.sascif",
"path": "/data/saxsdb/sasbdb/entries/v8/sasdbv8/source/SASDBV8.sascif",
"size": 1246678,
"sha256": "4ead63a3cd68529f5f0568ebb803a3012b0e7994d6c6d5076d3bed4aae54e960",
"role": "sascif"
},
{
"status": "downloaded",
"url": "https://www.sasbdb.org/media/zip_directories/SASDBV8.zip",
"path": "/data/saxsdb/sasbdb/entries/v8/sasdbv8/source/SASDBV8.zip",
"size": 210215,
"sha256": "f4815dccac1b7e4c33c9692f18b51cfff15cd9cf77a5fafb51133246ca1aa535",
"role": "full_entry_zip",
"zip_member_count": 4,
"zip_validation": "central_directory_readable"
}
]
}
