SASDBX2

Zebrafish Arpin in complex with the ankyrin repeat domains of human Tankyrase 2 (489-469)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2019-12-06T13:07:15.624532+01:00

Due to high concentration at the elution peak, the HPLC UV detector was saturated and the concentrations of the sample was consequently underestimated. In this case, the molecular mass is overestimated when assessing this parameter from I(0) and concentration.

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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Zebrafish Arpin in complex with the ankyrin repeat domains of human Tankyrase 2 (489-469)

浓度— – — 缓冲液 / pH50 mM HEPES 100mM NaCl 1mM TCEP / 7.5
实验温度15.0 设备 / 束线SOLEIL / SWING
波长0.1 nm曝光1.5 s × —

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Zebrafish arpin/human tankyrase 2 ankyrin repeat domain complex
查看序列
GSRIYDNTALLNKPVHNEKLSFTWDPIVHQSGHGVILEGTVVDFSRHAITDVKNRKERYNVLYIKPSRVHRRKYDSKGNEIEPNFSDTKKVNTGFLMSSFKVEAKGETDCLDERQLREIVNKEQLVKVTIKHCPREAFAFWISEAEMDKTELEPGQEVRLKTKGDGPFIFSSAKLDSGTVTKCNFAGDENAGASWTEKIMANKSNQENTGKSAAQGEGADDDEWDDGNSEADRQLLEAAKAGDVETVKKLCTVQSVNCRDIEGRQSTPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLDLVKDGDTDIQDLLRGDAAL
proteinDanio rerio / Homo sapiens——–—monomer分子数 143.018 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1559[3]0.152458–2.48064 1/nm含误差列缺失 00sasbdb/entries/x2/sasdbx2/source/SASDBX2.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)12.6—nm
i0Guinier0.072——
i0P(r)0.072——
mwExperimental38.4—kDa
mwPorod34.0—kDa
porod_volumePorod57.9—nm³
rgGuinier3.3—nm
rgP(r)3.35—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50 mM HEPES 100mM NaCl 1mM TCEP缓冲液浓度50.0 mM
pH7.5添加剂100mM NaCl, 1mM TCEP
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2014-12-04储存 / 测量温度15.0 / 15.0
曝光时间1.5帧数—
波长0.1样品-探测器距离1.82
光源X-ray synchrotron探测器AVIEX PCCD170170
机构 / 束线SOLEIL / SWING · Saint-Aubin, France
q 范围0.152 – 2.481样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:134 个字段值
字段路径原始值
codeSASDBX2
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleHybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope.
project.publication.titleHybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope.
project.publication.author_listFetics S, Thureau A, Campanacci V, Aumont-Nicaise M, Dang I, Gautreau A, Pérez J, Cherfils J
project.publication.journalStructure
project.publication.doi10.1016/j.str.2015.12.001
project.publication.pmid26774128
project.publication.published_date2016 Feb 2
project.statusreleased
project.submitted_date2015-12-03
project.released_date2016-07-03
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experiment.instrument.detector.resolutionnull
experiment.instrument.nameSOLEIL
experiment.instrument.citySaint-Aubin
experiment.instrument.countryFrance
experiment.instrument.beamline_nameSWING
experiment.instrument.beam_geometry
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experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
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experiment.instrument.xray_energynull
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experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameZebrafish arpin/human tankyrase 2 ankyrin repeat domain complex
experiment.sample.molecule[0].short_nameArpin/tankyrase
experiment.sample.molecule[0].sequenceGSRIYDNTALLNKPVHNEKLSFTWDPIVHQSGHGVILEGTVVDFSRHAITDVKNRKERYNVLYIKPSRVHRRKYDSKGNEIEPNFSDTKKVNTGFLMSSFKVEAKGETDCLDERQLREIVNKEQLVKVTIKHCPREAFAFWISEAEMDKTELEPGQEVRLKTKGDGPFIFSSAKLDSGTVTKCNFAGDENAGASWTEKIMANKSNQENTGKSAAQGEGADDDEWDD GNSEADRQLLEAAKAGDVETVKKLCTVQSVNCRDIEGRQSTPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLDLVKDGDTDIQDLLRGDAAL
experiment.sample.molecule[0].organismDanio rerio / Homo sapiens
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw43.018
experiment.sample.molecule[0].total_mw43.018
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateTrue
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name50 mM HEPES 100mM NaCl 1mM TCEP
experiment.sample.buffer.concentration_unitmM
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive100mM NaCl, 1mM TCEP
experiment.sample.buffer.concentration50.0
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameZebrafish Arpin in complex with the ankyrin repeat domains of human Tankyrase 2 (489-469)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameSoleil
experiment.contributor[0].affiliation[0].addressSaint-Aubin, France
experiment.contributor[0].affiliation[0].full_nameSoleil Synchrotron
experiment.contributor[0].affiliation[0].webpagehttp://www.synchrotron-soleil.fr/
experiment.contributor[0].contributor_nameAurélien
experiment.contributor[0].contributor_surnameThureau
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2014-12-04
experiment.storage_temperature15.0
experiment.cell_temperature15.0
experiment.exposure_time1.5
experiment.number_of_framesnull
experiment.wavelength0.1
experiment.sample_detector_distance1.82
experiment.concentration_minnull
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experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.152
experiment.s_max2.481
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionDue to high concentration at the elution peak, the HPLC UV detector was saturated and the concentrations of the sample was consequently underestimated. In this case, the molecular mass is overestimated when assessing this parameter from I(0) and concentration.
experiment_descriptionX-ray synchrotron radiation scattering data from solutions of zebrafish arpin bound to the ankyrin repeat domains of human Tankyrase-2 (amino acids 489-649) in 50mM HEPES, pH 7.5, 100mM NaCl, 1mM TCEP were collected at 15 C on the SWING Beamline, Synchrotron Soleil, Gif Sur Yvette, France using a PCCD170170 Aviex detector. These data are derived from size-exclusion chromatography-SAXS. Note: The original SAXS data where the momentum transfer s in inverse Angstroms (s = 4π sin θ/λ, where 2θ is the scattering angle) is included in the zip file associated with this entry. Refer to UniProt ID Q9H2K2 (amino acids 489-649) and Q1LWJ6 for additional sequence information.
tags[]
intensity_unitnull
experimental_mw38.4
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw34.0
porod_mw_errornull
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pddf_dmax_errornull
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porod_volume_errornull
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estimated_volume_errornull
guinier_point_first2
guinier_point_last77
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDBX2_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDBX2
statussuccess
started_at2026-08-11T13:48:19.628456+00:00
finished_at2026-08-11T13:48:27.007104+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
{
  "code": "SASDBX2",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope.",
    "publication": {
      "title": "Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope.",
      "author_list": "Fetics S, Thureau A, Campanacci V, Aumont-Nicaise M, Dang I, Gautreau A, Pérez J, Cherfils J",
      "journal": "Structure",
      "doi": "10.1016/j.str.2015.12.001",
      "pmid": "26774128",
      "published_date": "2016 Feb 2"
    },
    "status": "released",
    "submitted_date": "2015-12-03",
    "released_date": "2016-07-03"
  },
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          "organism": "Danio rerio / Homo sapiens",
          "uniprot_code": null,
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          "oligomerization": "monomer",
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          "number_molecules": 1,
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      },
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      "contrast": null,
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      "dry_vol": null,
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    },
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  "description": "Due to high concentration at the elution peak, the HPLC UV detector was saturated and the concentrations of the sample was consequently underestimated. In this case, the molecular mass is overestimated when assessing this parameter from I(0) and concentration.",
  "experiment_description": "X-ray synchrotron radiation scattering data from solutions of zebrafish arpin bound to the ankyrin repeat domains of human Tankyrase-2 (amino acids 489-649) in 50mM HEPES, pH 7.5, 100mM NaCl, 1mM TCEP were collected at 15 C on the SWING Beamline, Synchrotron Soleil, Gif Sur Yvette, France using a PCCD170170 Aviex detector. These data are derived from size-exclusion chromatography-SAXS. Note: The original SAXS data where the momentum transfer s in inverse Angstroms (s = 4π sin θ/λ, where 2θ is the scattering angle) is included in the zip file associated with this entry. Refer to UniProt ID Q9H2K2 (amino acids 489-649) and Q1LWJ6 for additional sequence information.\r\n",
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  "intensities_log_log_plot": "SASDBX2_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
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}
查看完整 manifest.json 原文
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