SASDBY3

Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen voltage) module (light state-TBE)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2019-12-06T13:07:15.624532+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen voltage) module (light state-TBE)

浓度— – 1.25 mg/ml缓冲液 / pH50 mM Tris 50 mM boric acid 1 mM EDTA / 8.0
实验温度4.0 设备 / 束线ESRF / BM29
波长0.93 nm曝光20.0 s × 10

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Aureochrome 1a bZIP-LOV module
查看序列
MGSSHHHHHHSSGLVPRGSHMDRKMSEQQKVERRERNREHAKRSRIRKKFLLESLQQSVSLLKEENEKLKTSIRSHLGEEKADTLIDSANNNKTDVDGLLASSQGIANKVLDDPDFSFIKALQTAQQNFVVTDPSLPDNPIVYASQGFLNLTGYSLDQILGRNCRFLQGPETDPKAVERIRKAIEQGNDMSVCLLNYRVDGTTFWNQFFIAALRDAGGNVTNFVGVQCKVSDQYAATVTKQQEEEEEAAANDDED
proteinPhaeodactylum tricornutum—145–378dimer分子数 228.655 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11043[3]0.03637825–4.974514 1/nm含误差列缺失 028sasbdb/entries/y3/sasdby3/source/SASDBY3.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)12.64—nm
i0Guinier57.85——
i0P(r)57.85——
mwExperimental57.5—kDa
mwPorod57.5—kDa
porod_volumePorod115.0—nm³
rgGuinier3.41—nm
rgP(r)3.45—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50 mM Tris 50 mM boric acid 1 mM EDTA缓冲液浓度50.0 mM
pH8.0添加剂50 mM boric acid, 1 mM EDTA
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2014-11-06储存 / 测量温度4.0 / 4.0
曝光时间20.0帧数10
波长0.93样品-探测器距离2.43
光源X-ray synchrotron探测器Pilatus 1M
机构 / 束线ESRF / BM29 · Grenoble, France
q 范围0.036 – 4.975样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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pddf:来源记录
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sascif:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDBY3
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
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project.publication.titleAllosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes.
project.publication.author_listBanerjee A, Herman E, Serif M, Maestre-Reyna M, Hepp S, Pokorny R, Kroth PG, Essen LO, Kottke T
project.publication.journalNucleic Acids Res
project.publication.doi10.1093/nar/gkw420
project.publication.pmid27179025
project.publication.published_date2016 Jul 8
project.statusreleased
project.submitted_date2016-02-14
project.released_date2016-09-19
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experiment.instrument.countryFrance
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experiment.sample.molecule[0].short_namePtAUREO1a bZIP-LOV
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experiment.sample.molecule[0].organismPhaeodactylum tricornutum
experiment.sample.molecule[0].uniprot_codenull
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experiment.sample.molecule[0].uniprot_range_last378
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experiment.sample.purity_methodnull
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experiment.sample.contrastnull
experiment.sample.specific_volnull
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experiment.contributor[0].affiliation[0].full_namePhilipps-Universität Marburg
experiment.contributor[0].affiliation[0].webpage
experiment.contributor[0].contributor_nameAnkan
experiment.contributor[0].contributor_surnameBanerjee
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experiment.concentration_unitmg/ml
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experiment.cell_temperature4.0
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pddf_softwareATSAS GNOM
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i0_calibration_standardnull
descriptionnull
experiment_descriptionX-ray synchrotron radiation scattering data from solution of PtAUREO1a bZIP-LOV module in 0.5X TBE (50 mM Tris pH 8, 50 mM boric acid, 1 mM EDTA) buffer was collected on the BM29 camera on the storage ring ESRF (Grenoble, France) using a 2D Photon counting Pilatus 1M-W pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). Ten successive 20 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. Solution scattering data of bZIP-LOV module in the light state was performed at 1.25mg/mL concentration at 4 degrees centigrade. The modelling analysis was performed using the ATSAS package. The displayed model is an averaged and volume corrected dummy atom representation (DAMFILT). For further sequence information refer to the Joint Genome Institute (JGI) entry JGI-49116.
tags[]
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intensities_log_log_plotSASDBY3_datloglog_img.png
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last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
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字段路径原始值
codeSASDBY3
statussuccess
started_at2026-08-11T14:10:46.109593+00:00
finished_at2026-08-11T14:10:54.842670+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
{
  "code": "SASDBY3",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes.",
    "publication": {
      "title": "Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes.",
      "author_list": "Banerjee A, Herman E, Serif M, Maestre-Reyna M, Hepp S, Pokorny R, Kroth PG, Essen LO, Kottke T",
      "journal": "Nucleic Acids Res",
      "doi": "10.1093/nar/gkw420",
      "pmid": "27179025",
      "published_date": "2016 Jul 8"
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}
查看完整 manifest.json 原文
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