查看完整 summary.json 原文
{
"code": "SASDC28",
"status": "Published",
"type_of_curve": "Other",
"angular_unit": "1/A",
"project": {
"title": "The atypical thiol-disulfide exchange protein α-DsbA2 from Wolbachia pipientis is a homotrimeric disulfide isomerase.",
"publication": {
"title": "The atypical thiol-disulfide exchange protein α-DsbA2 from Wolbachia pipientis is a homotrimeric disulfide isomerase.",
"author_list": "Walden PM, Whitten AE, Premkumar L, Halili MA, Heras B, King GJ, Martin JL",
"journal": "Acta Crystallogr D Struct Biol",
"doi": "10.1107/S2059798318018442",
"pmid": "30950399",
"published_date": "2019 Mar 1"
},
"status": "released",
"submitted_date": "2017-10-03",
"released_date": "2019-03-29"
},
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"name": "Pilatus 1M",
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},
"name": "Australian Synchrotron",
"city": "Melbourne",
"country": "Australia",
"beamline_name": "SAXS/WAXS",
"beam_geometry": "Point",
"type_of_source": "X-ray synchrotron",
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{
"long_name": "DsbA-like disulfide oxidoreductase (thiol-disulfide exchange protein)",
"short_name": "α-DsbA2",
"sequence": "SNAMSLPIINNWLSNRSQMLNDDYIGEKLDNYISRNFDKIVKTLKEESIKSSYAARDNV\r\nTKSKISQYKDQIFDLTYPYSGNENSSVIAVGFLDYSCGHCKAIKNDIKQLINDGKIKYIFRDAPILGNAS\r\nLKAAKSALAVYFLNKEKYFDFHHAALSHKGEFSDESILDIVKNIGIDEDDFNDSIKDNADKIEQMINNSR\r\nLLVRDLGVGGTPFLIIGDSLFVGATDLNVLRKKVDELSHKQG",
"organism": "Wolbachia endosymbiont of Drosophila melanogaster",
"uniprot_code": "Q73FL6",
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"oligomerization": "trimer",
"molecular_type": "protein",
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}
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"name": "Mature α-DsbA2 protein",
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"contributor": [
{
"affiliation": [
{
"short_name": "ANSTO",
"address": "Kirrawee DC, NSW 2232, Australia",
"full_name": "Australian Nuclear Science and Technology Organisation",
"webpage": ""
}
],
"contributor_name": "Andrew",
"contributor_surname": "Whitten",
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"date": "2014-03-29",
"storage_temperature": 4.0,
"cell_temperature": 20.0,
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"comment": "Filtered model from Damaver",
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}
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"experiment_description": "X-ray synchrotron radiation scattering data from the mature version of α-DsbA2 from Wolbachia pipientis in 25 mM TRIS 150mM NaCl pH 7.5 were collected on the SAXS/WAXS beam line of the Australian Synchrotron (Melbourne, Australia) in a SEC-SAXS configuration using a 2D Photon counting Pilatus 1M-W pixel detector (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle; λ=0.1127 nm). Approximately 100 uL of 19.8 mg/ml protein solution were injected onto a WTC-030S5 column at a flow rate of 0.5 ml/min. Five hundred successive 5 second frames were collected during the SEC-SAXS run. The peak of the elution profile occured at ~18 mins after the start of the run, and the protein scattering was taken as the average of 8 frames collected 35-75 seconds after the peak eluted (the radius of gyration dropped to ~27.6 Ang at the peak concentration of ~2.2 mg/ml, so data was averaged at a region where the concentration was lower and concentration dependent effects were not apparent). The concentration range corresponding to the averaged frames was ~0.35 - 1.15 mg/ml, and the average concentration was taken as ~0.75 mg/ml. The buffer was taken as the sum of 100 frames collected between 3-9 mins after the start of the run. The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering of the solvent-blank was subtracted. The models and corresponding fits include those derived from dummy-atom modelling using DAMMIN (top) and rigid-body modelling using CORAL (bottom).",
"tags": [],
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"symmetry": null,
"last_modified": "2021-04-10T09:53:07.171187+02:00",
"bragg_peak": []
}查看完整 manifest.json 原文
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