SASDC34

Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) R51E mutant 7.0 mg/mL

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2026-05-18T10:06:35.099964+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) R51E mutant 7.0 mg/mL

浓度— – 7.0 缓冲液 / pH50 mM Tris (pH 7.8), 50 mM NaCl, 0.5 mM Tris(2-carboxyethyl)phosphine, and 5% (v/v) glycerol / 7.8
实验温度— 设备 / 束线Advanced Light Source (ALS) / 12.3.1 (SIBYLS)
波长— nm曝光— s × —

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Proline dehydrogenase
查看序列
GHMPNIPPPFTAPYAPDDAEIAARLLPASHLSPPQEARIHRTATRLIEAIRKEDDRLGGVEDMLREFALSTKEGLALMVLAEALLRVPDARTADQFIEDKLGEGDFIHHETKSTAFLVNASAWALGLSARVIQPGETPDGTIGRLVKRLGAPAVRTATRQAMRLMGNHFVLGETIEQALERGKPRSGQKTRYSFDMLGEGARTAADARRYFDAYASAIETIGKAAGNHALPDRPGISVKLSALHPRFEAISRARVMVELVPQLLDLAQRAKAHDLNFTVDAEEADRLELSLDVIAATLADPSLKGWDGFGLAIQAYQKRASAVIDYVDALARAHDRKLMVRLVKGAYWDTEIKRAQERGLDGYPVFTRKAMTDLNYVACASKLLALRPRIFPQFATHNALTVATVLEMAEGSSGFEFQRLHGMGEALYEQLAKDHADIAYRTYAPVGSHRDLLAYLVRRLLENGANSSFVAQAADYRVPVPALLQRPADAIVRPQAAAHPRIPLPCDLFAPERRNSRGVEFGARTALDQLLTDVKAETGDLKPIADATPDQAHAAVAAARAGFAGWSRTPAGIRAAALEQAAHLLESRSAHFIALLQREGGKTLDDALSELREAADFCRYYAAQGRKLFGSETAMPGPTGESNALTMRGRGVFVAISPWNFPLAIFLGQVTAALMAGNSVVAKPAEQTPRIAREAVALLHEAGIPKSALYLVTGDGRIGAALTAHPDIAGVVFTGSTEVARSINRALAAKDGPIVPLIAETGGINAMIADATALPEQVADDVVTSAFRSAGQRCSALRLLFVQEDVADRMIEMVAGAARELKIGDPSDVATHVGPVIDVEAKQRLDAHIARMKTEARLHFAGPAPEGCFVAPHIFELTEAGQLTEEVFGPILHVVRYRPENLERVLRAIERTGYGLTLGVHSRIDDSIEAIIDRVQVGNIYVNRNMIGAVVGVQPFGGNGLSGTGPKAGGPHYLARFATEQTVTINTAAAGGNAALLAGEE
proteinBradyrhizobium diazoefficiensQ89E26—–—dimer分子数 2107.531 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1513[3]0.013075–0.324398 1/A含误差列缺失 00sasbdb/entries/34/sasdc34/source/SASDC34.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)14.6—Å
i0Guinier355.793.34—
i0P(r)357.00.7—
mwExperimental216.0—kDa
porod_volumePorod289.0—ų
rgGuinier4.50.04Å
rgP(r)4.590.01Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50 mM Tris (pH 7.8), 50 mM NaCl, 0.5 mM Tris(2-carboxyethyl)phosphine, and 5% (v/v) glycerol缓冲液浓度—
pH7.8添加剂—
缓冲液说明BjPutA buffer
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2016-12-16储存 / 测量温度— / —
曝光时间—帧数—
波长—样品-探测器距离—
光源X-ray synchrotron探测器Pilatus3 X 2M
机构 / 束线Advanced Light Source (ALS) / 12.3.1 (SIBYLS) · Berkeley, CA, USA
q 范围0.131 – 3.244样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
curvesasbdb/entries/34/sasdc34/source/SASDC34.datdownloaded23085eb35039fd864b04cfb96a5c02905fd558e3b54a55be5276bb27853a0c4251fb3下载查看原文件源站
full_entry_zipsasbdb/entries/34/sasdc34/source/SASDC34.zipdownloaded69919acece0d67c73f70d4103f17e74fa3e7b435216f09a9dfd5beffa7f1c7f59a98a下载查看原文件源站
pddfsasbdb/entries/34/sasdc34/source/SASDC34.outdownloaded4786771104dea5716f8d2e91462767e855a9e7904fb5cd4b90bc29db6d2c7b7acdf0f下载查看原文件源站
sascifsasbdb/entries/34/sasdc34/source/SASDC34.sascifdownloaded334693a2d6fbf5a4d4c6d03b782ecc119fa789c0e8bcc594c8c0374df32d24e8554401下载查看原文件源站
summarysasbdb/entries/34/sasdc34/source/summary.jsondownloaded9547f8e48a98c54a9bc26a661202b7b476819610db75a24bfbee92538b4cc1f811b6下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(5 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:169 个字段值
字段路径原始值
codeSASDC34
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleBiophysical investigation of type A PutAs reveals a conserved core oligomeric structure.
project.publication.titleBiophysical investigation of type A PutAs reveals a conserved core oligomeric structure.
project.publication.author_listKorasick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ
project.publication.journalFEBS J
project.publication.doi10.1111/febs.14165
project.publication.pmid28710792
project.publication.published_date2017 Sep
project.statusreleased
project.submitted_date2017-05-19
project.released_date2017-07-17
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 X 2M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameAdvanced Light Source (ALS)
experiment.instrument.cityBerkeley, CA
experiment.instrument.countryUSA
experiment.instrument.beamline_name12.3.1 (SIBYLS)
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameProline dehydrogenase
experiment.sample.molecule[0].short_nameBjPutA R51E
experiment.sample.molecule[0].sequenceGHMPNIPPPFTAPYAPDDAEIAARLLPASHLSPPQEARIHRTATRLIEAIRKEDDRLGGVEDMLREFALSTKEGLALMVL AEALLRVPDARTADQFIEDKLGEGDFIHHETKSTAFLVNASAWALGLSARVIQPGETPDGTIGRLVKRLGAPAVRTATRQ AMRLMGNHFVLGETIEQALERGKPRSGQKTRYSFDMLGEGARTAADARRYFDAYASAIETIGKAAGNHALPDRPGISVKL SALHPRFEAISRARVMVELVPQLLDLAQRAKAHDLNFTVDAEEADRLELSLDVIAATLADPSLKGWDGFGLAIQAYQKRA SAVIDYVDALARAHDRKLMVRLVKGAYWDTEIKRAQERGLDGYPVFTRKAMTDLNYVACASKLLALRPRIFPQFATHNAL TVATVLEMAEGSSGFEFQRLHGMGEALYEQLAKDHADIAYRTYAPVGSHRDLLAYLVRRLLENGANSSFVAQAADYRVPV PALLQRPADAIVRPQAAAHPRIPLPCDLFAPERRNSRGVEFGARTALDQLLTDVKAETGDLKPIADATPDQAHAAVAAAR AGFAGWSRTPAGIRAAALEQAAHLLESRSAHFIALLQREGGKTLDDALSELREAADFCRYYAAQGRKLFGSETAMPGPTG ESNALTMRGRGVFVAISPWNFPLAIFLGQVTAALMAGNSVVAKPAEQTPRIAREAVALLHEAGIPKSALYLVTGDGRIGA ALTAHPDIAGVVFTGSTEVARSINRALAAKDGPIVPLIAETGGINAMIADATALPEQVADDVVTSAFRSAGQRCSALRLL FVQEDVADRMIEMVAGAARELKIGDPSDVATHVGPVIDVEAKQRLDAHIARMKTEARLHFAGPAPEGCFVAPHIFELTEA GQLTEEVFGPILHVVRYRPENLERVLRAIERTGYGLTLGVHSRIDDSIEAIIDRVQVGNIYVNRNMIGAVVGVQPFGGNG LSGTGPKAGGPHYLARFATEQTVTINTAAAGGNAALLAGEE
experiment.sample.molecule[0].organismBradyrhizobium diazoefficiens
experiment.sample.molecule[0].uniprot_codeQ89E26
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMPNIPPPFTAPYAPDDAEIAARLLPASHLSPPQEARIHRTATRLIEAIRKRDDRLGGVED MLREFALSTKEGLALMVLAEALLRVPDARTADQFIEDKLGEGDFIHHETKSTAFLVNASA WALGLSARVIQPGETPDGTIGRLVKRLGAPAVRTATRQAMRLMGNHFVLGETIEQALERG KPRSGQKTRYSFDMLGEGARTAADARRYFDAYASAIETIGKAAGNHALPDRPGISVKLSA LHPRFEAISRARVMVELVPQLLDLAQRAKAHDLNFTVDAEEADRLELSLDVIAATLADPS LKGWDGFGLAIQAYQKRASAVIDYVDALARAHDRKLMVRLVKGAYWDTEIKRAQERGLDG YPVFTRKAMTDLNYVACASKLLALRPRIFPQFATHNALTVATVLEMAEGSSGFEFQRLHG MGEALYEQLAKDHADIAYRTYAPVGSHRDLLAYLVRRLLENGANSSFVAQAADYRVPVPA LLQRPADAIVRPQAAAHPRIPLPCDLFAPERRNSRGVEFGARTALDQLLTDVKAETGDLK PIADATPDQAHAAVAAARAGFAGWSRTPAGIRAAALEQAAHLLESRSAHFIALLQREGGK TLDDALSELREAADFCRYYAAQGRKLFGSETAMPGPTGESNALTMRGRGVFVAISPWNFP LAIFLGQVTAALMAGNSVVAKPAEQTPRIAREAVALLHEAGIPKSALYLVTGDGRIGAAL TAHPDIAGVVFTGSTEVARSINRALAAKDGPIVPLIAETGGINAMIADATALPEQVADDV VTSAFRSAGQRCSALRLLFVQEDVADRMIEMVAGAARELKIGDPSDVATHVGPVIDVEAK QRLDAHIARMKTEARLHFAGPAPEGCFVAPHIFELTEAGQLTEEVFGPILHVVRYRPENL ERVLRAIERTGYGLTLGVHSRIDDSIEAIIDRVQVGNIYVNRNMIGAVVGVQPFGGNGLS GTGPKAGGPHYLARFATEQTVTINTAAAGGNAALLAGEE
experiment.sample.molecule[0].mw107.531
experiment.sample.molecule[0].total_mw215.062
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name50 mM Tris (pH 7.8), 50 mM NaCl, 0.5 mM Tris(2-carboxyethyl)phosphine, and 5% (v/v) glycerol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentBjPutA buffer
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.8
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameProline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) R51E mutant 7.0 mg/mL
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameMizzou
experiment.contributor[0].affiliation[0].addressColumbia, MO, USA
experiment.contributor[0].affiliation[0].full_nameUniversity of Missouri-Columbia
experiment.contributor[0].affiliation[0].webpagehttp://missouri.edu/
experiment.contributor[0].contributor_nameJohn
experiment.contributor[0].contributor_surnameTanner
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2016-12-16
experiment.storage_temperaturenull
experiment.cell_temperaturenull
experiment.exposure_timenull
experiment.number_of_framesnull
experiment.wavelengthnull
experiment.sample_detector_distancenull
experiment.concentration_minnull
experiment.concentration_max7.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.131
experiment.s_max3.244
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].chi_square_value1.29
fits[0].p_value0.0
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fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) R51E mutant in 50 mM Tris, 50 mM NaCl, 0.5 mM Tris(2-carboxyethyl)phosphine, and 5% (v/v) glycerol, pH 7.8 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a a Pilatus3 X 2M detector. One solute concentration of 7 mg/ml was measured. The data were normalized to the intensity of the transmitted beam and radially averaged. The radially averaged scattering of the solvent-blank was subtracted.
tags[]
intensity_unitnull
experimental_mw216.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i0357.0
pddf_i0_error0.7
guinier_i0355.79
guinier_i0_error3.34
pddf_rg4.59
pddf_rg_error0.01
guinier_rg4.5
guinier_rg_error0.04
pddf_dmax14.6
pddf_dmax_errornull
porod_volume289.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last27
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDC34_datloglog_img.png
symmetrynull
last_modified2026-05-18T10:06:35.099964+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDC34
statussuccess
started_at2026-08-11T14:12:45.005942+00:00
finished_at2026-08-11T14:12:54.034346+00:00
source_last_modified2026-05-18T10:06:35.099964+02:00
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查看完整 summary.json 原文
{
  "code": "SASDC34",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Biophysical investigation of type A PutAs reveals a conserved core oligomeric structure.",
    "publication": {
      "title": "Biophysical investigation of type A PutAs reveals a conserved core oligomeric structure.",
      "author_list": "Korasick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ",
      "journal": "FEBS J",
      "doi": "10.1111/febs.14165",
      "pmid": "28710792",
      "published_date": "2017 Sep"
    },
    "status": "released",
    "submitted_date": "2017-05-19",
    "released_date": "2017-07-17"
  },
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          "organism": "Bradyrhizobium diazoefficiens",
          "uniprot_code": "Q89E26",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "dimer",
          "molecular_type": "protein",
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      "name": "Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) R51E mutant 7.0 mg/mL",
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    "contributor": [
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        "affiliation": [
          {
            "short_name": "Mizzou",
            "address": "Columbia, MO, USA",
            "full_name": "University of Missouri-Columbia",
            "webpage": "http://missouri.edu/"
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        "contributor_name": "John",
        "contributor_surname": "Tanner",
        "orcid": null
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    "concentration_method": null,
    "concentration_unit": null,
    "date": "2016-12-16",
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    "sample_detector_distance": null,
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  "experiment_description": "Synchrotron SAXS data from solutions of Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) R51E mutant in 50 mM Tris, 50 mM NaCl, 0.5 mM Tris(2-carboxyethyl)phosphine, and 5% (v/v) glycerol, pH 7.8 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a a Pilatus3 X 2M detector. One solute concentration of 7 mg/ml was measured. The data were normalized to the intensity of the transmitted beam and radially averaged. The  radially averaged scattering of the solvent-blank was subtracted.",
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  "last_modified": "2026-05-18T10:06:35.099964+02:00",
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}
查看完整 manifest.json 原文
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  "started_at": "2026-08-11T14:12:45.005942+00:00",
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