SASDC38

Truncated α-DsbA2 protein

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2021-04-10T09:53:41.823307+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Truncated α-DsbA2 protein

浓度1.25 – 5.0 缓冲液 / pH25 mM TRIS, 150 mM NaCl / 7.5
实验温度10.0 设备 / 束线Australian Synchrotron / SAXS/WAXS
波长0.1033 nm曝光2.0 s × 8

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
DsbA-like disulfide oxidoreductase (thiol-disulfide exchange protein)
查看序列
SNAARDNVTKSKISQYKDQIFDLTYPYSGNENSSVIAVGFLDYSCGHCKAIKNDIKQLINDGKIKYIFRDAPILGNASLKAAKSALAVYFLNKEKYFDFHHAALSHKGEFSDESILDIVKNIGIDEDDFNDSIKDNADKIEQMINNSRLLVRDLGVGGTPFLIIGDSLFVGATDLNVLRKKVDELSHKQG
proteinWolbachia endosymbiont of Drosophila melanogasterQ73FL665–252monomer分子数 121.091 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1296[3]0.0099553876–0.3995588124 1/A含误差列缺失 02sasbdb/entries/38/sasdc38/source/SASDC38.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)6.30.3Å
i0Guinier0.02150.00011/cm
i0P(r)0.02150.00011/cm
mwExperimental23.0—kDa
mwGuinier I(0)23.01.0kDa
mwPorod23.0—kDa
porod_volumePorod275.0—ų
rgGuinier1.870.01Å
rgP(r)1.880.01Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称25 mM TRIS, 150 mM NaCl缓冲液浓度—
pH7.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2012-02-29储存 / 测量温度4.0 / 10.0
曝光时间2.0帧数8
波长0.1033样品-探测器距离1.575
光源X-ray synchrotron探测器Pilatus 1M
机构 / 束线Australian Synchrotron / SAXS/WAXS · Melbourne, Australia
q 范围0.1 – 3.996样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
curvesasbdb/entries/38/sasdc38/source/SASDC38.datdownloaded137663145e3ccd788695e8282a82327480647bb42b318649774837e6d373183522ccd下载查看原文件源站
full_entry_zipsasbdb/entries/38/sasdc38/source/SASDC38.zipdownloaded603093c31f7b60bd6a0e5bb35694bd8377a2ad6f7e4cc121095354f59da39c4fc7bd0下载查看原文件源站
pddfsasbdb/entries/38/sasdc38/source/SASDC38.outdownloaded25117378ca6f328e5776997d8f9dd37e39ea3ffeac1dae161f2dec2f8a24a7d8f2a1e下载查看原文件源站
sascifsasbdb/entries/38/sasdc38/source/SASDC38.sascifdownloaded341601e900ac723781c910f9d5eb780c6e6aab2975449e61068f7413e55671a546aca3下载查看原文件源站
summarysasbdb/entries/38/sasdc38/source/summary.jsondownloaded9237d16c8b33e7b3942b5fff85319a2ed23aedbe2c62b084361ab1fa7b360f82ca2b下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(6 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:182 个字段值
字段路径原始值
codeSASDC38
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleThe atypical thiol-disulfide exchange protein α-DsbA2 from Wolbachia pipientis is a homotrimeric disulfide isomerase.
project.publication.titleThe atypical thiol-disulfide exchange protein α-DsbA2 from Wolbachia pipientis is a homotrimeric disulfide isomerase.
project.publication.author_listWalden PM, Whitten AE, Premkumar L, Halili MA, Heras B, King GJ, Martin JL
project.publication.journalActa Crystallogr D Struct Biol
project.publication.doi10.1107/S2059798318018442
project.publication.pmid30950399
project.publication.published_date2019 Mar 1
project.statusreleased
project.submitted_date2017-10-03
project.released_date2019-03-29
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intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDC38.dat
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experiment.instrument.detector.typeDectris
experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameAustralian Synchrotron
experiment.instrument.cityMelbourne
experiment.instrument.countryAustralia
experiment.instrument.beamline_nameSAXS/WAXS
experiment.instrument.beam_geometryPoint
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourceTrue
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameDsbA-like disulfide oxidoreductase (thiol-disulfide exchange protein)
experiment.sample.molecule[0].short_nameα-DsbA2
experiment.sample.molecule[0].sequenceSNAARDNVTKSKISQYKDQIFDLTYPYSGNENSSVIAVGFLDYSCGHCKAIKNDIKQLINDGKIKYIFRDAPILGNASLKAAKSALAVYFLNKEKYFDFHHAALSHKGEFSDESILDIVKNIGIDEDDFNDSIKDNADKIEQMINNSR LLVRDLGVGGTPFLIIGDSLFVGATDLNVLRKKVDELSHKQG
experiment.sample.molecule[0].organismWolbachia endosymbiont of Drosophila melanogaster
experiment.sample.molecule[0].uniprot_codeQ73FL6
experiment.sample.molecule[0].uniprot_range_first65
experiment.sample.molecule[0].uniprot_range_last252
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSRISFLLILIIAIASLPIINNWLSNRSQMLNDDYIGEKLDNYISRNFDKIVKTLKEESI KSSYAARDNVTKSKISQYKDQIFDLTYPYSGNENSSVIAVGFLDYSCGHCKAIKNDIKQL INDGKIKYIFRDAPILGNASLKAAKSALAVYFLDKEKYFDFHHAALSHKGEFSDESILDI VKNIGIDEDDFNDSIKDNADKIEQMINNSRLLVRDLGVGGTPFLIIGDSLFVGATDLNVL RKKVDELSHKQG
experiment.sample.molecule[0].mw21.091
experiment.sample.molecule[0].total_mw21.093
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name25 mM TRIS, 150 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameTruncated α-DsbA2 protein
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameANSTO
experiment.contributor[0].affiliation[0].addressKirrawee DC, NSW 2232, Australia
experiment.contributor[0].affiliation[0].full_nameAustralian Nuclear Science and Technology Organisation
experiment.contributor[0].affiliation[0].webpage
experiment.contributor[0].contributor_nameAndrew
experiment.contributor[0].contributor_surnameWhitten
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2012-02-29
experiment.storage_temperature4.0
experiment.cell_temperature10.0
experiment.exposure_time2.0
experiment.number_of_frames8
experiment.wavelength0.1033
experiment.sample_detector_distance1.575
experiment.concentration_min1.25
experiment.concentration_max5.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.1
experiment.s_max3.996
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].pdb_link[]
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fits[0].models[0].bead_radius1.75
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fits[0].models[0].user111
fits[0].fit_unit1/A
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fits[0].chi_square_value1.034
fits[0].p_value0.247123
fits[0].fit_residual_plotSASDC38_fit1_fitresiduals_img.png
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fits[1].softwareCORAL
fits[1].chi_square_value3.88
fits[1].p_value0.0
fits[1].fit_residual_plotSASDC38_fit2_fitresiduals_img.png
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fits[1].software_version1.1
fits[1].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version4.6
i0_calibration_standardnull
descriptionnull
experiment_descriptionX-ray synchrotron radiation scattering data from the truncated version of α-DsbA2 from Wolbachia pipientis in 25 mM TRIS 150mM NaCl pH 7.5 were collected on the SAXS/WAXS beam line of the Australian Synchrotron (Melbourne, Australia) using a 2D Photon counting Pilatus 1M-W pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). Eight successive 2 second frames were collected across solute concentrations of 1.25-5.00 mg/ml. The SAXS data displayed this entry was derived from a 1.25 mg/ml sample. The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering of the solvent-blank was subtracted. The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering of the solvent-blank was subtracted. The models and corresponding fits include those derived from dummy-atom modelling using DAMMIN (top) and rigid-body modelling using CORAL (bottom).
tags[]
intensity_unit1/cm
experimental_mw23.0
experimental_mw_errornull
guinier_i0_mw23.0
guinier_i0_mw_error1.0
porod_mw23.0
porod_mw_errornull
pddf_i00.0215
pddf_i0_error0.0001
guinier_i00.0215
guinier_i0_error0.0001
pddf_rg1.88
pddf_rg_error0.01
guinier_rg1.87
guinier_rg_error0.01
pddf_dmax6.3
pddf_dmax_error0.3
porod_volume275.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last45
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDC38_datloglog_img.png
symmetrynull
last_modified2021-04-10T09:53:41.823307+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDC38
statussuccess
started_at2026-08-11T15:44:39.400067+00:00
finished_at2026-08-11T15:44:50.889042+00:00
source_last_modified2021-04-10T09:53:41.823307+02:00
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查看完整 summary.json 原文
{
  "code": "SASDC38",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "The atypical thiol-disulfide exchange protein α-DsbA2 from Wolbachia pipientis is a homotrimeric disulfide isomerase.",
    "publication": {
      "title": "The atypical thiol-disulfide exchange protein α-DsbA2 from Wolbachia pipientis is a homotrimeric disulfide isomerase.",
      "author_list": "Walden PM, Whitten AE, Premkumar L, Halili MA, Heras B, King GJ, Martin JL",
      "journal": "Acta Crystallogr D Struct Biol",
      "doi": "10.1107/S2059798318018442",
      "pmid": "30950399",
      "published_date": "2019 Mar 1"
    },
    "status": "released",
    "submitted_date": "2017-10-03",
    "released_date": "2019-03-29"
  },
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  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDC38.dat",
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  "experiment": {
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        "resolution": 172.0
      },
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      "country": "Australia",
      "beamline_name": "SAXS/WAXS",
      "beam_geometry": "Point",
      "type_of_source": "X-ray synchrotron",
      "point_source": true,
      "line_collimation": null,
      "sample_path_length": null,
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          "oligomerization": "monomer",
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            "short_name": "ANSTO",
            "address": "Kirrawee DC, NSW 2232, Australia",
            "full_name": "Australian Nuclear Science and Technology Organisation",
            "webpage": ""
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        ],
        "contributor_name": "Andrew",
        "contributor_surname": "Whitten",
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    "concentration_unit": null,
    "date": "2012-02-29",
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      "software": "DAMMIN",
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      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDC38_fit1.fir",
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  "last_modified": "2021-04-10T09:53:41.823307+02:00",
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}
查看完整 manifest.json 原文
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