SASDC42

Ethylene Receptor 1 Cytosolic Domain

Data type: SASBDB experimental data Status: Published Curve type: Merged Last updated: 2023-06-21T07:56:39.492710+02:00

ETR1 in the absence of NDSB

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Ethylene Receptor 1 Cytosolic Domain

Concentration0.7 – 4.2 mg/mlBuffer / pH20 mM TRIS 150 mM NaCl 1mM DTT 250mM NDSB / 8.8
Experimental temperature10.0 Facility / beamlinePETRA III / EMBL P12
Wavelength1.24 nmExposure0.05 s × 20

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Ethylene Receptor 1
View sequence
DRHTILKTTLVELGRTLALEECALWMPTRTGLELQLSYTLRHQHPVEYTVPIQLPVINQVFGTSRAVKISPNSPVARLRPVSGKYMLGEVVAVRVPLLHLSNFQINDWPELSTKRYALMVLMLPSDSARQWHVHELELVEVVADQVAVALSHAAILEESMRARDLLMEQNVALDLARREAETAIRARNDFLAVMNHEMRTPMHAIIALSSLLQETELTPEQRLMVETILKSSNLLATLMNDVLDLSRLEDGSLQLELGTFNLHTLFREVLNLIKPIAVVKKLPITLNLAPDLPEFVVGDEKRLMQIILNIVGNAVKFSKQGSISVTALVTKSDTRAADFFVVPTGSHFYLRVKVKDSGAGINPQDIPKIFTKFAQTQSLATRSSGGSGLGLAISKRFVNLMEGNIWIESDGLGKGCTAIFDVKLGISERSNESKQSGIPKVPAIPRHSNFTGLKVLVMDENGVSRMVTKGLLVHLGCEVTTVSSNEECLRVVSHEHKVVFMDVCMPGVENYQIALRIHEKFTKQRHQRPLLVALSGNTDKSTKEKCMSFGLDGVLLKPVSLDNIRDVLSDLLEPRVLYEGM
proteinArabidopsis thalianaP49333158–738dimerMolecule count 264.555 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11727[3]0.0757859–4.66533 1/nmHas uncertainty columnMissing 0104sasbdb/entries/42/sasdc42/source/SASDC42.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)19.00.5nm
i0Guinier1124.025.0—
i0P(r)1164.040.0—
mwExperimental53.05.0kDa
mwGuinier I(0)53.05.0kDa
mwPorod161.224.0kDa
porod_volumePorod274.042.0nm³
rgGuinier5.51.1nm
rgP(r)5.90.6nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM TRIS 150 mM NaCl 1mM DTT 250mM NDSBBuffer concentration20.0 mM
pH8.8Additives150 mM NaCl, 1mM DTT, 250mM NDSB
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / 78367Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2013-03-29Storage / measurement temperature10.0 / 10.0
Exposure time0.05Frames20
Wavelength1.24Sample-to-detector distance3.1
SourceX-ray synchrotronDetectorPilatus 2M
Facility / beamlinePETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.076 – 4.665Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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full_entry_zip:source record and ZIP contents (4 items)
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:151 field values
Field pathRaw value
codeSASDC42
statusPublished
type_of_curveMerged
angular_unit1/nm
project.titleStructural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1).
project.publication.titleStructural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1).
project.publication.author_listMayerhofer H, Panneerselvam S, Kaljunen H, Tuukkanen A, Mertens HD, Mueller-Dieckmann J
project.publication.journalJ Biol Chem
project.publication.doi10.1074/jbc.M114.587667
project.publication.pmid25451923
project.publication.published_date2015 Jan 30
project.statusreleased
project.submitted_date2017-02-07
project.released_date2017-02-09
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameEthylene Receptor 1
experiment.sample.molecule[0].short_nameETR1(-ΔTM)
experiment.sample.molecule[0].sequenceDRHTILKTTLVELGRTLALEECA LWMPTRTGLELQLSYTLRHQHPVEYTVPIQLPVINQVFGTSRAVKISPNSPVARLRPVSG KYMLGEVVAVRVPLLHLSNFQINDWPELSTKRYALMVLMLPSDSARQWHVHELELVEVVA DQVAVALSHAAILEESMRARDLLMEQNVALDLARREAETAIRARNDFLAVMNHEMRTPMH AIIALSSLLQETELTPEQRLMVETILKSSNLLATLMNDVLDLSRLEDGSLQLELGTFNLH TLFREVLNLIKPIAVVKKLPITLNLAPDLPEFVVGDEKRLMQIILNIVGNAVKFSKQGSI SVTALVTKSDTRAADFFVVPTGSHFYLRVKVKDSGAGINPQDIPKIFTKFAQTQSLATRS SGGSGLGLAISKRFVNLMEGNIWIESDGLGKGCTAIFDVKLGISERSNESKQSGIPKVPA IPRHSNFTGLKVLVMDENGVSRMVTKGLLVHLGCEVTTVSSNEECLRVVSHEHKVVFMDV CMPGVENYQIALRIHEKFTKQRHQRPLLVALSGNTDKSTKEKCMSFGLDGVLLKPVSLDN IRDVLSDLLEPRVLYEGM
experiment.sample.molecule[0].organismArabidopsis thaliana
experiment.sample.molecule[0].uniprot_codeP49333
experiment.sample.molecule[0].uniprot_range_first158
experiment.sample.molecule[0].uniprot_range_last738
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMEVCNCIEPQWPADELLMKYQYISDFFIAIAYFSIPLELIYFVKKSAVFPYRWVLVQFGA FIVLCGATHLINLWTFTTHSRTVALVMTTAKVLTAVVSCATALMLVHIIPDLLSVKTREL FLKNKAAELDREMGLIRTQEETGRHVRMLTHEIRSTLDRHTILKTTLVELGRTLALEECA LWMPTRTGLELQLSYTLRHQHPVEYTVPIQLPVINQVFGTSRAVKISPNSPVARLRPVSG KYMLGEVVAVRVPLLHLSNFQINDWPELSTKRYALMVLMLPSDSARQWHVHELELVEVVA DQVAVALSHAAILEESMRARDLLMEQNVALDLARREAETAIRARNDFLAVMNHEMRTPMH AIIALSSLLQETELTPEQRLMVETILKSSNLLATLMNDVLDLSRLEDGSLQLELGTFNLH TLFREVLNLIKPIAVVKKLPITLNLAPDLPEFVVGDEKRLMQIILNIVGNAVKFSKQGSI SVTALVTKSDTRAADFFVVPTGSHFYLRVKVKDSGAGINPQDIPKIFTKFAQTQSLATRS SGGSGLGLAISKRFVNLMEGNIWIESDGLGKGCTAIFDVKLGISERSNESKQSGIPKVPA IPRHSNFTGLKVLVMDENGVSRMVTKGLLVHLGCEVTTVSSNEECLRVVSHEHKVVFMDV CMPGVENYQIALRIHEKFTKQRHQRPLLVALSGNTDKSTKEKCMSFGLDGVLLKPVSLDN IRDVLSDLLEPRVLYEGM
experiment.sample.molecule[0].mw64.555
experiment.sample.molecule[0].total_mw129.11
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM TRIS 150 mM NaCl 1mM DTT 250mM NDSB
experiment.sample.buffer.concentration_unitmM
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive150 mM NaCl, 1mM DTT, 250mM NDSB
experiment.sample.buffer.concentration20.0
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.8
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameEthylene Receptor 1 Cytosolic Domain
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_vol78367
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameHaydyn
experiment.contributor[0].contributor_surnameMertens
experiment.contributor[0].orcidnull
experiment.contributor[1].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[1].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[1].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[1].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[1].contributor_nameAnne
experiment.contributor[1].contributor_surnameTuukkanen
experiment.contributor[1].orcidnull
experiment.concentration_methodA280
experiment.concentration_unitmg/ml
experiment.date2013-03-29
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time0.05
experiment.number_of_frames20
experiment.wavelength1.24
experiment.sample_detector_distance3.1
experiment.concentration_min0.7
experiment.concentration_max4.2
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.076
experiment.s_max4.665
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[0].models[]
fits[0].fit_unit1/A
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fits[0].softwareEOM/GAJOE
fits[0].chi_square_value1.4641
fits[0].p_value0.0
fits[0].fit_residual_plotfit4columns1262_fitresiduals_img.png
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fits[0].software_version2.0
fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionGNOM
i0_calibration_standardBSA
descriptionETR1 in the absence of NDSB
experiment_descriptionnull
tags[]
intensity_unitnull
experimental_mw53.0
experimental_mw_error5.0
guinier_i0_mw53.0
guinier_i0_mw_error5.0
porod_mw161.2
porod_mw_error24.0
pddf_i01164.0
pddf_i0_error40.0
guinier_i01124.0
guinier_i0_error25.0
pddf_rg5.9
pddf_rg_error0.6
guinier_rg5.5
guinier_rg_error1.1
pddf_dmax19.0
pddf_dmax_error0.5
porod_volume274.0
porod_volume_error42.0
estimated_volumenull
estimated_volume_errornull
guinier_point_first19
guinier_point_last42
pddf_point_first19
pddf_point_last500
i0_calibration_standard_datanull
intensities_log_log_plotSASDC42_datloglog_img.png
symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDC42
statussuccess
started_at2026-08-11T13:29:52.760345+00:00
finished_at2026-08-11T13:30:01.738437+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
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View the complete summary.json source
{
  "code": "SASDC42",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/nm",
  "project": {
    "title": "Structural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1).",
    "publication": {
      "title": "Structural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1).",
      "author_list": "Mayerhofer H, Panneerselvam S, Kaljunen H, Tuukkanen A, Mertens HD, Mueller-Dieckmann J",
      "journal": "J Biol Chem",
      "doi": "10.1074/jbc.M114.587667",
      "pmid": "25451923",
      "published_date": "2015 Jan 30"
    },
    "status": "released",
    "submitted_date": "2017-02-07",
    "released_date": "2017-02-09"
  },
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      "country": "Germany",
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    },
    "sample": {
      "molecule": [
        {
          "long_name": "Ethylene Receptor 1",
          "short_name": "ETR1(-ΔTM)",
          "sequence": "DRHTILKTTLVELGRTLALEECA\r\nLWMPTRTGLELQLSYTLRHQHPVEYTVPIQLPVINQVFGTSRAVKISPNSPVARLRPVSG\r\nKYMLGEVVAVRVPLLHLSNFQINDWPELSTKRYALMVLMLPSDSARQWHVHELELVEVVA\r\nDQVAVALSHAAILEESMRARDLLMEQNVALDLARREAETAIRARNDFLAVMNHEMRTPMH\r\nAIIALSSLLQETELTPEQRLMVETILKSSNLLATLMNDVLDLSRLEDGSLQLELGTFNLH\r\nTLFREVLNLIKPIAVVKKLPITLNLAPDLPEFVVGDEKRLMQIILNIVGNAVKFSKQGSI\r\nSVTALVTKSDTRAADFFVVPTGSHFYLRVKVKDSGAGINPQDIPKIFTKFAQTQSLATRS\r\nSGGSGLGLAISKRFVNLMEGNIWIESDGLGKGCTAIFDVKLGISERSNESKQSGIPKVPA\r\nIPRHSNFTGLKVLVMDENGVSRMVTKGLLVHLGCEVTTVSSNEECLRVVSHEHKVVFMDV\r\nCMPGVENYQIALRIHEKFTKQRHQRPLLVALSGNTDKSTKEKCMSFGLDGVLLKPVSLDN\r\nIRDVLSDLLEPRVLYEGM",
          "organism": "Arabidopsis thaliana",
          "uniprot_code": "P49333",
          "uniprot_range_first": 158,
          "uniprot_range_last": 738,
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View the complete manifest.json source
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