SASDC46

PH - Pleckstrin-homology domain of Bcr-Abl tyrosine kinase p210

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Merged 最后更新:2023-06-21T07:56:39.492710+02:00

The homology model is derived from PDB ID: 2z0q. The PDB entry for BCR-ABL p210 fusion protein PH domain is 5oc7 (has an internal deletion).

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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · PH - Pleckstrin-homology domain of Bcr-Abl tyrosine kinase p210

浓度1.0 – 9.14 缓冲液 / pH25 mM Tris-HCl, 150 mM NaCl, 5% Glycerol, 1 mM DTT / 7.5
实验温度10.0 设备 / 束线PETRA III / EMBL P12
波长0.124 nm曝光0.045 s × 20

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
BCR-ABL p210 fusion protein (PH domain)
查看序列
GAMGEHRQLLKDSFMVELVEGARKLRHVFLFTDLLLCTKLKKQSGGKTQQYDCKWYIPLTDLSFQMVDELEAVPNIPLVPDEELDALKIKISQIKNDIQREKRANKGSKATERLKKKLSEQESLLLLMSPSMAFRVHSRNGKSYTFLISSDYERAEWRENIREQQKKCFRSFSLTSVELQMLTNSCVKLQTVH
proteinHomo sapiensP11274—–—monomer分子数 122.413 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11809[3]0.00214005–0.378991 1/A含误差列缺失 016sasbdb/entries/46/sasdc46/source/SASDC46.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)6.60.3Å
i0Guinier2071.99.84—
i0P(r)2074.09.0—
mwExperimental23.0—kDa
mwGuinier I(0)16.03.0kDa
mwPorod23.0—kDa
porod_volumePorod38.0—ų
rgGuinier2.040.03Å
rgP(r)2.070.02Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

拟合 1 · SREFLEX 2.8.1

χ²:1.1CorMap p:0.026281单位:1/A
模型名称 / 类型软件对称性模型 MWPDB 声明来源文件
1—atomicSREFLEX 2.8.1—19.02—模型 图片
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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称25 mM Tris-HCl, 150 mM NaCl, 5% Glycerol, 1 mM DTT缓冲液浓度—
pH7.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2016-11-10储存 / 测量温度10.0 / 10.0
曝光时间0.045帧数20
波长0.124样品-探测器距离3.0
光源X-ray synchrotron探测器Pilatus 2M
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q 范围0.021 – 3.79样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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pddfsasbdb/entries/46/sasdc46/source/SASDC46.outdownloaded1279961317fc56d7804318053883376e34f5f03c4f1c785118e5b99b2a1517f785f909下载查看原文件源站
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summarysasbdb/entries/46/sasdc46/source/summary.jsondownloaded8173d39f01528ed12e7f7365a694ab2cf54a638d7351f751ce95d6d80bbd5c55bb89下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDC46
statusPublished
type_of_curveMerged
angular_unit1/A
project.titleStructural and functional dissection of the DH and PH domains of oncogenic Bcr-Abl tyrosine kinase.
project.publication.titleStructural and functional dissection of the DH and PH domains of oncogenic Bcr-Abl tyrosine kinase.
project.publication.author_listReckel S, Gehin C, Tardivon D, Georgeon S, Kükenshöner T, Löhr F, Koide A, Buchner L, Panjkovich A, Reynaud A, Pinho S, Gerig B, Svergun D, Pojer F, Güntert P, Dötsch V, Koide S, Gavin AC, Hantschel O
project.publication.journalNat Commun
project.publication.doi10.1038/s41467-017-02313-6
project.publication.pmid29235475
project.publication.published_date2017 Dec 13
project.statusreleased
project.submitted_date2017-07-10
project.released_date2018-01-03
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intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDC46.dat
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameBCR-ABL p210 fusion protein (PH domain)
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceGAMGEHRQLLKDSFMVELVEGAR KLRHVFLFTDLLLCTKLKKQ SGGKTQQYDCKWYIPLTDLS FQMVDELEAVPNIPLVPDEE LDALKIKISQIKNDIQREKR ANKGSKATERLKKKLSEQES LLLLMSPSMAFRVHSRNGKS YTFLISSDYERAEWRENIRE QQKKCFRSFSLTSVELQMLT NSCVKLQTVH
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP11274
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.molecule[0].mw22.413
experiment.sample.molecule[0].total_mw22.413
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name25 mM Tris-HCl, 150 mM NaCl, 5% Glycerol, 1 mM DTT
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.namePH - Pleckstrin-homology domain of Bcr-Abl tyrosine kinase p210
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameAlejandro
experiment.contributor[0].contributor_surnamePanjkovich
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2016-11-10
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time0.045
experiment.number_of_frames20
experiment.wavelength0.124
experiment.sample_detector_distance3.0
experiment.concentration_min1.0
experiment.concentration_max9.14
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.021
experiment.s_max3.79
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].bead_radius1.9
fits[0].models[0].lognull
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fits[0].models[0].comment
fits[0].models[0].user145
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fits[0].softwareSREFLEX
fits[0].chi_square_value1.1
fits[0].p_value0.026281
fits[0].fit_residual_plotSASDC46_fit1_fitresiduals_img.png
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fits[0].fit_lognull
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fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionThe homology model is derived from PDB ID: 2z0q. The PDB entry for BCR-ABL p210 fusion protein PH domain is 5oc7 (has an internal deletion).
experiment_descriptionnull
tags[]
intensity_unitnull
experimental_mw23.0
experimental_mw_errornull
guinier_i0_mw16.0
guinier_i0_mw_error3.0
porod_mw23.0
porod_mw_errornull
pddf_i02074.0
pddf_i0_error9.0
guinier_i02071.9
guinier_i0_error9.84
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pddf_rg_error0.02
guinier_rg2.04
guinier_rg_error0.03
pddf_dmax6.6
pddf_dmax_error0.3
porod_volume38.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first26
guinier_point_last296
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDC46_datloglog_img.png
symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDC46
statussuccess
started_at2026-08-11T15:01:18.511740+00:00
finished_at2026-08-11T15:01:28.646641+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
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查看完整 summary.json 原文
{
  "code": "SASDC46",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/A",
  "project": {
    "title": "Structural and functional dissection of the DH and PH domains of oncogenic Bcr-Abl tyrosine kinase.",
    "publication": {
      "title": "Structural and functional dissection of the DH and PH domains of oncogenic Bcr-Abl tyrosine kinase.",
      "author_list": "Reckel S, Gehin C, Tardivon D, Georgeon S, Kükenshöner T, Löhr F, Koide A, Buchner L, Panjkovich A, Reynaud A, Pinho S, Gerig B, Svergun D, Pojer F, Güntert P, Dötsch V, Koide S, Gavin AC, Hantschel O",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-017-02313-6",
      "pmid": "29235475",
      "published_date": "2017 Dec 13"
    },
    "status": "released",
    "submitted_date": "2017-07-10",
    "released_date": "2018-01-03"
  },
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    "sample": {
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        {
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          "uniprot_code": "P11274",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
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          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
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      ],
      "buffer": {
        "name": "25 mM Tris-HCl, 150 mM NaCl, 5% Glycerol, 1 mM DTT",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
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      "purity_method": null,
      "name": "PH - Pleckstrin-homology domain of Bcr-Abl tyrosine kinase p210",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "EMBL-Hamburg",
            "address": "Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany",
            "full_name": "European Molecular Biology Laboratory (EMBL) - Hamburg outstation",
            "webpage": "http://www.embl-hamburg.de/index.php"
          }
        ],
        "contributor_name": "Alejandro",
        "contributor_surname": "Panjkovich",
        "orcid": null
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    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2016-11-10",
    "storage_temperature": 10.0,
    "cell_temperature": 10.0,
    "exposure_time": 0.045,
    "number_of_frames": 20,
    "wavelength": 0.124,
    "sample_detector_distance": 3.0,
    "concentration_min": 1.0,
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    "sample_volume": null,
    "flow_rate": null,
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    "total_exposure_time": null,
    "seccolumn": null
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          "software": "SREFLEX",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "atomic",
          "software_version": "2.8.1",
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDC46_fit1_model1.pdb",
          "model_mw": 19.02,
          "bead_radius": 1.9,
          "log": null,
          "symmetry": "",
          "comment": "",
          "user": 145
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      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDC46_fit1_fit_img.png",
      "software": "SREFLEX",
      "chi_square_value": 1.1,
      "p_value": 0.026281,
      "fit_residual_plot": "SASDC46_fit1_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDC46_fit1.fit",
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  "description": "The homology model is derived from PDB ID: 2z0q. The PDB entry for BCR-ABL p210 fusion protein PH domain is 5oc7 (has an internal deletion).",
  "experiment_description": null,
  "tags": [],
  "intensity_unit": null,
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  "intensities_log_log_plot": "SASDC46_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2023-06-21T07:56:39.492710+02:00",
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}
查看完整 manifest.json 原文
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