SASDC47

Saposin nanoparticle (SapNP) SapA:POPG (SEC-SAXS)

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2023-06-21T07:56:39.492710+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Saposin nanoparticle (SapNP) SapA:POPG (SEC-SAXS)

Concentration— – 7.1 Buffer / pHPBS / 7.4
Experimental temperature10.0 Facility / beamlinePETRA III / EMBL P12
Wavelength0.124 nmExposure1.0 s × 3600

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
saposin-a
View sequence
SMSLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCES
proteinHomo sapiensP0760260–140monomerMolecule count 19.084 kDa
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol)othersynthetic construct——–—otherMolecule count —0.77 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11796[3]0.0879751–5.00746 1/nmHas uncertainty columnMissing 0264sasbdb/entries/47/sasdc47/source/SASDC47.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)10.50.5nm
i0Guinier818.03.0arbitrary
i0P(r)823.04.0arbitrary
mwExperimental42.04.0kDa
mwPorod43.04.0kDa
porod_volumePorod69.07.0nm³
rgGuinier4.10.1nm
rgP(r)4.00.1nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

▼

3. Source Fits and Models Source Fits & Models

Fit 1 · DAMMIF 2.9.0

χ²: 1.3CorMap p: 0.049646Unit: 1/A
ModelName / TypeSoftwareSymmetryModel MWPDB DeclarationSource file
1—dummyDAMMIF 2.9.0P1——Model Image
▶

4. Source File Index Source Files

▶

5. Experiment Description and Publication Experiment & Publication

▼

6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer namePBSBuffer concentration—
pH7.4Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuterationTrue / —

Acquisition Conditions and Instrument

Measurement date2016-12-15Storage / measurement temperature10.0 / 10.0
Exposure time1.0Frames3600
Wavelength0.124Sample-to-detector distance3.1
SourceX-ray synchrotronDetectorPilatus 2M
Facility / beamlinePETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.088 – 5.007Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:173 field values
Field pathRaw value
codeSASDC47
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleSaposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research.
project.publication.titleSaposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research.
project.publication.author_listFlayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
project.publication.journalStructure
project.publication.doi10.1016/j.str.2018.01.007
project.publication.pmid29413323
project.publication.published_date2018 Feb 6
project.statusreleased
project.submitted_date2017-08-09
project.released_date2018-02-07
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experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_namesaposin-a
experiment.sample.molecule[0].short_namesapA
experiment.sample.molecule[0].sequenceSM SLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCES
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP07602
experiment.sample.molecule[0].uniprot_range_first60
experiment.sample.molecule[0].uniprot_range_last140
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.molecule[0].mw9.084
experiment.sample.molecule[0].total_mw9.084
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_name1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol)
experiment.sample.molecule[1].short_namePOPG
experiment.sample.molecule[1].sequencenull
experiment.sample.molecule[1].organismsynthetic construct
experiment.sample.molecule[1].uniprot_codenull
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experiment.sample.molecule[1].uniprot_range_lastnull
experiment.sample.molecule[1].oligomerizationother
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experiment.sample.molecule[1].uniprot_sequencenull
experiment.sample.molecule[1].mw0.77
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experiment.sample.molecule[1].number_molecules0
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
experiment.sample.buffer.namePBS
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pka7.0
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameSaposin nanoparticle (SapNP) SapA:POPG (SEC-SAXS)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixtureTrue
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameHaydyn
experiment.contributor[0].contributor_surnameMertens
experiment.contributor[0].orcidnull
experiment.concentration_methodA280
experiment.concentration_unitnull
experiment.date2016-12-15
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time1.0
experiment.number_of_frames3600
experiment.wavelength0.124
experiment.sample_detector_distance3.1
experiment.concentration_minnull
experiment.concentration_max7.1
experiment.sample_volumenull
experiment.flow_ratenull
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experiment.s_max5.007
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experiment.seccolumnnull
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experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw42.0
experimental_mw_error4.0
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw43.0
porod_mw_error4.0
pddf_i0823.0
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guinier_i0818.0
guinier_i0_error3.0
pddf_rg4.0
pddf_rg_error0.1
guinier_rg4.1
guinier_rg_error0.1
pddf_dmax10.5
pddf_dmax_error0.5
porod_volume69.0
porod_volume_error7.0
estimated_volumenull
estimated_volume_errornull
guinier_point_first32
guinier_point_last110
pddf_point_first33
pddf_point_last1000
i0_calibration_standard_datanull
intensities_log_log_plotSASDC47_datloglog_img.png
symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDC47
statussuccess
started_at2026-08-11T15:21:30.524924+00:00
finished_at2026-08-11T15:21:40.952837+00:00
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View the complete summary.json source
{
  "code": "SASDC47",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research.",
    "publication": {
      "title": "Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research.",
      "author_list": "Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C",
      "journal": "Structure",
      "doi": "10.1016/j.str.2018.01.007",
      "pmid": "29413323",
      "published_date": "2018 Feb 6"
    },
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View the complete manifest.json source
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