SASDC58

Dipeptidyl peptidase III: pgDPP3_FL

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Merged 最后更新:2019-12-06T13:07:15.624532+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Dipeptidyl peptidase III: pgDPP3_FL

浓度1.02 – 8.8 缓冲液 / pH50mM Tris,100mM NaCl / 8.0
实验温度4.0 设备 / 束线ESRF / BM29
波长0.099 nm曝光— s × 8

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Peptidase, M49 family
查看序列
MTKETTQHRSGERVARFADIEVLSYRADLFGTLTPKQRMLCYHLSEAALRGRDITTIQNCRYNLWVRSLMERIYTHLSKSERTDDFALLEEYLFCIWFANGIHHHYSGAKFIARFSPEFLREALRVTGVELEPEEQALLERVLYDTDFLPKQTEQSGEEDIIKASSVNFYAPGITRAEAESHYKNLIEALPENERSCPPSFGLNTRLIRSTSGELKDEVCCIDGLYSPAIEAVVASLEAAIPYTENEEQAACIRLLCDYYRTGDVRLYDRFCIRWVENNRTRIDFINGFTEVYADPIGIHGSWEGLVHMQDEEAGRRTRIISEHAGWFEAHSPIDARFRKKNPHGISATVVNVLTIAGDSYPATPIGINLPNADWIRAEHGSKSVTIDNITDAYNHAARGTGLYEEFIPDEEVRRHVELHADLTDSLHTDLHECLGHGSGQLLPGVPGDALGEHASTLEETRADLFALYFLADPKMIELGLLTDPDAYKANYYKYMLNGLMTQLVRIKRGEEIEEAHMRNRALIARYVLEHAERPGAMSLVCEEGKTALVIKDYEAVRAIIAGLLTEVQRIKSEGDYTAGKALVERYAVHVDPLLHEEVLMRYAKLDIAPYKGFVNPRLRPVYNSEGRLTDATIEYTEGYAEQMLRYSAEYSFLPTDSPLLQEARRLRSHLRRAMDGVLSASMREKGLHYGINFGVTREHLLRLARTADASAPLADYLWRRDVRETKILATMIFPAEELTHEQATRFLREADNVELREQLTANLLERMPEAIRSIGRWIESKETTPDMMTGVLTLAARLFTRGIFPENAPAEKLLALAILHLSDEEQKTELRRASALLLKRYGRGSAERTKKVLCLLPESSQDTAPVLYELCEDIRFELDFYPKDEHHHHHH
proteinPorphyromonas gingivalisQ7MX92—–—monomer分子数 1101.655 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1989[3]0.1332806–4.818673 1/nm含误差列缺失 00sasbdb/entries/58/sasdc58/source/SASDC58.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)11.25—nm
i0Guinier70.2440.289arbitrary
i0P(r)71.020.185arbitrary
mwExperimental101.0—kDa
mwGuinier I(0)101.0—kDa
mwPorod72.0—kDa
porod_volumePorod116.0—nm³
rgGuinier3.00.018nm
rgP(r)3.080.012nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50mM Tris,100mM NaCl缓冲液浓度—
pH8.0添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2015-03-14储存 / 测量温度4.0 / 4.0
曝光时间—帧数8
波长0.099样品-探测器距离2.5
光源X-ray synchrotron探测器Pilatus 1M
机构 / 束线ESRF / BM29 · Grenoble, France
q 范围0.133 – 4.819样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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pddfsasbdb/entries/58/sasdc58/source/SASDC58.outdownloaded39411b83481587bf0f168b5497334d86143e9e0af83fff544463c5950340b687898e5下载查看原文件源站
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summarysasbdb/entries/58/sasdc58/source/summary.jsondownloaded8994fcdf383d189c141ea51f0807aff450c5faeac97d6cff0185edf4f09c3342af91下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDC58
statusPublished
type_of_curveMerged
angular_unit1/nm
project.titleA novel Porphyromonas gingivalis enzyme: An atypical dipeptidyl peptidase III with an ARM repeat domain.
project.publication.titleA novel Porphyromonas gingivalis enzyme: An atypical dipeptidyl peptidase III with an ARM repeat domain.
project.publication.author_listHromić-Jahjefendić A, Jajčanin Jozić N, Kazazić S, Grabar Branilović M, Karačić Z, Schrittwieser JH, Das KMP, Tomin M, Oberer M, Gruber K, Abramić M, Tomić S
project.publication.journalPLoS One
project.publication.doi10.1371/journal.pone.0188915
project.publication.pmid29190734
project.publication.published_date2017
project.statusreleased
project.submitted_date2017-10-09
project.released_date2017-12-12
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intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDC58.dat
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experiment.instrument.detector.typeDectris
experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_namePeptidase, M49 family
experiment.sample.molecule[0].short_namePgDPP3
experiment.sample.molecule[0].sequenceMTKETTQHRSGERVARFADIEVLSYRADLFGTLTPKQRMLCYHLSEAALRGRDITTIQNCRYNLWVRSLMERIYTHLSKSERTDDFALLEEYLFCIWFANGIHHHYSGAKFIARFSPEFLREALRVTGVELEPEEQALLERVLYDTDFLPKQTEQSGEEDIIKASSVNFYAPGITRAEAESHYKNLIEALPENERSCPPSFGLNTRLIRSTSGELKDEVCCIDGLYSPAIEAVVASLEAAIPYTENEEQAACIRLLCDYYRTGDVRLYDRFCIRWVENNRTRIDFINGFTEVYADPIGIHGSWEGLVHMQDEEAGRRTRIISEHAGWFEAHSPIDARFRKKNPHGISATVVNVLTIAGDSYPATPIGINLPNADWIRAEHGSKSVTIDNITDAYNHAARGTGLYEEFIPDEEVRRHVELHADLTDSLHTDLHECLGHGSGQLLPGVPGDALGEHASTLEETRADLFALYFLADPKMIELGLLTDPDAYKANYYKYMLNGLMTQLVRIKRGEEIEEAHMRNRALIARYVLEHAERPGAMSLVCEEGKTALVIKDYEAVRAIIAGLLTEVQRIKSEGDYTAGKALVERYAVHVDPLLHEEVLMRYAKLDIAPYKGFVNPRLRPVYNSEGRLTDATIEYTEGYAEQMLRYSAEYSFLPTDSPLLQEARRLRSHLRRAMDGVLSASMREKGLHYGINFGVTREHLLRLARTADASAPLADYLWRRDVRETKILATMIFPAEELTHEQATRFLREADNVELREQLTANLLERMPEAIRSIGRWIESKETTPDMMTGVLTLAARLFTRGIFPENAPAEKLLALAILHLSDEEQKTELRRASALLLKRYGRGSAERTKKVLCLLPESSQDTAPVLYELCEDIRFELDFYPKDEHHHHHH
experiment.sample.molecule[0].organismPorphyromonas gingivalis
experiment.sample.molecule[0].uniprot_codeQ7MX92
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMTKETTQHRSGERIARFADIEVLSYRADLFGTLTPKQRMLCYHLSEAALRGRDITTIQNC RYNLWVRSLMERIYTHLSKSERTDDFALLEEYLFCIWFANGIHHHYSGAKFIARFSPGFL RAALREAGVELEPEEQVLLERVLYDTDFLPKQTEQSGEEDIIKASSVNFYAPGITRAEAE SHYKNLIEALPENEKSCPPSFGLNTRLIRSTSGELKDEVCCIDGLYGPAIEAVVASLEAA IPYTENEEQAACIRLLCDYYRTGDIRLYDRFCIRWVENNRTRIDFINGFTEVYADPIGIH GSWEGLVHMQDEEAGRRTRIISEHAGWFEAHSPIDARFRKKNPHGISATVVNVLTIAGDS YPATPIGINLPNADWIRAEHGSKSVTIDNITDAYNHAARGTGLYEEFIPDEEVRRHVELH ADLTDSLHTDLHECLGHGSGQLLPGVPGDALGEHASTLEETRADLFALYFLADPKMIELG LLTDPDAYKANYYKYMLNGLMTQLVRIKRGEEIEEAHMRNRALIARYVLEHAERPGAMSL VCEEGKTALVIKDYEAVRAIIAGLLTEVQRIKSEGDYTAGKALVERYAVHVDPLLHEEVL TRYAKLDIAPYKGFVNPRLRPVYNSEGRLTDATIEYTEGYAEQMLRYSAEYSFLPTDSPL LQEARRLRSHLRRAMDGVLSASMREKGLHYGINFGVTREHLLRLARTADASAPLADYLWR RDVRETKILATMIFPAEELTHEQATRLLREADNVELREQLTANLLERMPEAIRSIGRWIE SKETTPDMMTGVLTLAARLFTRGIFPEDVPAEKLLTLAILHLSDEEQKAELRRASALLLK RYGRGSAERTKKVLCLLPESSQDTAPVLYELCEDIRFELDFYPKDE
experiment.sample.molecule[0].mw101.655
experiment.sample.molecule[0].total_mw101.655
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name50mM Tris,100mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameDipeptidyl peptidase III: pgDPP3_FL
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressGraz, Austria
experiment.contributor[0].affiliation[0].full_nameUniversity of Graz
experiment.contributor[0].affiliation[0].webpagehttps://www.uni-graz.at
experiment.contributor[0].contributor_nameKrishna Mohan
experiment.contributor[0].contributor_surnamePadmanabha Das
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2015-03-14
experiment.storage_temperature4.0
experiment.cell_temperature4.0
experiment.exposure_timenull
experiment.number_of_frames8
experiment.wavelength0.099
experiment.sample_detector_distance2.5
experiment.concentration_min1.02
experiment.concentration_max8.8
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.133
experiment.s_max4.819
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].softwareDAMMIN
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fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modeldummy
fits[0].models[0].software_version
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fits[0].models[0].model_mw62.63
fits[0].models[0].bead_radius3.4
fits[0].models[0].loghttps://www.sasbdb.org/media/log_files/pgDPP3_1.log
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fits[0].models[0].comment
fits[0].models[0].user244
fits[0].fit_unit1/A
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fits[0].softwareDAMMIN
fits[0].chi_square_value0.636
fits[0].p_value0.052255
fits[0].fit_residual_plotSASDC58_fit1_fitresiduals_img.png
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fits[0].software_version
fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of Dipeptidyl peptidase III: pgDPP3_FL in 50mM Tris,100mM NaCl, pH 8, were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.5 m (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.0 and 8.8 mg/ml were measured . Eight successive frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
tags[]
intensity_unitarbitrary
experimental_mw101.0
experimental_mw_errornull
guinier_i0_mw101.0
guinier_i0_mw_errornull
porod_mw72.0
porod_mw_errornull
pddf_i071.02
pddf_i0_error0.185
guinier_i070.244
guinier_i0_error0.289
pddf_rg3.08
pddf_rg_error0.012
guinier_rg3.0
guinier_rg_error0.018
pddf_dmax11.25
pddf_dmax_errornull
porod_volume116.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first22
guinier_point_last64
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDC58_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDC58
statussuccess
started_at2026-08-11T15:46:03.186239+00:00
finished_at2026-08-11T15:46:11.417726+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
{
  "code": "SASDC58",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/nm",
  "project": {
    "title": "A novel Porphyromonas gingivalis enzyme: An atypical dipeptidyl peptidase III with an ARM repeat domain.",
    "publication": {
      "title": "A novel Porphyromonas gingivalis enzyme: An atypical dipeptidyl peptidase III with an ARM repeat domain.",
      "author_list": "Hromić-Jahjefendić A, Jajčanin Jozić N, Kazazić S, Grabar Branilović M, Karačić Z, Schrittwieser JH, Das KMP, Tomin M, Oberer M, Gruber K, Abramić M, Tomić S",
      "journal": "PLoS One",
      "doi": "10.1371/journal.pone.0188915",
      "pmid": "29190734",
      "published_date": "2017"
    },
    "status": "released",
    "submitted_date": "2017-10-09",
    "released_date": "2017-12-12"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDC58.out",
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            "address": "Graz, Austria",
            "full_name": "University of Graz",
            "webpage": "https://www.uni-graz.at"
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        "contributor_name": "Krishna Mohan",
        "contributor_surname": "Padmanabha Das",
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}
查看完整 manifest.json 原文
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