2. Measurements Reported by SASBDB Reported Results
Metric
Method
Value
Uncertainty
Unit
dmax
P(r)
8.0
—
Å
i0
Guinier
0.85
0.014
—
i0
P(r)
0.86
0.006
—
mw
Experimental
28.5
—
kDa
rg
Guinier
2.48
0.05
Å
rg
P(r)
2.55
0.017
Å
These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.
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3. Source Fits and Models Source Fits & Models
This entry has no source fit record.
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4. Source File Index Source Files
Category
Path
Download status
Size
curve
sasbdb/entries/74/sasdc74/source/SASDC74.dat
downloaded
7300
full_entry_zip
sasbdb/entries/74/sasdc74/source/SASDC74.zip
downloaded
9945
pddf
sasbdb/entries/74/sasdc74/source/SASDC74.out
downloaded
27718
sascif
sasbdb/entries/74/sasdc74/source/SASDC74.sascif
downloaded
39011
summary
sasbdb/entries/74/sasdc74/source/summary.json
downloaded
5933
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5. Experiment Description and Publication Experiment & Publication
Original Experiment Description
Synchrotron SAXS data from solutions of truncated P5abc subdomain from tetrahymena ribozyme (Time-resolved 0ms) in 10mM MOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH 7, were collected on the G1 beam line at the Cornell High Energy Synchrotron Source (CHESS; Ithaca, NY, USA) using a CCD Finger Lakes CCD detector at a sample-detector distance of 1.7 m and at a wavelength of λ = 0.109 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). 50 successive 5 second frames were collected at 20°C from a sample at 1.5 mg/ml. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Related Publication
Title
Revealing the distinct folding phases of an RNA three-helix junction.
Authors
Plumridge A, Katz AM, Calvey GD, Elber R, Kirmizialtin S, Pollack L
DOI / PMID
10.1093/nar/gky363 / 29762712
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6. Complete Source Record Complete Source Record
The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.
Synchrotron SAXS data from solutions of truncated P5abc subdomain from tetrahymena ribozyme (Time-resolved 0ms) in 10mM MOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH 7, were collected on the G1 beam line at the Cornell High Energy Synchrotron Source (CHESS; Ithaca, NY, USA) using a CCD Finger Lakes CCD detector at a sample-detector distance of 1.7 m and at a wavelength of λ = 0.109 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). 50 successive 5 second frames were collected at 20°C from a sample at 1.5 mg/ml. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.