SASDCA5

Light encoded DNA biosensor: e14B DNA

Data type: SASBDB experimental data Status: Published Curve type: Merged Last updated: 2019-12-06T13:07:15.624532+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Light encoded DNA biosensor: e14B DNA

Concentration— – 0.5 Buffer / pH154 mM NaCl / 8.3
Experimental temperature10.0 Facility / beamlineESRF / BM29
Wavelength0.1 nmExposure0.05 s × 20

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
e14B
View sequence
CACCTCGAAATCTGAAGGGCTTTTGAACTCTGTTTTCGAGGTG
DNA———–—monomerMolecule count 113.319 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11009[3]0.195053–4.95184 1/nmHas uncertainty columnMissing 069sasbdb/entries/a5/sasdca5/source/SASDCA5.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)9.0—nm
i0Guinier25.440.12—
i0P(r)26.21——
mwExperimental12.0—kDa
rgGuinier1.850.05nm
rgP(r)2.05—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name154 mM NaClBuffer concentration—
pH8.3Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2016-06-16Storage / measurement temperature10.0 / 10.0
Exposure time0.05Frames20
Wavelength0.1Sample-to-detector distance3.0
SourceX-ray synchrotronDetectorPilatus 1M
Facility / beamlineESRF / BM29 · Grenoble, France
q range0.195 – 4.952Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:156 field values
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statusPublished
type_of_curveMerged
angular_unit1/nm
project.titleOptical and Structural Characterization of a Chronic Myeloid Leukemia DNA Biosensor.
project.publication.titleOptical and Structural Characterization of a Chronic Myeloid Leukemia DNA Biosensor.
project.publication.author_listCordeiro M, Otrelo-Cardoso AR, Svergun DI, Konarev PV, Lima JC, Santos-Silva T, Baptista PV
project.publication.journalACS Chem Biol
project.publication.doi10.1021/acschembio.8b00029
project.publication.pmid29562136
project.publication.published_date2018 May 18
project.statusreleased
project.submitted_date2017-06-23
project.released_date2018-04-03
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experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
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experiment.sample.molecule[0].long_namee14B
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequencecacctcgaaatctgaagggcttttgaactctgttttcgaggtg
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experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_namePetr
experiment.contributor[0].contributor_surnameKonarev
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
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experiment.storage_temperature10.0
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experiment.exposure_time0.05
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experiment_descriptionSynchrotron SAXS data from solutions of Light encoded DNA biosensor: e14B DNA in 154 mM NaCl, pH 8.3 were collected at the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 0.50 mg/ml was measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve. The model depicts the averaged spatial representation of the DNA (DAMFILT occupancy and volume-corrected bead model).
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statussuccess
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View the complete summary.json source
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  "experiment_description": "Synchrotron SAXS data from solutions of Light encoded DNA biosensor: e14B DNA in 154 mM NaCl, pH 8.3 were collected at the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 0.50 mg/ml was measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve. The model depicts the averaged spatial representation of the DNA (DAMFILT occupancy and volume-corrected bead model).",
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  "i0_calibration_standard_data": null,
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  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
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}
View the complete manifest.json source
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