SASDCE3

Nucleolysin TIA-1 isoform p40 in complex with U15 RNA (LPQTG containing construct for sortase mediated protein ligation)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Merged 最后更新:2023-12-14T13:41:02.534142+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Nucleolysin TIA-1 isoform p40 in complex with U15 RNA (LPQTG containing construct for sortase mediated protein ligation)

浓度1.0 – 5.0 缓冲液 / pH10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT / 6.0
实验温度20.0 设备 / 束线SFB 1035, Technische Universität München / Rigaku BioSAXS-1000
波长0.1542 nm曝光900.0 s × 8

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
poly U 15mer
查看序列
UUUUUUUUUUUUUUU
RNA———–—monomer分子数 14.611 kDa
Nucleolysin TIA-1 isoform p40
查看序列
MEDEMPKTLYVGNLSRDVTEALILQLFSQIGPCKNCKMIMDTAGNDPYCFVEFHEHRHAAAALAAMNGRKIMGKEVKVNWATTPSSQKLPQTGNHFHVFVGDLSPEITTEDIKAAFAPFGRISDARVVKDMATGKSKGYGFVSFFNKWDAENAIQQMGGQWLGGRQIRTNWATRKPPAPKSTYESNTKQLSYDEVVNQSSPSNCTVYCGGVTSGLTEQLMRQTFSPFGQIMEIRVFPDKGYSFVRFNSHESAAHAIVSVNGTTIEGHVVKCYWGK
proteinHomo sapiensP31483—–—monomer分子数 130.522 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1466[3]0.02086–0.685254 1/A含误差列缺失 00sasbdb/entries/e3/sasdce3/source/SASDCE3.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)8.6—Å
i0Guinier0.03780.0001—
i0P(r)0.0380.0002—
mwExperimental49.4—kDa
mwGuinier I(0)49.4—kDa
porod_volumePorod40.0—ų
rgGuinier2.410.01Å
rgP(r)2.420.01Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT缓冲液浓度—
pH6.0添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2015-12-01储存 / 测量温度— / 20.0
曝光时间900.0帧数8
波长0.1542样品-探测器距离—
光源X-ray in house探测器Pilatus 100K
机构 / 束线SFB 1035, Technische Universität München / Rigaku BioSAXS-1000 · Garching, Germany
q 范围0.137 – 6.853样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:151 个字段值
字段路径原始值
codeSASDCE3
statusPublished
type_of_curveMerged
angular_unit1/A
project.titleSegmental, Domain-Selective Perdeuteration and Small-Angle Neutron Scattering for Structural Analysis of Multi-Domain Proteins.
project.publication.titleSegmental, Domain-Selective Perdeuteration and Small-Angle Neutron Scattering for Structural Analysis of Multi-Domain Proteins.
project.publication.author_listSonntag M, Jagtap PKA, Simon B, Appavou MS, Geerlof A, Stehle R, Gabel F, Hennig J, Sattler M
project.publication.journalAngew Chem Int Ed Engl
project.publication.doi10.1002/anie.201702904
project.publication.pmid28636238
project.publication.published_date2017 Aug 1
project.statusreleased
project.submitted_date2017-05-04
project.released_date2017-07-24
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experiment.instrument.detector.resolution172.0
experiment.instrument.nameSFB 1035, Technische Universität München
experiment.instrument.cityGarching
experiment.instrument.countryGermany
experiment.instrument.beamline_nameRigaku BioSAXS-1000
experiment.instrument.beam_geometrypoint
experiment.instrument.type_of_sourceX-ray in house
experiment.instrument.point_sourceTrue
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energy8.0405
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_namepoly U 15mer
experiment.sample.molecule[0].short_nameU15
experiment.sample.molecule[0].sequenceUUUUUUUUUUUUUUU
experiment.sample.molecule[0].organismnull
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeRNA
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw4.611
experiment.sample.molecule[0].total_mw4.611
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_nameNucleolysin TIA-1 isoform p40
experiment.sample.molecule[1].short_nameTIA-1 C-LPQTG
experiment.sample.molecule[1].sequenceMEDEMPKTLYVGNLSRDVTEALILQLFSQIGPCKNCKMIMDTAGNDPYCF VEFHEHRHAAAALAAMNGRKIMGKEVKVNWATTPSSQKLPQTGNHFHVFV GDLSPEITTEDIKAAFAPFGRISDARVVKDMATGKSKGYGFVSFFNKWDA ENAIQQMGGQWLGGRQIRTNWATRKPPAPKSTYESNTKQLSYDEVVNQSS PSNCTVYCGGVTSGLTEQLMRQTFSPFGQIMEIRVFPDKGYSFVRFNSHE SAAHAIVSVNGTTIEGHVVKCYWGK
experiment.sample.molecule[1].organismHomo sapiens
experiment.sample.molecule[1].uniprot_codeP31483
experiment.sample.molecule[1].uniprot_range_firstnull
experiment.sample.molecule[1].uniprot_range_lastnull
experiment.sample.molecule[1].oligomerizationmonomer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequenceMEDEMPKTLYVGNLSRDVTEALILQLFSQIGPCKNCKMIMDTAGNDPYCFVEFHEHRHAA AALAAMNGRKIMGKEVKVNWATTPSSQKKDTSSSTVVSTQRSQDHFHVFVGDLSPEITTE DIKAAFAPFGRISDARVVKDMATGKSKGYGFVSFFNKWDAENAIQQMGGQWLGGRQIRTN WATRKPPAPKSTYESNTKQLSYDEVVNQSSPSNCTVYCGGVTSGLTEQLMRQTFSPFGQI MEIRVFPDKGYSFVRFNSHESAAHAIVSVNGTTIEGHVVKCYWGKETLDMINPVQQQNQI GYPQPYGQWGQWYGNAQQIGQYMPNGWQVPAYGMYGQAWNQQGFNQTQSSAPWMGPNYGV QPPQGQNGSMLPNQPSGYRVAGYETQ
experiment.sample.molecule[1].mw30.522
experiment.sample.molecule[1].total_mw30.522
experiment.sample.molecule[1].number_molecules1
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
experiment.sample.buffer.name10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph6.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameNucleolysin TIA-1 isoform p40 in complex with U15 RNA (LPQTG containing construct for sortase mediated protein ligation)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressMunich, Germany
experiment.contributor[0].affiliation[0].full_nameTechnical University Munich
experiment.contributor[0].affiliation[0].webpagehttps://www.tum.de
experiment.contributor[0].contributor_nameMiriam
experiment.contributor[0].contributor_surnameSonntag
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2015-12-01
experiment.storage_temperaturenull
experiment.cell_temperature20.0
experiment.exposure_time900.0
experiment.number_of_frames8
experiment.wavelength0.1542
experiment.sample_detector_distancenull
experiment.concentration_min1.0
experiment.concentration_max5.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.137
experiment.s_max6.853
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionSAXS data from solutions of Nucleolysin TIA-1 isoform p40 in complex with U15 RNA (LPQTG containing construct for sortase mediated protein ligation) in 10 mM potassium phosphate, 50 mM NaCl, 10 mM DTT, pH 6 were collected using a Rigaku BioSAXS-1000 instrument (Munich, Germany) equipped with a Pilatus 100K detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). X-ray wavelength = unknown Sample-to-detector distance = unknown Sample concentration(s) = unknown
tags[]
intensity_unitnull
experimental_mw49.4
experimental_mw_errornull
guinier_i0_mw49.4
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i00.038
pddf_i0_error0.0002
guinier_i00.0378
guinier_i0_error0.0001
pddf_rg2.42
pddf_rg_error0.01
guinier_rg2.41
guinier_rg_error0.01
pddf_dmax8.6
pddf_dmax_errornull
porod_volume40.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first8
guinier_point_last28
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDCE3_datloglog_img.png
symmetrynull
last_modified2023-12-14T13:41:02.534142+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDCE3
statussuccess
started_at2026-08-11T13:58:22.825440+00:00
finished_at2026-08-11T13:58:30.270522+00:00
source_last_modified2023-12-14T13:41:02.534142+01:00
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查看完整 summary.json 原文
{
  "code": "SASDCE3",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/A",
  "project": {
    "title": "Segmental, Domain-Selective Perdeuteration and Small-Angle Neutron Scattering for Structural Analysis of Multi-Domain Proteins.",
    "publication": {
      "title": "Segmental, Domain-Selective Perdeuteration and Small-Angle Neutron Scattering for Structural Analysis of Multi-Domain Proteins.",
      "author_list": "Sonntag M, Jagtap PKA, Simon B, Appavou MS, Geerlof A, Stehle R, Gabel F, Hennig J, Sattler M",
      "journal": "Angew Chem Int Ed Engl",
      "doi": "10.1002/anie.201702904",
      "pmid": "28636238",
      "published_date": "2017 Aug 1"
    },
    "status": "released",
    "submitted_date": "2017-05-04",
    "released_date": "2017-07-24"
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          "molecule_source": "biological",
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      ],
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        "name": "10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT",
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        "comment": null,
        "additive": null,
        "concentration": null,
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        "ph": 6.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Nucleolysin TIA-1 isoform p40 in complex with U15 RNA (LPQTG containing construct for sortase mediated protein ligation)",
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      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Munich, Germany",
            "full_name": "Technical University Munich",
            "webpage": "https://www.tum.de"
          }
        ],
        "contributor_name": "Miriam",
        "contributor_surname": "Sonntag",
        "orcid": null
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    "concentration_method": null,
    "concentration_unit": null,
    "date": "2015-12-01",
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    "exposure_time": 900.0,
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    "flow_rate": null,
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    "total_exposure_time": null,
    "seccolumn": null
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  "fits": [],
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  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": null,
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "SAXS data from solutions of Nucleolysin TIA-1 isoform p40 in complex with U15 RNA (LPQTG containing construct for sortase mediated protein ligation) in 10 mM potassium phosphate, 50 mM NaCl, 10 mM DTT, pH 6 were collected using a Rigaku BioSAXS-1000 instrument (Munich, Germany) equipped with a Pilatus 100K detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).\r\n\r\nX-ray wavelength = unknown\r\nSample-to-detector distance = unknown\r\nSample concentration(s) = unknown",
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  "porod_volume": 40.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 8,
  "guinier_point_last": 28,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDCE3_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2023-12-14T13:41:02.534142+01:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
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