SASDCE5

Light encoded DNA biosensor: e13A DNA

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Merged 最后更新:2019-12-06T13:07:15.624532+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Light encoded DNA biosensor: e13A DNA

浓度0.25 – 2.0 缓冲液 / pH154 mM NaCl / 8.3
实验温度5.0 设备 / 束线ESRF / BM29
波长0.1 nm曝光0.05 s × 20

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
e13A
查看序列
CCACGCCAAACGCTGAAGGGCTTCTTCCTTATTTTTGGCGTGGCCACGCCAAACG
DNA———–—monomer分子数 116.903 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1976[3]0.290952–4.49359 1/nm含误差列缺失 06sasbdb/entries/e5/sasdce5/source/SASDCE5.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)8.00.5nm
i0Guinier32.03.0—
i0P(r)32.03.0—
mwExperimental15.0—kDa
mwGuinier I(0)14.02.0kDa
mwPorod17.0—kDa
porod_volumePorod24.0—nm³
rgGuinier2.320.03nm
rgP(r)2.360.04nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称154 mM NaCl缓冲液浓度—
pH8.3添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2013-11-22储存 / 测量温度5.0 / 5.0
曝光时间0.05帧数20
波长0.1样品-探测器距离3.0
光源X-ray synchrotron探测器Pilatus 1M
机构 / 束线ESRF / BM29 · Grenoble, France
q 范围0.291 – 4.494样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDCE5
statusPublished
type_of_curveMerged
angular_unit1/nm
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project.publication.titleOptical and Structural Characterization of a Chronic Myeloid Leukemia DNA Biosensor.
project.publication.author_listCordeiro M, Otrelo-Cardoso AR, Svergun DI, Konarev PV, Lima JC, Santos-Silva T, Baptista PV
project.publication.journalACS Chem Biol
project.publication.doi10.1021/acschembio.8b00029
project.publication.pmid29562136
project.publication.published_date2018 May 18
project.statusreleased
project.submitted_date2017-06-23
project.released_date2018-04-03
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experiment.instrument.countryFrance
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experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
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pddf_softwareATSAS GNOM
pddf_software_versionGNOM45
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of Light encoded DNA biosensor: e13A DNA in 154 mM NaCl, pH 8.3 were collected at the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 0.3 and 2 mg/ml were measured at 5°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve. The model depicts the averaged spatial representation of the DNA (DAMFILT occupancy and volume-corrected bead model).
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last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
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字段路径原始值
codeSASDCE5
statussuccess
started_at2026-08-11T14:46:14.699633+00:00
finished_at2026-08-11T14:46:22.913091+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
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  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/nm",
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    "title": "Optical and Structural Characterization of a Chronic Myeloid Leukemia DNA Biosensor.",
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      "author_list": "Cordeiro M, Otrelo-Cardoso AR, Svergun DI, Konarev PV, Lima JC, Santos-Silva T, Baptista PV",
      "journal": "ACS Chem Biol",
      "doi": "10.1021/acschembio.8b00029",
      "pmid": "29562136",
      "published_date": "2018 May 18"
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  "experiment_description": "Synchrotron SAXS data from solutions of Light encoded DNA biosensor: e13A DNA in 154 mM NaCl, pH 8.3 were collected at the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 0.3 and 2 mg/ml were measured at 5°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve. The model depicts the averaged spatial representation of the DNA (DAMFILT occupancy and volume-corrected bead model).",
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  "pddf_point_first": null,
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  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDCE5_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
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