Atg1-Atg13 Subcomplex

{
  "code": "SASDCL3",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Solution structure of the Atg1 complex: implications for the architecture of the phagophore assembly site.",
    "publication": {
      "title": "Solution structure of the Atg1 complex: implications for the architecture of the phagophore assembly site.",
      "author_list": "Köfinger J, Ragusa MJ, Lee IH, Hummer G, Hurley JH",
      "journal": "Structure",
      "doi": "10.1016/j.str.2015.02.012",
      "pmid": "25817386",
      "published_date": "2015 May 5"
    },
    "status": "released",
    "submitted_date": "2017-05-18",
    "released_date": "2017-05-05"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDCL3.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDCL3.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCL3_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCL3_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCL3_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCL3_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDCL3.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus3 X 2M",
        "resolution": 172.0
      },
      "name": "Advanced Light Source (ALS)",
      "city": "Berkeley, CA",
      "country": "USA",
      "beamline_name": "12.3.1 (SIBYLS)",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Serine/threonine-protein kinase ATG1",
          "short_name": "Atg1",
          "sequence": "DSNITPAVESLAAKAFVMYSFAEMKFSQILPTPPSSTDYDPLSDKRLSNGSCAIEDEEDLDQGRPPSNQ\r\nTLTSATTKISSATNVDTQIPAPELKKLCTESLLLYLKALTILAASMKLTSKWWYENESKNCTLKLNILVQ\r\nWIRDRFNECLDKAEFLRLKLHAINTSPNSQWSDDDPVIFVEKLIYDRALDISRNAARMEMESGNYNTCEL\r\nAYATSLWMLEILLDENFQFNEVYDDEYASNITSLDESDKEMIKKYISSIANRLKALKSKMV",
          "organism": "Kluyveromyces lactis",
          "uniprot_code": "Q6CSX2",
          "uniprot_range_first": 562,
          "uniprot_range_last": 831,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MSSESHDKVVAKAIRLPTENYSVEKEIGKGSFAVVYKGLSLRDGRNIAIKAVSRSKLKNK\nKLLENLEVEIAILKKIKHPHIVGLIDCERTSSDFYLIMEYCALGDLTFFIKKRKNLVLKH\nPLIKTVFEHYPPPSTEHNGLNRVLVVNYLQQLSSALKFLRSKNLVHRDIKPQNLLLCTPL\nLDYNDPKTFHELGFVGIYNLPILKIADFGFARFLPNTSLAETLCGSPLYMAPEILNYQKY\nNAKADLWSVGTVLYEMCCGRPPFKASNHLELFQKIKKANDEITVPSNCYIEPKLFNLIRG\nLLTFDPDSRMGFTDFFNNEVVTEDLTRYEQSYEPDLESKSKDVAESNMFVSEYLVKPLKQ\nQESAHIPPTQTDENTSVQTGVRRTSGKERLATNHPPHQQIHPEDNSQNPEQSYQSASQKR\nLKSSYNDLILEKEYVVVEKKTVEVNSLADDFANNGPITNNQGAQVIKPLRYRTSSSSDAS\nGGRRASLVERRLSISSLSPSNALSKALGLASVRLFGYQHNTKATSSPPQQTLLNPQIFQE\nLTENAVLRADHKLNPFSEQMLDSNITPAVESLAAKAFVMYSFAEMKFSQILPTPPSSTDY\nDPLSDKRLSNGSCAIEDEEDLDQGRPPSNQTLTSATTKISSATNVDTQIPAPELKKLCTE\nSLLLYLKALTILAASMKLTSKWWYENESKNCTLKLNILVQWIRDRFNECLDKAEFLRLKL\nHAINTSPNSQWSDDDPVIFVEKLIYDRALDISRNAARMEMESGNYNTCELAYATSLWMLE\nILLDENFQFNEVYDDEYASNITSLDESDKEMIKKYISSIANRLKALKSKMV",
          "mw": 30.664,
          "total_mw": 61.328,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        },
        {
          "long_name": "Autophagy-related protein 13",
          "short_name": "Atg13",
          "sequence": "FDRTAKPRKSTENPPEDLLEF\r\nVKLLEDKKELNMKPNTILPQQDISNSLMRFQSMKSNNDALSDNLSMSMSIDQPNV",
          "organism": "Kluyveromyces lactis",
          "uniprot_code": "Q6CWK2",
          "uniprot_range_first": 400,
          "uniprot_range_last": 475,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MSEGEVQVIELINNFFLKSALLLEQSKVAHNFDTEEALRDGNHLFNIETRGDPLLEAQIQ\nPWITFDGVKTMPPLVIETYLDLRALQPNHMVYLHDADGNPWMVCKGGKKTEIVLERWLVE\nLDKQTIDDSIDSNDPENLHKQLVLLFRYLYTLTQLLPANDIITKPHNSQQPALINVQTRL\nLDGSKPILSKGRVGLSKPIIASYSNTMNETNIASHLEQRKITPIKTTFGSLRITVSYRKD\nVDFYVIDSDDLQKRYMTPSLSNETTTVPDRRASSNCSRSMSVSPKTNTINATLFPLEGSS\nARRQSISSKLQPFKVGSVGSGSFVQSGSAQSTTSLVPSLSRNVSSSSVVAALKVQRGSAG\nSTIVNGDVPPELSSVGSGSKYSSSFGRIRRHSSIRRSESFDRTAKPRKSTENPPEDLLEF\nVKLLEDKKELNMKPNTILPQQDISNSLMRFQSMKSNNDALSDNLSMSMSIDQPNVRFGSN\nSHSPIPSFSPNYGSIPSRLSQGSRNNSNAELITSRKSSLDRHKHNLLSRTGSNVDINRRG\nSVGTMETTNEDSKEDEQSHMKGMHFSNEATINKDDDEDEMLMKRSFNAGTSTTEQVMGSP\nRSIRSISVSSYPRNQLPLKHLNLSHPTTSATTTHAKFHKSEMSPDPLHTEGAQPHTSSQH\nHNSSQKNDEDDDLLFVMSDMNLTN",
          "mw": 8.726,
          "total_mw": 17.452,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "20 mM Tris, 200 mM NaCl, 2% glycerol",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Atg1-Atg13 Subcomplex",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Hanover, NH, USA",
            "full_name": "Dartmouth College",
            "webpage": "http://home.dartmouth.edu/"
          }
        ],
        "contributor_name": "Michael",
        "contributor_surname": "Ragusa",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2013-12-10",
    "storage_temperature": null,
    "cell_temperature": 22.0,
    "exposure_time": null,
    "number_of_frames": null,
    "wavelength": null,
    "sample_detector_distance": null,
    "concentration_min": 1.0,
    "concentration_max": 5.2,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.089,
    "s_max": 2.719,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": null,
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of the Atg1-Atg13 Subcomplex in 20 mM Tris, 200 mM NaCl, 2% glycerol, pH 8 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector (I(s) vs s, where s = 4πsin θ/λ and 2θ is the scattering angle). Solute concentrations ranging between 1 and 5.2 mg/ml were measured at 22°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.",
  "tags": [],
  "intensity_unit": null,
  "experimental_mw": 78.5,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": null,
  "porod_mw_error": null,
  "pddf_i0": 1013.0,
  "pddf_i0_error": 2.2,
  "guinier_i0": 1019.45,
  "guinier_i0_error": 2.2,
  "pddf_rg": 3.3,
  "pddf_rg_error": null,
  "guinier_rg": 3.32,
  "guinier_rg_error": 0.2,
  "pddf_dmax": 10.9,
  "pddf_dmax_error": null,
  "porod_volume": 160.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 11,
  "guinier_point_last": 60,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDCL3_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2020-11-24T17:34:01.079622+01:00",
  "bragg_peak": []
}

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  "status": "success",
  "started_at": "2026-08-11T14:02:39.101319+00:00",
  "finished_at": "2026-08-11T14:02:46.808044+00:00",
  "source_last_modified": "2020-11-24T17:34:01.079622+01:00",
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