SASDCL5

Light encoded DNA biosensor: e14Ae13Be14C DNA

Data type: SASBDB experimental data Status: Published Curve type: Merged Last updated: 2019-12-06T13:07:15.624532+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Light encoded DNA biosensor: e14Ae13Be14C DNA

Concentration0.25 – 2.0 Buffer / pH154 mM NaCl / 8.3
Experimental temperature10.0 Facility / beamlineESRF / BM29
Wavelength0.1 nmExposure0.05 s × 20

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
e14Ae13Be14C
View sequence
CCACGCCAAACGCTGAAGGGCTTCTTCCTTATTTTTGGCGTGGCCACGCCAAACGCTGAAGGGCTTCTTCCTTATTTTTGGCGTGGCCACGCCAAACGCTGA
DNA———–—monomerMolecule count 131.377 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11020[3]0.143143–4.95184 1/nmHas uncertainty columnMissing 076sasbdb/entries/l5/sasdcl5/source/SASDCL5.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)14.01.0nm
i0Guinier73.2960.67—
i0P(r)75.05.0—
mwExperimental35.0—kDa
mwPorod33.0—kDa
porod_volumePorod42.0—nm³
rgGuinier3.430.05nm
rgP(r)3.790.05nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name154 mM NaClBuffer concentration—
pH8.3Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2016-06-16Storage / measurement temperature10.0 / 10.0
Exposure time0.05Frames20
Wavelength0.1Sample-to-detector distance3.0
SourceX-ray synchrotronDetectorPilatus 1M
Facility / beamlineESRF / BM29 · Grenoble, France
q range0.143 – 4.952Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:156 field values
Field pathRaw value
codeSASDCL5
statusPublished
type_of_curveMerged
angular_unit1/nm
project.titleOptical and Structural Characterization of a Chronic Myeloid Leukemia DNA Biosensor.
project.publication.titleOptical and Structural Characterization of a Chronic Myeloid Leukemia DNA Biosensor.
project.publication.author_listCordeiro M, Otrelo-Cardoso AR, Svergun DI, Konarev PV, Lima JC, Santos-Silva T, Baptista PV
project.publication.journalACS Chem Biol
project.publication.doi10.1021/acschembio.8b00029
project.publication.pmid29562136
project.publication.published_date2018 May 18
project.statusreleased
project.submitted_date2017-06-23
project.released_date2018-04-03
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experiment.instrument.detector.resolution172.0
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometry
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experiment.instrument.point_sourcenull
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experiment.sample.molecule[0].long_namee14Ae13Be14C
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceccacgccaaacgctgaagggcttcttccttatttttggcgtggccacgccaaacgctgaagggcttcttccttatttttggcgtggccacgccaaacgctga
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experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeDNA
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw31.377
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experiment.sample.buffer.concentration_unitnull
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experiment.sample.purity_methodnull
experiment.sample.nameLight encoded DNA biosensor: e14Ae13Be14C DNA
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
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experiment.sample.absorbptionnull
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experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_namePetr
experiment.contributor[0].contributor_surnameKonarev
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experiment.concentration_methodnull
experiment.concentration_unitnull
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experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time0.05
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experiment_descriptionSynchrotron SAXS data from solutions of Light encoded DNA biosensor: e14Ae13Be14C DNA in 154 mM NaCl, pH 8.3 were collected at the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 0.3 and 2 mg/ml were measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve. The model depicts the averaged spatial representation of the DNA (DAMFILT occupancy and volume-corrected bead model).
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last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
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Field pathRaw value
codeSASDCL5
statussuccess
started_at2026-08-11T14:50:13.297462+00:00
finished_at2026-08-11T14:50:21.725142+00:00
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View the complete summary.json source
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  "status": "Published",
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  "angular_unit": "1/nm",
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    "title": "Optical and Structural Characterization of a Chronic Myeloid Leukemia DNA Biosensor.",
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      "author_list": "Cordeiro M, Otrelo-Cardoso AR, Svergun DI, Konarev PV, Lima JC, Santos-Silva T, Baptista PV",
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  "experiment_description": "Synchrotron SAXS data from solutions of Light encoded DNA biosensor: e14Ae13Be14C DNA in 154 mM NaCl, pH 8.3 were collected at the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 0.3 and 2 mg/ml were measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve. The model depicts the averaged spatial representation of the DNA (DAMFILT occupancy and volume-corrected bead model).",
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  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDCL5_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
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}
View the complete manifest.json source
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