SASDCL6

Lys63-linked dimer ubiquitin

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2019-12-06T13:07:15.624532+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Lys63-linked dimer ubiquitin

浓度— – 5.1 缓冲液 / pH100mM NaCl, 10mM sodium acetate / 6.0
实验温度25.0 设备 / 束线Shanghai Synchrotron Radiation Facility (SSRF) / BL19U2
波长0.1033 nm曝光1.0 s × 20

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Polyubiquitin-C
查看序列
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
proteinHomo sapiensP0CG48305–379dimer分子数 28.565 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1933[3]0.0123022366–0.469875795 1/A含误差列缺失 00sasbdb/entries/l6/sasdcl6/source/SASDCL6.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)6.5—Å
i0Guinier69.06640.01—
i0P(r)69.00.07—
mwExperimental14.0—kDa
mwPorod14.0—kDa
porod_volumePorod23.8—ų
rgGuinier2.0840.01Å
rgP(r)2.1150.024Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称100mM NaCl, 10mM sodium acetate缓冲液浓度—
pH6.0添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2016-03-24储存 / 测量温度4.0 / 25.0
曝光时间1.0帧数20
波长0.1033样品-探测器距离2.126
光源X-ray synchrotron探测器Pilatus 1M
机构 / 束线Shanghai Synchrotron Radiation Facility (SSRF) / BL19U2 · Shanghai, China
q 范围0.123 – 4.699样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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full_entry_zipsasbdb/entries/l6/sasdcl6/source/SASDCL6.zipdownloaded282921a5aa2e5c664ba9b5a749271142dd8502667c6d07e0e09e0d3405f4f7aab0cc2下载查看原文件源站
pddfsasbdb/entries/l6/sasdcl6/source/SASDCL6.outdownloaded436053bd075c8f66aa2e5d8931e8379d9a79cf8f42d1f46823b833c2666b01071cfb9下载查看原文件源站
sascifsasbdb/entries/l6/sasdcl6/source/SASDCL6.sascifdownloaded83750c8d443a54d3fc6332eb913b1187e5eb87af2468d137b5809226f24b542515e90下载查看原文件源站
summarysasbdb/entries/l6/sasdcl6/source/summary.jsondownloaded66659946d3344b10062a2b3c61f52593643ff2bdb6da9df8ee93f6b087fcd9eeff05下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:134 个字段值
字段路径原始值
codeSASDCL6
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleLys63-linked ubiquitin chain adopts multiple conformational states for specific target recognition.
project.publication.titleLys63-linked ubiquitin chain adopts multiple conformational states for specific target recognition.
project.publication.author_listLiu Z, Gong Z, Jiang WX, Yang J, Zhu WK, Guo DC, Zhang WP, Liu ML, Tang C
project.publication.journalElife
project.publication.doi10.7554/eLife.05767
project.publication.pmid26090905
project.publication.published_date2015 Jun 19
project.statusreleased
project.submitted_date2017-07-18
project.released_date2017-07-18
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDCL6.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDCL6.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCL6_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCL6_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCL6_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCL6_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDCL6.sascif
experiment.instrument.detector.typeDectris
experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameShanghai Synchrotron Radiation Facility (SSRF)
experiment.instrument.cityShanghai
experiment.instrument.countryChina
experiment.instrument.beamline_nameBL19U2
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_namePolyubiquitin-C
experiment.sample.molecule[0].short_nameubiquitin
experiment.sample.molecule[0].sequenceMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP0CG48
experiment.sample.molecule[0].uniprot_range_first305
experiment.sample.molecule[0].uniprot_range_last379
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLI FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKA KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKT ITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR LRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL SDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIE NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTL TGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLH LVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLED GRTLSDYNIQKESTLHLVLRLRGGV
experiment.sample.molecule[0].mw8.565
experiment.sample.molecule[0].total_mw17.13
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name100mM NaCl, 10mM sodium acetate
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph6.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameLys63-linked dimer ubiquitin
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressWuhan, China
experiment.contributor[0].affiliation[0].full_nameWuhan Institute of Physics and Mathematics of the Chinese Academy of Sciences
experiment.contributor[0].affiliation[0].webpagehttp://english.wipm.cas.cn/
experiment.contributor[0].contributor_nameZhu
experiment.contributor[0].contributor_surnameLiuzhu
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2016-03-24
experiment.storage_temperature4.0
experiment.cell_temperature25.0
experiment.exposure_time1.0
experiment.number_of_frames20
experiment.wavelength0.1033
experiment.sample_detector_distance2.126
experiment.concentration_minnull
experiment.concentration_max5.1
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.123
experiment.s_max4.699
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of 0.3mM K63-diUb in 100mM NaCl, 10mM sodium acetate, pH 6 were collected on the BL19U2 camera on the storage ring Shanghai Synchrotron Radiation Facility (SSRF) (Shanghai, China). Using a Pilatus 1M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1033 nm one solute concentration of 5.1 mg/ml was measured at 25°C. 20 successive 1 second frames were collected, the data were normalized to the intensity of the transmitted beam and radially averaged, the scattering of the solvent-blank was subtracted. The angular range between 0.271 and 2.282 1/nm was used for PDDF conversion.
tags[]
intensity_unitnull
experimental_mw14.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw14.0
porod_mw_errornull
pddf_i069.0
pddf_i0_error0.07
guinier_i069.0664
guinier_i0_error0.01
pddf_rg2.115
pddf_rg_error0.024
guinier_rg2.084
guinier_rg_error0.01
pddf_dmax6.5
pddf_dmax_errornull
porod_volume23.8
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first31
guinier_point_last101
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDCL6_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDCL6
statussuccess
started_at2026-08-11T15:11:44.330977+00:00
finished_at2026-08-11T15:11:52.081376+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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查看完整 summary.json 原文
{
  "code": "SASDCL6",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Lys63-linked ubiquitin chain adopts multiple conformational states for specific target recognition.",
    "publication": {
      "title": "Lys63-linked ubiquitin chain adopts multiple conformational states for specific target recognition.",
      "author_list": "Liu Z, Gong Z, Jiang WX, Yang J, Zhu WK, Guo DC, Zhang WP, Liu ML, Tang C",
      "journal": "Elife",
      "doi": "10.7554/eLife.05767",
      "pmid": "26090905",
      "published_date": "2015 Jun 19"
    },
    "status": "released",
    "submitted_date": "2017-07-18",
    "released_date": "2017-07-18"
  },
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  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCL6_pofr_img.png",
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  "experiment": {
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        "resolution": 172.0
      },
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      "city": "Shanghai",
      "country": "China",
      "beamline_name": "BL19U2",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
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      "line_collimation_integrationwidth": null,
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    },
    "sample": {
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          "long_name": "Polyubiquitin-C",
          "short_name": "ubiquitin",
          "sequence": "MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG",
          "organism": "Homo sapiens",
          "uniprot_code": "P0CG48",
          "uniprot_range_first": 305,
          "uniprot_range_last": 379,
          "oligomerization": "dimer",
          "molecular_type": "protein",
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          "mw": 8.565,
          "total_mw": 17.13,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
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        "concentration_unit": null,
        "comment": null,
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        "pka": null,
        "ph": 6.0,
        "deuteration": null
      },
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      "name": "Lys63-linked dimer ubiquitin",
      "ext_coefficient": null,
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    },
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            "address": "Wuhan, China",
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        "orcid": null
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  "intensities_log_log_plot": "SASDCL6_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
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}
查看完整 manifest.json 原文
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