Synchrotron SAXS data from solutions of 0.3mM K63-diUb in 100mM NaCl, 10mM sodium acetate, pH 6 were collected on the BL19U2 camera on the storage ring Shanghai Synchrotron Radiation Facility (SSRF) (Shanghai, China). Using a Pilatus 1M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1033 nm one solute concentration of 5.1 mg/ml was measured at 25°C. 20 successive 1 second frames were collected, the data were normalized to the intensity of the transmitted beam and radially averaged, the scattering of the solvent-blank was subtracted. The angular range between 0.271 and 2.282 1/nm was used for PDDF conversion.
相关论文
标题
Lys63-linked ubiquitin chain adopts multiple conformational states for specific target recognition.
作者
Liu Z, Gong Z, Jiang WX, Yang J, Zhu WK, Guo DC, Zhang WP, Liu ML, Tang C
Wuhan Institute of Physics and Mathematics of the Chinese Academy of Sciences
experiment.contributor[0].affiliation[0].webpage
http://english.wipm.cas.cn/
experiment.contributor[0].contributor_name
Zhu
experiment.contributor[0].contributor_surname
Liuzhu
experiment.contributor[0].orcid
null
experiment.concentration_method
null
experiment.concentration_unit
null
experiment.date
2016-03-24
experiment.storage_temperature
4.0
experiment.cell_temperature
25.0
experiment.exposure_time
1.0
experiment.number_of_frames
20
experiment.wavelength
0.1033
experiment.sample_detector_distance
2.126
experiment.concentration_min
null
experiment.concentration_max
5.1
experiment.sample_volume
null
experiment.flow_rate
null
experiment.s_min
0.123
experiment.s_max
4.699
experiment.total_exposure_time
null
experiment.seccolumn
null
fits
[]
estimated_volume_method
null
pddf_software
ATSAS GNOM
pddf_software_version
null
i0_calibration_standard
null
description
null
experiment_description
Synchrotron SAXS data from solutions of 0.3mM K63-diUb in 100mM NaCl, 10mM sodium acetate, pH 6 were collected on the BL19U2 camera on the storage ring Shanghai Synchrotron Radiation Facility (SSRF) (Shanghai, China). Using a Pilatus 1M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1033 nm one solute concentration of 5.1 mg/ml was measured at 25°C. 20 successive 1 second frames were collected, the data were normalized to the intensity of the transmitted beam and radially averaged, the scattering of the solvent-blank was subtracted. The angular range between 0.271 and 2.282 1/nm was used for PDDF conversion.