The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.
Every source field is expanded here, including empty values, list members and fields already shown above.
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{
"code": "SASDCL7",
"status": "Published",
"type_of_curve": "Other",
"angular_unit": "1/A",
"project": {
"title": "Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors.",
"publication": {
"title": "Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors.",
"author_list": "Malaby AW, Das S, Chakravarthy S, Irving TC, Bilsel O, Lambright DG",
"journal": "Structure",
"doi": "10.1016/j.str.2017.11.019",
"pmid": "29276036",
"published_date": "2018 Jan 2"
},
"status": "released",
"submitted_date": "2017-09-22",
"released_date": "2018-01-02"
},
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}
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"contributor": [
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"affiliation": [
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"short_name": "UMASS",
"address": "Worcester, MA, USA",
"full_name": "UMASS Medical School",
"webpage": "https://www.umassmed.edu/"
}
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}
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"experiment_description": "This experimental profile was derived from SEC-SAXS data collected at the BioCAT Sector 18-ID beamline at the Argonne National Laboratory Advanced Photon Source using a MAR 165 CCD detector. The protein was equilibrated with a 1.2 molar excess of inositol 1,3,4,5-tetrakis phosphate (IP4), concentrated to 17 mg/ml, and injected in 0.15 ml onto a 24 ml Superdex-200 column equilibrated with 20 mM Tris, pH 8.0, 150 mM NaCl, 2 mM MgCl2, and 0.001 ml IP4. SAXS data sets were acquired with 1 s exposures at 5 s intervals during elution at a flow rate of 0.5 ml/min. Raw SAXS images were radially averaged on a log scale over the q range 0.00825-0.413 Å-1 and normalized by the incident beam intensity. The protein scattering profile was reconstructed by singular value decomposition and linear combination (SVD-LC) as described in Malaby et al. (2015) Methods for analysis of size-exclusion chromatography-small angle X-ray scattering and reconstruction of protein scattering. J Appl Crystallogr 48: 1102-1113.",
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"last_modified": "2020-08-28T15:28:09.113239+02:00",
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}
