Vaccinia virus DNA polymerase

{
  "code": "SASDCN5",
  "status": "Published",
  "type_of_curve": "Other",
  "angular_unit": "1/nm",
  "project": {
    "title": "The vaccinia virus DNA polymerase structure provides insights into the mode of processivity factor binding.",
    "publication": {
      "title": "The vaccinia virus DNA polymerase structure provides insights into the mode of processivity factor binding.",
      "author_list": "Tarbouriech N, Ducournau C, Hutin S, Mas PJ, Man P, Forest E, Hart DJ, Peyrefitte CN, Burmeister WP, Iseni F",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-017-01542-z",
      "pmid": "29129932",
      "published_date": "2017 Nov 13"
    },
    "status": "released",
    "submitted_date": "2017-06-27",
    "released_date": "2017-11-20"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDCN5.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDCN5.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCN5_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCN5_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCN5_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCN5_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDCN5.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": "Dectris",
        "name": "Pilatus 1M",
        "resolution": 172.0
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "DNA polymerase E9",
          "short_name": "E9",
          "sequence": "GAMDPDVRCINWFESHGENRFLYLKSRCRNGETVFIRFPHYFYYVVTDEIYQSLSPPPFNARPLGKMRTIDIDETISYNL\r\nDIKDRKCSVADMWLIEEPKKRSIQNATMDEFLNISWFYISNGISPDGCYSLDEQYLTKINNGCYHCDDPRNCFAKKIPRF\r\nDIPRSYLFLDIECHFDKKFPSVFINPISHTSYCYIDLSGKRLLFTLINEEMLTEQEIQEAVDRGCLRIQSLMEMDYEREL\r\nVLCSEIVLLRIAKQLLELTFDYVVTFNGHNFDLRYITNRLELLTGEKIIFRSPDKKEAVHLCIYERNQSSHKGVGGMANT\r\nTFHVNNNNGTIFFDLYSFIQKSEKLDSYKLDSISKNAFSCMGKVLNRGVREMTFIGDDTTDAKGKAAAFAKVLTTGNYVT\r\nVDEDIICKVIRKDIWENGFKVVLLCPTLPNDTYKLSFGKDDVDLAQMYKDYNLNIALDMARYCIHDACLCQYLWEYYGVE\r\nTKTDAGASTYVLPQSMVFEYRASTVIKGPLLKLLLETKTILVRSETKQKFPYEGGKVFAPKQKMFSNNVLIFDYNSLYPN\r\nVCIFGNLSPETLVGVVVSTNRLEEEINNQLLLQKYPPPRYITVHCEPRLPNLISEIAIFDRSIEGTIPRLLRTFLAERAR\r\nYKKMLKQATSSTEKAIYDSMQYTYKIVANSVYGLMGFRNSALYSYASAKSCTSIGRRMILYLESVLNGAELSNGMLRFAN\r\nPLSNPFYMDDRDINPIVKTSLPIDYRFRFRSVYGDTDSVFTEIDSQDVDKSIEIAKELERLINNRVLFNNFKIEFEAVYK\r\nNLIMQSKKKYTTMKYSASSNSKSVPERINKGTSETRRDVSKFHKNMIKTYKTRLSEMLSEGRMNSNQVCIDILRSLETDL\r\nRSEFDSRSSPLELFMLSRMHHSNYKSADNPNMYLVTEYNKNNPETIELGERYYFAYICPANVPWTKKLVNIKTYETIIDR\r\nSFKLGSDQRIFYEVYFKRLTSEIVNLLDNKVLCISFFERMFGSKPTFYEA",
          "organism": "Vaccinia virus",
          "uniprot_code": "P20509",
          "uniprot_range_first": 1,
          "uniprot_range_last": 1006,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MDVRCINWFESHGENRFLYLKSRCRNGETVFIRFPHYFYYVVTDEIYQSLSPPPFNARPL\nGKMRTIDIDETISYNLDIKDRKCSVADMWLIEEPKKRSIQNATMDEFLNISWFYISNGIS\nPDGCYSLDEQYLTKINNGCYHCDDPRNCFAKKIPRFDIPRSYLFLDIECHFDKKFPSVFI\nNPISHTSYCYIDLSGKRLLFTLINEEMLTEQEIQEAVDRGCLRIQSLMEMDYERELVLCS\nEIVLLRIAKQLLELTFDYVVTFNGHNFDLRYITNRLELLTGEKIIFRSPDKKEAVHLCIY\nERNQSSHKGVGGMANTTFHVNNNNGTIFFDLYSFIQKSEKLDSYKLDSISKNAFSCMGKV\nLNRGVREMTFIGDDTTDAKGKAAAFAKVLTTGNYVTVDEDIICKVIRKDIWENGFKVVLL\nCPTLPNDTYKLSFGKDDVDLAQMYKDYNLNIALDMARYCIHDACLCQYLWEYYGVETKTD\nAGASTYVLPQSMVFEYRASTVIKGPLLKLLLETKTILVRSETKQKFPYEGGKVFAPKQKM\nFSNNVLIFDYNSLYPNVCIFGNLSPETLVGVVVSTNRLEEEINNQLLLQKYPPPRYITVH\nCEPRLPNLISEIAIFDRSIEGTIPRLLRTFLAERARYKKMLKQATSSTEKAIYDSMQYTY\nKIVANSVYGLMGFRNSALYSYASAKSCTSIGRRMILYLESVLNGAELSNGMLRFANPLSN\nPFYMDDRDINPIVKTSLPIDYRFRFRSVYGDTDSVFTEIDSQDVDKSIEIAKELERLINN\nRVLFNNFKIEFEAVYKNLIMQSKKKYTTMKYSASSNSKSVPERINKGTSETRRDVSKFHK\nNMIKTYKTRLSEMLSEGRMNSNQVCIDILRSLETDLRSEFDSRSSPLELFMLSRMHHSNY\nKSADNPNMYLVTEYNKNNPETIELGERYYFAYICPANVPWTKKLVNIKTYETIIDRSFKL\nGSDQRIFYEVYFKRLTSEIVNLLDNKVLCISFFERMFGSKPTFYEA",
          "mw": 117.242,
          "total_mw": 117.242,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "20 mM Tris HCl, 100 mM NaCl, 4 mM DTT",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Vaccinia virus DNA polymerase",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "IBS",
            "address": "Grenoble, France",
            "full_name": "Institut de Biologie Structurale",
            "webpage": ""
          }
        ],
        "contributor_name": "Wim",
        "contributor_surname": "Burmeister",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2014-11-27",
    "storage_temperature": 4.0,
    "cell_temperature": 20.0,
    "exposure_time": 1.0,
    "number_of_frames": 900,
    "wavelength": 0.0992,
    "sample_detector_distance": 2.864,
    "concentration_min": null,
    "concentration_max": null,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.079,
    "s_max": 2.638,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [
    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDCN5_fit1_model1_img.png",
          "software": "PyMOL",
          "pdb_link": [
            {
              "pdb_code": "5n2e",
              "ext_code": "5N2E",
              "ext_type": "pdb",
              "difference_with_model": "identical"
            }
          ],
          "model_title": null,
          "type_of_model": "atomic",
          "software_version": "1.6",
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDCN5_fit1_model1.pdb",
          "model_mw": 116.1,
          "bead_radius": 1.7,
          "log": "https://www.sasbdb.org/media/log_files/e9.log",
          "symmetry": "none",
          "comment": "Calculated scattering curve of protein",
          "user": 226
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDCN5_fit1_fit_img.png",
      "software": "CRYSOL",
      "chi_square_value": 0.569,
      "p_value": 0.0,
      "fit_residual_plot": null,
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDCN5_fit1.fit",
      "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/e9.log",
      "software_version": "2.8.3",
      "description": null
    }
  ],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of Vaccinia virus DNA polymerase in 20 mM Tris HCl, 100 mM NaCl, 4 mM DTT, pH 7 using size exclusion chromatography coupled SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.0992 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Data were collected at at 20°C during the course of elution (900 successive 1 second frames). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the resulting subtracted curves were scaled and averaged.",
  "tags": [],
  "intensity_unit": null,
  "experimental_mw": 117.0,
  "experimental_mw_error": null,
  "guinier_i0_mw": 0.0,
  "guinier_i0_mw_error": null,
  "porod_mw": 117.0,
  "porod_mw_error": null,
  "pddf_i0": 27.0,
  "pddf_i0_error": 0.09,
  "guinier_i0": 19.97,
  "guinier_i0_error": 0.05,
  "pddf_rg": 3.55,
  "pddf_rg_error": 0.01,
  "guinier_rg": 3.9,
  "guinier_rg_error": 0.2,
  "pddf_dmax": 12.0,
  "pddf_dmax_error": null,
  "porod_volume": 202.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 1,
  "guinier_point_last": 45,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDCN5_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
  "bragg_peak": []
}

{
  "code": "SASDCN5",
  "status": "success",
  "started_at": "2026-08-11T14:51:36.414353+00:00",
  "finished_at": "2026-08-11T14:51:45.422066+00:00",
  "source_last_modified": "2019-12-06T13:07:15.624532+01:00",
  "files": [
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/rest-api/entry/summary/SASDCN5/",
      "path": "/data/saxsdb/sasbdb/entries/n5/sasdcn5/source/summary.json",
      "size": 9292,
      "sha256": "3322c372f1f7d6d7e780914e244a2962bb0041ff43a9ab3084b2c2cd26729001"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/intensities_files/SASDCN5.dat",
      "path": "/data/saxsdb/sasbdb/entries/n5/sasdcn5/source/SASDCN5.dat",
      "size": 24404,
      "sha256": "4bad9de58338b790f0198bdf9579ed6c4c0f40b2bcacd3ef389793eccf360d8a",
      "role": "curve"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/p_of_R_files/SASDCN5.out",
      "path": "/data/saxsdb/sasbdb/entries/n5/sasdcn5/source/SASDCN5.out",
      "size": 45755,
      "sha256": "c1431772dbea1def0093d3d603f60fbbd9336cee5dc8f1aaedd18a5f651f3462",
      "role": "pddf"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDCN5.sascif",
      "path": "/data/saxsdb/sasbdb/entries/n5/sasdcn5/source/SASDCN5.sascif",
      "size": 943055,
      "sha256": "104d847aa1789c2899a89dc18700e91d3506f350667f7b58836abc7d6c3ba01d",
      "role": "sascif"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/zip_directories/SASDCN5.zip",
      "path": "/data/saxsdb/sasbdb/entries/n5/sasdcn5/source/SASDCN5.zip",
      "size": 193102,
      "sha256": "a5baf5144d77a48b4161f507c804e3d9b133f6a8d204b0c41340cc8611b33b6b",
      "role": "full_entry_zip",
      "zip_member_count": 4,
      "zip_validation": "central_directory_readable"
    }
  ]
}